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Resistance to northern leaf blight in maize (Zea mays) conditioned by the HtN gene and the association with isoperoxidases
Year:
1998
Authors :
Bar-Tsur, Avri
;
.
Reuveni, Reuven
;
.
Shimoni, Moshe
;
.
Tadmor, Yaakov
;
.
Volume :
20
Co-Authors:

Bar-Zur, A., Dept. of Fld. Crops and Nat. Rsrc., Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Tadmor, Y., Dept. of Fld. Crops and Nat. Rsrc., Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Juvik, J.A., Department of Horticulture, University of Illinois, Urbana, IL 61801, United States
Shimoni, M., Department of Plant Pathology, ARO, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Reuveni, R., Department of Plant Pathology, ARO, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel

Facilitators :
From page:
28
To page:
34
(
Total pages:
7
)
Abstract:
The Htn gene confers resistance to northern leaf blight (NLB) caused by Exserohilum turcicum. Three maize (Zea mays) near isogenic lines (NILs), with and without the gene HtN, two sweet corn inbred lines (IL677a and H6314Htn), together with the parental, F1, F2, and backcross 1 (BC1) populations from a cross between the inbreds W64A and W22HtN, were artificially inoculated and evaluated in the greenhouse for comparative resistance to race 1 of E. turcicum. The number and the size of lesions developed on the genotypes carrying the HtN allele were significantly reduced in comparison with plants lacking the HtN allele. Resistance of plants carrying HtN was characterized by the formation of yellow or red spots at and around the sites of inoculation. Greenhouse studies were confirmed by field experiments. The association between isoperoxidases and HtN gene was studied in leaf tissue samples which were collected from seedlings of the parents, F1, BC1, and F2 HtN segregating populations, prior to inoculation. Polyacrylamide gel electrophoresis revealed distinct isoperoxidases for the susceptible and for the resistant inbred lines. A strong correlation was observed between individual plant-specific isozymes and plant expression of resistance conferred by the HtN allele in the segregating BC1 and F2 populations. These results suggest that, in the resistant parent, the HtN gene is linked to a locus coding for a specific isoperoxidase, or that the gene product of the HtN allele is this isoperoxidase itself. The potential use of peroxidase isozyme analysis as a marker for HtN is discussed.
Note:
Related Files :
backcrossing
disease resistance
host resistance
Inoculation
isoperoxidase
polyacrylamide gel electrophoresis
Zea mays
Show More
Related Content
More details
DOI :
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19100
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:26
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Scientific Publication
Resistance to northern leaf blight in maize (Zea mays) conditioned by the HtN gene and the association with isoperoxidases
20

Bar-Zur, A., Dept. of Fld. Crops and Nat. Rsrc., Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Tadmor, Y., Dept. of Fld. Crops and Nat. Rsrc., Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Juvik, J.A., Department of Horticulture, University of Illinois, Urbana, IL 61801, United States
Shimoni, M., Department of Plant Pathology, ARO, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Reuveni, R., Department of Plant Pathology, ARO, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel

Resistance to northern leaf blight in maize (Zea mays) conditioned by the HtN gene and the association with isoperoxidases
The Htn gene confers resistance to northern leaf blight (NLB) caused by Exserohilum turcicum. Three maize (Zea mays) near isogenic lines (NILs), with and without the gene HtN, two sweet corn inbred lines (IL677a and H6314Htn), together with the parental, F1, F2, and backcross 1 (BC1) populations from a cross between the inbreds W64A and W22HtN, were artificially inoculated and evaluated in the greenhouse for comparative resistance to race 1 of E. turcicum. The number and the size of lesions developed on the genotypes carrying the HtN allele were significantly reduced in comparison with plants lacking the HtN allele. Resistance of plants carrying HtN was characterized by the formation of yellow or red spots at and around the sites of inoculation. Greenhouse studies were confirmed by field experiments. The association between isoperoxidases and HtN gene was studied in leaf tissue samples which were collected from seedlings of the parents, F1, BC1, and F2 HtN segregating populations, prior to inoculation. Polyacrylamide gel electrophoresis revealed distinct isoperoxidases for the susceptible and for the resistant inbred lines. A strong correlation was observed between individual plant-specific isozymes and plant expression of resistance conferred by the HtN allele in the segregating BC1 and F2 populations. These results suggest that, in the resistant parent, the HtN gene is linked to a locus coding for a specific isoperoxidase, or that the gene product of the HtN allele is this isoperoxidase itself. The potential use of peroxidase isozyme analysis as a marker for HtN is discussed.
Scientific Publication
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