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Pectolytic activity by the haustorium of the parasitic plant Orobanche L. (Orobanchaceae) in host roots
Year:
1998
Source of publication :
Annals of Botany
Authors :
Joel, Daniel M.
;
.
Losner-Goshen, Dalia
;
.
Portnoy, Vitaly H.
;
.
Volume :
81
Co-Authors:
Losner-Goshen, D., Department of Weed Research, Agricultural Research Organization, Newe-Ya'ar Research Center, P.O. Box 1021, Ramat-Yishay 30095, Israel
Portnoy, V.H., Department of Weed Research, Agricultural Research Organization, Newe-Ya'ar Research Center, P.O. Box 1021, Ramat-Yishay 30095, Israel
Mayer, A.M., Department of Botany, Hebrew University of Jerusalem, Jerusalem, Israel
Joel, D.M., Department of Weed Research, Agricultural Research Organization, Newe-Ya'ar Research Center, P.O. Box 1021, Ramat-Yishay 30095, Israel
Facilitators :
From page:
319
To page:
326
(
Total pages:
8
)
Abstract:
The possible involvement of enzymes in the penetration of intrusive cells of the parasitic angiosperm Orobanche into host root tissues was studied using cytochemical and immunocytochemical methods. Pectin methyl esterase (PME) was detected, with specific antibodies, in the cytoplasm and cell walls of Orobanche intrusive cells and in adjacent host apoplast. Depletion and chemical changes of pectins in host cell walls were shown by histochemical staining with PATAg, which detects carbohydrates that are sensitive to periodic acid, especially pectins, and with the monoclonal antibodies JIM 5 and JIM 7 that label pectins with low and high rates of esterification, respectively. Galacturonic sequences with low rates of esterification were more abundant in host cell walls adjacent to the parasite, which is consistent with pectin de-methylation by PME release from the parasite. Pectins were absent in middle lamellae and in host cell walls neighbouring mature intrusive cells of the parasite, consistent with further degradation of pectins by other enzymes. These results provide the first direct evidence for the presence and activity of a pectolytic enzyme in the infection zone of the haustorium of a parasitic angiosperm in situ.
Note:
Related Files :
broomrape
cell wall
Haustorium
Orobanche
Parasitic weed
pectin
Pectin methyl esterase
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More details
DOI :
10.1006/anbo.1997.0563
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19166
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:26
Scientific Publication
Pectolytic activity by the haustorium of the parasitic plant Orobanche L. (Orobanchaceae) in host roots
81
Losner-Goshen, D., Department of Weed Research, Agricultural Research Organization, Newe-Ya'ar Research Center, P.O. Box 1021, Ramat-Yishay 30095, Israel
Portnoy, V.H., Department of Weed Research, Agricultural Research Organization, Newe-Ya'ar Research Center, P.O. Box 1021, Ramat-Yishay 30095, Israel
Mayer, A.M., Department of Botany, Hebrew University of Jerusalem, Jerusalem, Israel
Joel, D.M., Department of Weed Research, Agricultural Research Organization, Newe-Ya'ar Research Center, P.O. Box 1021, Ramat-Yishay 30095, Israel
Pectolytic activity by the haustorium of the parasitic plant Orobanche L. (Orobanchaceae) in host roots
The possible involvement of enzymes in the penetration of intrusive cells of the parasitic angiosperm Orobanche into host root tissues was studied using cytochemical and immunocytochemical methods. Pectin methyl esterase (PME) was detected, with specific antibodies, in the cytoplasm and cell walls of Orobanche intrusive cells and in adjacent host apoplast. Depletion and chemical changes of pectins in host cell walls were shown by histochemical staining with PATAg, which detects carbohydrates that are sensitive to periodic acid, especially pectins, and with the monoclonal antibodies JIM 5 and JIM 7 that label pectins with low and high rates of esterification, respectively. Galacturonic sequences with low rates of esterification were more abundant in host cell walls adjacent to the parasite, which is consistent with pectin de-methylation by PME release from the parasite. Pectins were absent in middle lamellae and in host cell walls neighbouring mature intrusive cells of the parasite, consistent with further degradation of pectins by other enzymes. These results provide the first direct evidence for the presence and activity of a pectolytic enzyme in the infection zone of the haustorium of a parasitic angiosperm in situ.
Scientific Publication
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