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Carbon regulation of environmental pH by secreted small molecules that modulate pathogenicity in phytopathogenic fungi
Year:
2016
Source of publication :
Molecular Plant Pathology
Authors :
Dubey, Amit K.
;
.
Glam, Nofar
;
.
Ment, Dana
;
.
Prusky, Dov
;
.
Volume :
17
Co-Authors:
Bi, F., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, China
Barad, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel, Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Ment, D., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Luria, N., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Dubey, A., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Casado, V., Department of Microbiology and Genetics, CIALE, Universidad de Salamanca, Salamanca, Spain
Glam, N., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel, Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Mínguez, J.D., Department of Microbiology and Genetics, CIALE, Universidad de Salamanca, Salamanca, Spain
Espeso, E.A., Department of Molecular and Cellular Biology, Centro de Investigaciones Biológicas (C.I.B.), Madrid, Spain
Fluhr, R., Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel
Prusky, D., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Bi, F., Department of Postharvest Science of Fresh ProduceAgricultural Research Organization, The Volcani CenterBet Dagan 50250 Israel, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, and Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of AgricultureGuangzhou510640 China
Barad, S., Department of Postharvest Science of Fresh ProduceAgricultural Research Organization, The Volcani CenterBet Dagan 50250 Israel, Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and EnvironmentThe Hebrew University of JerusalemRehovot76100 Israel
Ment, D., Department of Postharvest Science of Fresh ProduceAgricultural Research Organization, The Volcani CenterBet Dagan 50250 Israel
Luria, N., Department of Postharvest Science of Fresh ProduceAgricultural Research Organization, The Volcani CenterBet Dagan 50250 Israel
Dubey, A., Department of Postharvest Science of Fresh ProduceAgricultural Research Organization, The Volcani CenterBet Dagan 50250 Israel
Casado, V., Department of Microbiology and GeneticsCIALE, Universidad de SalamancaSalamanca37007 Spain
Glam, N., Department of Postharvest Science of Fresh ProduceAgricultural Research Organization, The Volcani CenterBet Dagan 50250 Israel, Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and EnvironmentThe Hebrew University of JerusalemRehovot76100 Israel
Mínguez, J.D., Department of Microbiology and GeneticsCIALE, Universidad de SalamancaSalamanca37007 Spain
Espeso, E.A., Department of Molecular and Cellular BiologyCentro de Investigaciones Biológicas (C.I.B.)Madrid28040 Spain
Fluhr, R., Department of Plant and Environmental SciencesWeizmann Institute of ScienceRehovot76100 Israel
Prusky, D., Department of Postharvest Science of Fresh ProduceAgricultural Research Organization, The Volcani CenterBet Dagan 50250 Israel
Facilitators :
From page:
1178
To page:
1195
(
Total pages:
18
)
Abstract:
Fruit pathogens can contribute to the acidification or alkalinization of the host environment. This capability has been used to divide fungal pathogens into acidifying and/or alkalinizing classes. Here, we show that diverse classes of fungal pathogens—Colletotrichum gloeosporioides, Penicillium expansum, Aspergillus nidulans and Fusarium oxysporum—secrete small pH-affecting molecules. These molecules modify the environmental pH, which dictates acidic or alkaline colonizing strategies, and induce the expression of PACC-dependent genes. We show that, in many organisms, acidification is induced under carbon excess, i.e. 175 mm sucrose (the most abundant sugar in fruits). In contrast, alkalinization occurs under conditions of carbon deprivation, i.e. less than 15 mm sucrose. The carbon source is metabolized by glucose oxidase (gox2) to gluconic acid, contributing to medium acidification, whereas catalysed deamination of non-preferred carbon sources, such as the amino acid glutamate, by glutamate dehydrogenase 2 (gdh2), results in the secretion of ammonia. Functional analyses of Δgdh2 mutants showed reduced alkalinization and pathogenicity during growth under carbon deprivation, but not in high-carbon medium or on fruit rich in sugar, whereas analysis of Δgox2 mutants showed reduced acidification and pathogencity under conditions of excess carbon. The induction pattern of gdh2 was negatively correlated with the expression of the zinc finger global carbon catabolite repressor creA. The present results indicate that differential pH modulation by fruit fungal pathogens is a host-dependent mechanism, affected by host sugar content, that modulates environmental pH to enhance fruit colonization. © 2015 BSPP and John Wiley & Sons Ltd
Note:
Related Files :
carbon regulation of pathogenicity
pathogenicity
pH regulation
Show More
Related Content
