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Temporally extended gene expression of the ADP-Glc pyrophosphorylase large subunit (AgpL1) leads to increased enzyme activity in developing tomato fruit
Year:
2006
Source of publication :
Planta
Authors :
Levin, Ilan
;
.
Petreikov, Marina
;
.
Schaffer, Arthur
;
.
Shen, Shmuel
;
.
Yeselson, Yelena
;
.
Volume :
224
Co-Authors:
Petreikov, M., Department of Vegetable Crops and Genetics, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Shen, S., Department of Vegetable Crops and Genetics, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Yeselson, Y., Department of Vegetable Crops and Genetics, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Levin, I., Department of Vegetable Crops and Genetics, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Bar, M., Gedera Seed Co., Gedera, Israel
Schaffer, A.A., Department of Vegetable Crops and Genetics, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
1465
To page:
1479
(
Total pages:
15
)
Abstract:
Tomato plants (Solanum lycopersicum) harboring the allele for the AGPase large subunit (AgpL1) derived from the wild species Solanum habrochaites (AgpL1 H ) are characterized by higher AGPase activity and increased starch content in the immature fruit, as well as higher soluble solids in the mature fruit following the breakdown of the transient starch, as compared to fruits from plants harboring the cultivated tomato allele (AgpL1 E ). Comparisons of AGPase subunit gene expression and protein levels during fruit development indicate that the increase in AGPase activity correlates with a prolonged expression of the AgpL1 gene in the AgpL1 H high starch line, leading to an extended presence of the L1 protein. The S1 (small subunit) protein also remained for an extended period of fruit development in the AgpL1 H fruit, linked to the presence of the L1 protein. There were no discernible differences between the kinetic characteristics of the partially purified AGPase-L1E and AGPase-L1H enzymes. The results indicate that the increased activity of AGPase in the AgpL1 H tomatoes is due to the extended expression of the regulatory L1 and to the subsequent stability of the heterotetramer in the presence of the L1 protein, implying a role for the large subunit not only in the allosteric control of AGPase activity but also in the stability of the AGPase L1-S1 heterotetramer. The introgression line of S. lycopersicum containing the wild species AgpL1 H allele is a novel example of transgressive heterosis in which the hybrid multimeric enzyme shows higher activity due to a modulated temporal expression of one of the subunits. © 2006 Springer-Verlag.
Note:
Related Files :
Base Sequence
Electrophoresis, Polyacrylamide Gel
Gene
Genetics
Growth, Development and Aging
metabolism
Solanum
Show More
Related Content
More details
DOI :
10.1007/s00425-006-0316-y
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19125
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:26
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Scientific Publication
Temporally extended gene expression of the ADP-Glc pyrophosphorylase large subunit (AgpL1) leads to increased enzyme activity in developing tomato fruit
224
Petreikov, M., Department of Vegetable Crops and Genetics, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Shen, S., Department of Vegetable Crops and Genetics, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Yeselson, Y., Department of Vegetable Crops and Genetics, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Levin, I., Department of Vegetable Crops and Genetics, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Bar, M., Gedera Seed Co., Gedera, Israel
Schaffer, A.A., Department of Vegetable Crops and Genetics, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Temporally extended gene expression of the ADP-Glc pyrophosphorylase large subunit (AgpL1) leads to increased enzyme activity in developing tomato fruit
Tomato plants (Solanum lycopersicum) harboring the allele for the AGPase large subunit (AgpL1) derived from the wild species Solanum habrochaites (AgpL1 H ) are characterized by higher AGPase activity and increased starch content in the immature fruit, as well as higher soluble solids in the mature fruit following the breakdown of the transient starch, as compared to fruits from plants harboring the cultivated tomato allele (AgpL1 E ). Comparisons of AGPase subunit gene expression and protein levels during fruit development indicate that the increase in AGPase activity correlates with a prolonged expression of the AgpL1 gene in the AgpL1 H high starch line, leading to an extended presence of the L1 protein. The S1 (small subunit) protein also remained for an extended period of fruit development in the AgpL1 H fruit, linked to the presence of the L1 protein. There were no discernible differences between the kinetic characteristics of the partially purified AGPase-L1E and AGPase-L1H enzymes. The results indicate that the increased activity of AGPase in the AgpL1 H tomatoes is due to the extended expression of the regulatory L1 and to the subsequent stability of the heterotetramer in the presence of the L1 protein, implying a role for the large subunit not only in the allosteric control of AGPase activity but also in the stability of the AGPase L1-S1 heterotetramer. The introgression line of S. lycopersicum containing the wild species AgpL1 H allele is a novel example of transgressive heterosis in which the hybrid multimeric enzyme shows higher activity due to a modulated temporal expression of one of the subunits. © 2006 Springer-Verlag.
Scientific Publication
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