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Rickettsia influences thermotolerance in the whitefly Bemisia tabaci B biotype
Year:
2011
Source of publication :
Insect Science
Authors :
Brumin, Marina
;
.
Ghanim, Murad
;
.
Kontsedalov, Svetlana
;
.
Volume :
18
Co-Authors:
Brumin, M., Department of Entomology, Institute of Plant Protection, The Volcani Center, Bet Dagan, Israel
Kontsedalov, S., Department of Entomology, Institute of Plant Protection, The Volcani Center, Bet Dagan, Israel
Ghanim, M., Department of Entomology, Institute of Plant Protection, The Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
57
To page:
66
(
Total pages:
10
)
Abstract:
The whitefly Bemisia tabaci harbors Portiera aleyrodidarum, an obligatory symbiotic bacterium, as well as several secondary symbionts, including Rickettsia, Hamiltonella, Wolbachia, Arsenophonus, Cardinium and Fritschea, the function of which is unknown. In Israel, Rickettsia is found in both the B and Q of B. tabaci biotypes, and while all other secondary symbionts are located in the bacteriomes, Rickettsia can occupy most of the body cavity of the insect. We tested whether Rickettsia influences the biology of B. tabaci and found that exposing a Rickettsia-containing population to increasing temperatures significantly increases its tolerance to heat shock that reached 40°C, compared to a Rickettsia-free population. This increase in tolerance to heat shock was not associated with specific induction of heat-shock protein gene expression; however, it was associated with reduction in Rickettsia numbers as was assessed by quantitative real-time polymerase chain reaction and fluorescence in situ hybridization analyses. To assess the causes for thermotolerance when Rickettsia is reduced, we tested whether its presence is associated with the induction of genes required for thermotolerance. We found that under normal 25°C rearing temperature, genes associated with response to stress such as cytoskeleton genes are induced in the Rickettsia-containing population. Thus, the presence of Rickettsia in B. tabaci under normal conditions induces the expression of genes required for thermotolerance that under high temperatures indirectly lead to this tolerance. © 2011 The Authors Journal compilation © Institute of Zoology, Chinese Academy of Sciences.
Note:
Related Files :
Aleyrodidae
Bemisia tabaci
Candidatus Fritschea
gene expression
Wolbachia
Show More
Related Content
More details
DOI :
10.1111/j.1744-7917.2010.01396.x
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19019
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:25
You may also be interested in
Scientific Publication
Rickettsia influences thermotolerance in the whitefly Bemisia tabaci B biotype
18
Brumin, M., Department of Entomology, Institute of Plant Protection, The Volcani Center, Bet Dagan, Israel
Kontsedalov, S., Department of Entomology, Institute of Plant Protection, The Volcani Center, Bet Dagan, Israel
Ghanim, M., Department of Entomology, Institute of Plant Protection, The Volcani Center, Bet Dagan, Israel
Rickettsia influences thermotolerance in the whitefly Bemisia tabaci B biotype
The whitefly Bemisia tabaci harbors Portiera aleyrodidarum, an obligatory symbiotic bacterium, as well as several secondary symbionts, including Rickettsia, Hamiltonella, Wolbachia, Arsenophonus, Cardinium and Fritschea, the function of which is unknown. In Israel, Rickettsia is found in both the B and Q of B. tabaci biotypes, and while all other secondary symbionts are located in the bacteriomes, Rickettsia can occupy most of the body cavity of the insect. We tested whether Rickettsia influences the biology of B. tabaci and found that exposing a Rickettsia-containing population to increasing temperatures significantly increases its tolerance to heat shock that reached 40°C, compared to a Rickettsia-free population. This increase in tolerance to heat shock was not associated with specific induction of heat-shock protein gene expression; however, it was associated with reduction in Rickettsia numbers as was assessed by quantitative real-time polymerase chain reaction and fluorescence in situ hybridization analyses. To assess the causes for thermotolerance when Rickettsia is reduced, we tested whether its presence is associated with the induction of genes required for thermotolerance. We found that under normal 25°C rearing temperature, genes associated with response to stress such as cytoskeleton genes are induced in the Rickettsia-containing population. Thus, the presence of Rickettsia in B. tabaci under normal conditions induces the expression of genes required for thermotolerance that under high temperatures indirectly lead to this tolerance. © 2011 The Authors Journal compilation © Institute of Zoology, Chinese Academy of Sciences.
Scientific Publication
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