More details
DOI :
10.1111/mpp.12355
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19007
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:25
You may also be interested in
Scientific Publication
Carbon regulation of environmental pH by secreted small molecules that modulate pathogenicity in phytopathogenic fungi
17
Bi, F., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, China
Barad, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel, Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Ment, D., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Luria, N., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Dubey, A., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Casado, V., Department of Microbiology and Genetics, CIALE, Universidad de Salamanca, Salamanca, Spain
Glam, N., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel, Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Mínguez, J.D., Department of Microbiology and Genetics, CIALE, Universidad de Salamanca, Salamanca, Spain
Espeso, E.A., Department of Molecular and Cellular Biology, Centro de Investigaciones Biológicas (C.I.B.), Madrid, Spain
Fluhr, R., Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel
Prusky, D., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Bi, F., Department of Postharvest Science of Fresh ProduceAgricultural Research Organization, The Volcani CenterBet Dagan 50250 Israel, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, and Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of AgricultureGuangzhou510640 China
Barad, S., Department of Postharvest Science of Fresh ProduceAgricultural Research Organization, The Volcani CenterBet Dagan 50250 Israel, Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and EnvironmentThe Hebrew University of JerusalemRehovot76100 Israel
Ment, D., Department of Postharvest Science of Fresh ProduceAgricultural Research Organization, The Volcani CenterBet Dagan 50250 Israel
Luria, N., Department of Postharvest Science of Fresh ProduceAgricultural Research Organization, The Volcani CenterBet Dagan 50250 Israel
Dubey, A., Department of Postharvest Science of Fresh ProduceAgricultural Research Organization, The Volcani CenterBet Dagan 50250 Israel
Casado, V., Department of Microbiology and GeneticsCIALE, Universidad de SalamancaSalamanca37007 Spain
Glam, N., Department of Postharvest Science of Fresh ProduceAgricultural Research Organization, The Volcani CenterBet Dagan 50250 Israel, Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and EnvironmentThe Hebrew University of JerusalemRehovot76100 Israel
Mínguez, J.D., Department of Microbiology and GeneticsCIALE, Universidad de SalamancaSalamanca37007 Spain
Espeso, E.A., Department of Molecular and Cellular BiologyCentro de Investigaciones Biológicas (C.I.B.)Madrid28040 Spain
Fluhr, R., Department of Plant and Environmental SciencesWeizmann Institute of ScienceRehovot76100 Israel
Prusky, D., Department of Postharvest Science of Fresh ProduceAgricultural Research Organization, The Volcani CenterBet Dagan 50250 Israel
Carbon regulation of environmental pH by secreted small molecules that modulate pathogenicity in phytopathogenic fungi
Fruit pathogens can contribute to the acidification or alkalinization of the host environment. This capability has been used to divide fungal pathogens into acidifying and/or alkalinizing classes. Here, we show that diverse classes of fungal pathogens—Colletotrichum gloeosporioides, Penicillium expansum, Aspergillus nidulans and Fusarium oxysporum—secrete small pH-affecting molecules. These molecules modify the environmental pH, which dictates acidic or alkaline colonizing strategies, and induce the expression of PACC-dependent genes. We show that, in many organisms, acidification is induced under carbon excess, i.e. 175 mm sucrose (the most abundant sugar in fruits). In contrast, alkalinization occurs under conditions of carbon deprivation, i.e. less than 15 mm sucrose. The carbon source is metabolized by glucose oxidase (gox2) to gluconic acid, contributing to medium acidification, whereas catalysed deamination of non-preferred carbon sources, such as the amino acid glutamate, by glutamate dehydrogenase 2 (gdh2), results in the secretion of ammonia. Functional analyses of Δgdh2 mutants showed reduced alkalinization and pathogenicity during growth under carbon deprivation, but not in high-carbon medium or on fruit rich in sugar, whereas analysis of Δgox2 mutants showed reduced acidification and pathogencity under conditions of excess carbon. The induction pattern of gdh2 was negatively correlated with the expression of the zinc finger global carbon catabolite repressor creA. The present results indicate that differential pH modulation by fruit fungal pathogens is a host-dependent mechanism, affected by host sugar content, that modulates environmental pH to enhance fruit colonization. © 2015 BSPP and John Wiley & Sons Ltd
Scientific Publication
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