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Induction of phytoestrogen production in Medicago sativa leaves by irrigation with sewage water
Year:
1995
Authors :
Badani, Hanna
;
.
Kapulnik, Yoram
;
.
Wininger, Smadar
;
.
Volume :
35
Co-Authors:
Shore, L.S., Department of Hormone Research, Kimron Veterinary Institute, Bet Dagan POB 12, Israel
Kapulnik, Y., Department of Legume Inoculation, Volcani Center, POB 6, Bet Dagan, Israel
Gurevich, M., Department of Hormone Research, Kimron Veterinary Institute, Bet Dagan POB 12, Israel
Wininger, S., Department of Legume Inoculation, Volcani Center, POB 6, Bet Dagan, Israel
Badamy, H., Department of Legume Inoculation, Volcani Center, POB 6, Bet Dagan, Israel
Shemesh, M., Department of Hormone Research, Kimron Veterinary Institute, Bet Dagan POB 12, Israel
Facilitators :
From page:
363
To page:
369
(
Total pages:
7
)
Abstract:
The phytoestrogenic activity of alfalfa irrigated with sewage water was determined by radioreceptor assay (RRA) and high pressure liquid chromatography (HPLC). It was found that irrigation of alfalfa fields increased the phytoestrogenic activity measured in the leaves from 10 ng estrogen equivalents (est. eqv.) to 137 ng est. eqv. g-1, which was principally due to a rise in coumestrol (<10 μg to 60 μg g-1). Similarly, when plants grown in greenhouses under controlled conditions were irrigated with sewage water, the phytoestrogenic content rose from 34 to 48 est. eqv. g-1; (P<0.01; n = 16). Under hydroponic conditions, addition of 1 nM of estrone to the media resulted in a significant rise in phytoestrogen content in the leaves within 48 hr (from 21.2 to 34.5 est. eqv. g-1; P<0.05, n = 10). Alfalfa irrigated with sewage water may therefore have levels of coumestrol (<30 μg g-1) which can affect fertility in cattle. The agents in the sewage water responsible for increased phytoestrogen content of the alfalfa are probably the steroidal estrogens, estradiol and estrone, which are present in sewage in concentrations ranging from 10 to 300 ng l-1. © 1995.
Note:
Related Files :
Medicago sativa
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More details
DOI :
10.1016/0098-8472(95)00010-4
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
18907
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:25
Scientific Publication
Induction of phytoestrogen production in Medicago sativa leaves by irrigation with sewage water
35
Shore, L.S., Department of Hormone Research, Kimron Veterinary Institute, Bet Dagan POB 12, Israel
Kapulnik, Y., Department of Legume Inoculation, Volcani Center, POB 6, Bet Dagan, Israel
Gurevich, M., Department of Hormone Research, Kimron Veterinary Institute, Bet Dagan POB 12, Israel
Wininger, S., Department of Legume Inoculation, Volcani Center, POB 6, Bet Dagan, Israel
Badamy, H., Department of Legume Inoculation, Volcani Center, POB 6, Bet Dagan, Israel
Shemesh, M., Department of Hormone Research, Kimron Veterinary Institute, Bet Dagan POB 12, Israel
Induction of phytoestrogen production in Medicago sativa leaves by irrigation with sewage water
The phytoestrogenic activity of alfalfa irrigated with sewage water was determined by radioreceptor assay (RRA) and high pressure liquid chromatography (HPLC). It was found that irrigation of alfalfa fields increased the phytoestrogenic activity measured in the leaves from 10 ng estrogen equivalents (est. eqv.) to 137 ng est. eqv. g-1, which was principally due to a rise in coumestrol (<10 μg to 60 μg g-1). Similarly, when plants grown in greenhouses under controlled conditions were irrigated with sewage water, the phytoestrogenic content rose from 34 to 48 est. eqv. g-1; (P<0.01; n = 16). Under hydroponic conditions, addition of 1 nM of estrone to the media resulted in a significant rise in phytoestrogen content in the leaves within 48 hr (from 21.2 to 34.5 est. eqv. g-1; P<0.05, n = 10). Alfalfa irrigated with sewage water may therefore have levels of coumestrol (<30 μg g-1) which can affect fertility in cattle. The agents in the sewage water responsible for increased phytoestrogen content of the alfalfa are probably the steroidal estrogens, estradiol and estrone, which are present in sewage in concentrations ranging from 10 to 300 ng l-1. © 1995.
Scientific Publication
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