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Fructokinase and hexokinase from pollen grains of bell pepper (Capsicum annuum L.): Possible role in pollen germination under conditions of high temperature and CO2 enrichment
Year:
2002
Source of publication :
Annals of Botany
Authors :
Aloni, Benyamin
;
.
Karni, Leah
;
.
Volume :
90
Co-Authors:
Karni, L., Department of Vegetable Crops, Institute of Field and Garden Crops, Volcani Center, Bet Dagan 50250, Israel
Aloni, B., Department of Vegetable Crops, Institute of Field and Garden Crops, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
607
To page:
612
(
Total pages:
6
)
Abstract:
The processes of pollen grain development and germination depend on the uptake and metabolism of pollen sugars. In pepper (Capsicum annuum L.), initial sugar metabolism includes sucrose hydrolysis by invertase and subsequent phosphorylation of glucose and fructose by hexose kinases. The main objective of this study was to investigate changes in fructokinase (EC 2.7.1.4) and hexokinase (EC.2.7.1.1) activities in pepper flowers during their development, and to study the possible roles of these enzymes in determining pollen germination capacity under high temperature and under CO2 enrichment, previously shown to modify sugar concentrations in pepper pollen (Aloni et al., 2001 Physiologia Plantarum 112: 505-512). Fructokinase (FK) activity was predominant in pepper pollen, and increased during pollen maturation. Pollen hexokinase (HK) activity was low and did not change throughout pollen development. High-temperature treatment (day/night, 32/26°C) of pepper plants reduced the percentage of pollen that germinated compared with that under normal temperatures (26/22°C), and concomitantly reduced the activity of FK in mature pollen. High temperature also reduced FK and HK activity in the anther. Under high ambient CO2 (800 μl 1-1) pollen FK activity was enhanced, The results suggest that pollen and anther FK may play a role in the regulation of pollen germination, possibly by providing fructose-6-phosphate for glycolysis, or through conversion to UDP-glucose (UDPG) to support the biosynthesis of cell wall material for pollen tube growth. High temperature stress and CO2 enrichment may influence pollen germination capacity by affecting these pathways. © 2002 Annals of Botany Company.
Note:
Related Files :
Capsicum annuum
carbohydrate metabolism
Fructokinase
germination
Growth, Development and Aging
metabolism
pollen (external)
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More details
DOI :
10.1093/aob/mcf234
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
18943
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:25
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Scientific Publication
Fructokinase and hexokinase from pollen grains of bell pepper (Capsicum annuum L.): Possible role in pollen germination under conditions of high temperature and CO2 enrichment
90
Karni, L., Department of Vegetable Crops, Institute of Field and Garden Crops, Volcani Center, Bet Dagan 50250, Israel
Aloni, B., Department of Vegetable Crops, Institute of Field and Garden Crops, Volcani Center, Bet Dagan 50250, Israel
Fructokinase and hexokinase from pollen grains of bell pepper (Capsicum annuum L.): Possible role in pollen germination under conditions of high temperature and CO2 enrichment
The processes of pollen grain development and germination depend on the uptake and metabolism of pollen sugars. In pepper (Capsicum annuum L.), initial sugar metabolism includes sucrose hydrolysis by invertase and subsequent phosphorylation of glucose and fructose by hexose kinases. The main objective of this study was to investigate changes in fructokinase (EC 2.7.1.4) and hexokinase (EC.2.7.1.1) activities in pepper flowers during their development, and to study the possible roles of these enzymes in determining pollen germination capacity under high temperature and under CO2 enrichment, previously shown to modify sugar concentrations in pepper pollen (Aloni et al., 2001 Physiologia Plantarum 112: 505-512). Fructokinase (FK) activity was predominant in pepper pollen, and increased during pollen maturation. Pollen hexokinase (HK) activity was low and did not change throughout pollen development. High-temperature treatment (day/night, 32/26°C) of pepper plants reduced the percentage of pollen that germinated compared with that under normal temperatures (26/22°C), and concomitantly reduced the activity of FK in mature pollen. High temperature also reduced FK and HK activity in the anther. Under high ambient CO2 (800 μl 1-1) pollen FK activity was enhanced, The results suggest that pollen and anther FK may play a role in the regulation of pollen germination, possibly by providing fructose-6-phosphate for glycolysis, or through conversion to UDP-glucose (UDPG) to support the biosynthesis of cell wall material for pollen tube growth. High temperature stress and CO2 enrichment may influence pollen germination capacity by affecting these pathways. © 2002 Annals of Botany Company.
Scientific Publication
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