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Characterization and taxonomic implications of the rRNA genes of Mycobacterium leprae
Year:
1989
Source of publication :
Journal of Bacteriology
Authors :
Sela, Shlomo
;
.
Volume :
171
Co-Authors:
Sela, S., Department of Clinical Microbiology, Hebrew University Medical School, Jerusalem 91010, Israel
Clark-Curtiss, J.E., Department of Clinical Microbiology, Hebrew University Medical School, Jerusalem 91010, Israel
Bercovier, H., Department of Clinical Microbiology, Hebrew University Medical School, Jerusalem 91010, Israel
Facilitators :
From page:
70
To page:
73
(
Total pages:
4
)
Abstract:
The number of rRNA genes of Mycobacterium leprae was determined by restriction analysis of M. leprae total chromosomal DNA. A single set of rRNA genes was found. This set was subcloned from a cosmid library of M. leprae DNA into pUC13 and was characterized by restriction analysis and hybridization with Escherichia coli rRNA genes. The 16S, 23S, and 5S genes of M. leprae were clustered on a 5.3-kilobase DNA fragment. On one hand, restriction analysis of the set of rRNA genes showed the uniqueness of M. leprae among mycobacteria, but on the other hand, it suggested that M. leprae strains of several origins are very much alike. Quantitative hybridization studies between M. leprae rDNA and total DNA of various bacteria demonstrated a close relatedness between M. leprae and corynebacteria, nocardia, and mycobacteria, especially Mycobacterium tuberculosis.
Note:
Related Files :
DNA, Ribosomal
gene mapping
ribosome RNA
RNA, Ribosomal
Sequence Homology, Nucleic Acid
Species Specificity
Show More
Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19348
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:28
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Scientific Publication
Characterization and taxonomic implications of the rRNA genes of Mycobacterium leprae
171
Sela, S., Department of Clinical Microbiology, Hebrew University Medical School, Jerusalem 91010, Israel
Clark-Curtiss, J.E., Department of Clinical Microbiology, Hebrew University Medical School, Jerusalem 91010, Israel
Bercovier, H., Department of Clinical Microbiology, Hebrew University Medical School, Jerusalem 91010, Israel
Characterization and taxonomic implications of the rRNA genes of Mycobacterium leprae
The number of rRNA genes of Mycobacterium leprae was determined by restriction analysis of M. leprae total chromosomal DNA. A single set of rRNA genes was found. This set was subcloned from a cosmid library of M. leprae DNA into pUC13 and was characterized by restriction analysis and hybridization with Escherichia coli rRNA genes. The 16S, 23S, and 5S genes of M. leprae were clustered on a 5.3-kilobase DNA fragment. On one hand, restriction analysis of the set of rRNA genes showed the uniqueness of M. leprae among mycobacteria, but on the other hand, it suggested that M. leprae strains of several origins are very much alike. Quantitative hybridization studies between M. leprae rDNA and total DNA of various bacteria demonstrated a close relatedness between M. leprae and corynebacteria, nocardia, and mycobacteria, especially Mycobacterium tuberculosis.
Scientific Publication
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