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Sol-gel entrapment of monoclonal anti-atrazine antibodies
Year:
1996
Authors :
Aharonson, Nadav
;
.
Altstein, Miriam
;
.
Bronshtein, Alisa
;
.
Volume :
7
Co-Authors:
Turniansky, A., Department of Organic Chemistry, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Avnir, D., Department of Organic Chemistry, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Bronshtein, A., Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Aharonson, N., Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Altstein, M., Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
135
To page:
143
(
Total pages:
9
)
Abstract:
We report the successful doping of a sol-gel matrix with an antibody, retaining its ability to bind free antigen from an aqueous solution. The particular system described is monoclonal anti-atrazine mouse antibody which was entrapped in SiO2 sol-gel matrices, prepared from tetramethoxysilane by several methods. Atrazine was selected as a model compound for this study, within the framework of the development of immunochemical-based methods for monitoring pesticide residues and other organo-synthetic environmental contaminants. Nanogram quantities of atrazine were applied on SiO2 sol-gel columns doped with this antibody, and the amount of eluted antigen was determined by Enzyme Linked ImmunoSorbent Assay (ELISA). Under appropriate sol-gel-forming conditions, high amounts of atrazine were bound to the sol-gels, ranging between 60% and 91% of the amount applied to the column. The combination of the properties of the sol-gel matrix (e.g., stability, inertness, high porosity, high surface area and optical clarity), together with the selectivity and sensitivity of the antibodies, enable extension of this feasibility study to development of a novel group of immunosensors which could be used for purification, concentration and monitoring of a variety of residues from different sources. © 1996 Kluwer Academic Publishers.
Note:
Related Files :
Bioassay
chromatography
herbicides
immunoassay
Immunosensors
Silica
Silica sol-gel columns
Sol-gel
Sol-gel entrapment
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More details
DOI :
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19347
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:28
Scientific Publication
Sol-gel entrapment of monoclonal anti-atrazine antibodies
7
Turniansky, A., Department of Organic Chemistry, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Avnir, D., Department of Organic Chemistry, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Bronshtein, A., Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Aharonson, N., Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Altstein, M., Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Sol-gel entrapment of monoclonal anti-atrazine antibodies
We report the successful doping of a sol-gel matrix with an antibody, retaining its ability to bind free antigen from an aqueous solution. The particular system described is monoclonal anti-atrazine mouse antibody which was entrapped in SiO2 sol-gel matrices, prepared from tetramethoxysilane by several methods. Atrazine was selected as a model compound for this study, within the framework of the development of immunochemical-based methods for monitoring pesticide residues and other organo-synthetic environmental contaminants. Nanogram quantities of atrazine were applied on SiO2 sol-gel columns doped with this antibody, and the amount of eluted antigen was determined by Enzyme Linked ImmunoSorbent Assay (ELISA). Under appropriate sol-gel-forming conditions, high amounts of atrazine were bound to the sol-gels, ranging between 60% and 91% of the amount applied to the column. The combination of the properties of the sol-gel matrix (e.g., stability, inertness, high porosity, high surface area and optical clarity), together with the selectivity and sensitivity of the antibodies, enable extension of this feasibility study to development of a novel group of immunosensors which could be used for purification, concentration and monitoring of a variety of residues from different sources. © 1996 Kluwer Academic Publishers.
Scientific Publication
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