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Biolistic transformation of Cercospora caricis, a specific pathogenic fungus of Cyperus rotundus
Year:
2001
Source of publication :
Mycological Research
Authors :
Aly, Radi
;
.
Dor, Evgenia
;
.
Golan, Shmuel
;
.
Halpern, Nurit
;
.
Hershenhorn, Joseph
;
.
Volume :
105
Co-Authors:
Aly, R., Department of Weed Research, Agricultural Research Organization, Newe Ya’ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Halpern, N., Department of Weed Research, Agricultural Research Organization, Newe Ya’ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Rubin, B., Department of Field Crops, Vegetable and Genetics, Faculty of Agriculture, Rehovot 76100, Israel
Dor, E., Department of Weed Research, Agricultural Research Organization, Newe Ya’ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Golan, S., Department of Weed Research, Agricultural Research Organization, Newe Ya’ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Hershenhorn, J., Department of Weed Research, Agricultural Research Organization, Newe Ya’ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Facilitators :
From page:
150
To page:
152
(
Total pages:
3
)
Abstract:
Cercospora caricis is being considered as a bioherbicide agent for use against purple nutsedge (Cyperus rotundus), one of the world's worst weeds. However, first its efficiency must be improved, for example by genetic transformation. By optimizing physical and biological parameters, the particle gun acceleration method (biolistics) has been adapted to transform mycelial cells of C. caricis. Two genes were expressed in C. caricis by biolistic transformation. The β-glucuronidase gene (GUS) fused to the GDP1 promoter of Cochliobolus hetrostrophus and the hygromycin B resistance gene under control of the PtrpC promoter of Aspergillus nidulans. Although the transformation frequency was not high, all transformants were stable when they were propagated on a selective medium after eight subsequent transfers.
Note:
Related Files :
Aspergillus
fungi
genetic transformation
gene transfer
Hygromycin B
microprojectile bombardment
Show More
Related Content
More details
DOI :
10.1017/S095375620000349X
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19055
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:26
Scientific Publication
Biolistic transformation of Cercospora caricis, a specific pathogenic fungus of Cyperus rotundus
105
Aly, R., Department of Weed Research, Agricultural Research Organization, Newe Ya’ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Halpern, N., Department of Weed Research, Agricultural Research Organization, Newe Ya’ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Rubin, B., Department of Field Crops, Vegetable and Genetics, Faculty of Agriculture, Rehovot 76100, Israel
Dor, E., Department of Weed Research, Agricultural Research Organization, Newe Ya’ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Golan, S., Department of Weed Research, Agricultural Research Organization, Newe Ya’ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Hershenhorn, J., Department of Weed Research, Agricultural Research Organization, Newe Ya’ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Biolistic transformation of Cercospora caricis, a specific pathogenic fungus of Cyperus rotundus
Cercospora caricis is being considered as a bioherbicide agent for use against purple nutsedge (Cyperus rotundus), one of the world's worst weeds. However, first its efficiency must be improved, for example by genetic transformation. By optimizing physical and biological parameters, the particle gun acceleration method (biolistics) has been adapted to transform mycelial cells of C. caricis. Two genes were expressed in C. caricis by biolistic transformation. The β-glucuronidase gene (GUS) fused to the GDP1 promoter of Cochliobolus hetrostrophus and the hygromycin B resistance gene under control of the PtrpC promoter of Aspergillus nidulans. Although the transformation frequency was not high, all transformants were stable when they were propagated on a selective medium after eight subsequent transfers.
Scientific Publication
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