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The immediate-early protein IE0 of the Autographa californica nucleopolyhedrovirus is not essential for viral replication
Year:
2005
Source of publication :
Journal of Virology
Authors :
Chejanovsky, Nor
;
.
Rivkin, Hadassah
;
.
Volume :
79
Co-Authors:
Liqun, L., Entomology Department, Institute of Plant Protection, Volcani Center, POB 6, Bet Dagan 50250, Israel, Animal Health Biotechnology Unit, Temasek Life Sciences Laboratory, National University of Singapore, 1 Reserach Link, Singapore 117604, Singapore
Rivkin, H., Entomology Department, Institute of Plant Protection, Volcani Center, POB 6, Bet Dagan 50250, Israel
Chejanovsky, N., Entomology Department, Institute of Plant Protection, Volcani Center, POB 6, Bet Dagan 50250, Israel
Facilitators :
From page:
10077
To page:
10082
(
Total pages:
6
)
Abstract:
The role of the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) immediate-early protein IE0 in the baculoviral infection is not clear. In this study, we constructed the recombinant virus vAcΔie0 null for ie0 expression by targeted mutagenesis replacing exon0 with the cat gene. We found that vAcΔie0 replicated efficiently in Spodoptera littoralis SL2 cells, which are poorly permissive for AcMNPV. In contrast, in Spodoptera frugiperda SF9 cells, which are fully permissive for AcMNPV, vAcΔie0 DNA replication and budded virus production were delayed. These results and recently published data (X. Dai et al., J. Virol. 78:9633-9644, 2004) indicate that ie0 is not essential for AcMNPV replication but enhances it in permissive SF9 cells. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Note:
Related Files :
animal cell
Animals
Autographa californica
gene expression
unclassified drug
virus infection
virus replication
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Related Content
More details
DOI :
10.1128/JVI.79.15.10077-10082.2005
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19118
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:26
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Scientific Publication
The immediate-early protein IE0 of the Autographa californica nucleopolyhedrovirus is not essential for viral replication
79
Liqun, L., Entomology Department, Institute of Plant Protection, Volcani Center, POB 6, Bet Dagan 50250, Israel, Animal Health Biotechnology Unit, Temasek Life Sciences Laboratory, National University of Singapore, 1 Reserach Link, Singapore 117604, Singapore
Rivkin, H., Entomology Department, Institute of Plant Protection, Volcani Center, POB 6, Bet Dagan 50250, Israel
Chejanovsky, N., Entomology Department, Institute of Plant Protection, Volcani Center, POB 6, Bet Dagan 50250, Israel
The immediate-early protein IE0 of the Autographa californica nucleopolyhedrovirus is not essential for viral replication
The role of the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) immediate-early protein IE0 in the baculoviral infection is not clear. In this study, we constructed the recombinant virus vAcΔie0 null for ie0 expression by targeted mutagenesis replacing exon0 with the cat gene. We found that vAcΔie0 replicated efficiently in Spodoptera littoralis SL2 cells, which are poorly permissive for AcMNPV. In contrast, in Spodoptera frugiperda SF9 cells, which are fully permissive for AcMNPV, vAcΔie0 DNA replication and budded virus production were delayed. These results and recently published data (X. Dai et al., J. Virol. 78:9633-9644, 2004) indicate that ie0 is not essential for AcMNPV replication but enhances it in permissive SF9 cells. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Scientific Publication
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