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Distinct and conserved transcriptomic changes during nematode-induced giant cell development in tomato compared with Arabidopsis: A functional role for gene repression
Year:
2013
Source of publication :
New Phytologist
Authors :
Koltai, Hinanit
;
.
Resnick, Nathalie
;
.
Volume :
197
Co-Authors:
Portillo, M., Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avenida de Carlos III s/n, 45071 Toledo, Spain
Cabrera, J., Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avenida de Carlos III s/n, 45071 Toledo, Spain
Lindsey, K., Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, Durham University, Durham, DH1 3LE, United Kingdom
Topping, J., Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, Durham University, Durham, DH1 3LE, United Kingdom
Andrés, M.F., ICA CSIC, Protección Vegetal, Serrano 115 dpdo, Madrid, 28006, Spain
Emiliozzi, M., ICA CSIC, Protección Vegetal, Serrano 115 dpdo, Madrid, 28006, Spain
Oliveros, J.C., Centro Nacional de Biotecnología CSIC, Darwin 3, Campus Universidad Autónoma de, Madrid, 28049, Spain
García-Casado, G., Centro Nacional de Biotecnología CSIC, Darwin 3, Campus Universidad Autónoma de, Madrid, 28049, Spain
Solano, R., Centro Nacional de Biotecnología CSIC, Darwin 3, Campus Universidad Autónoma de, Madrid, 28049, Spain
Koltai, H., Institute of Plant Sciences ARO, Volcani Center, Bet-Dagan, 50250, Israel
Resnick, N., Institute of Plant Sciences ARO, Volcani Center, Bet-Dagan, 50250, Israel
Fenoll, C., Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avenida de Carlos III s/n, 45071 Toledo, Spain
Escobar, C., Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avenida de Carlos III s/n, 45071 Toledo, Spain
Facilitators :
From page:
1276
To page:
1290
(
Total pages:
15
)
Abstract:
Root-knot nematodes (RKNs) induce giant cells (GCs) from root vascular cells inside the galls. Accompanying molecular changes as a function of infection time and across different species, and their functional impact, are still poorly understood. Thus, the transcriptomes of tomato galls and laser capture microdissected (LCM) GCs over the course of parasitism were compared with those of Arabidopsis, and functional analysis of a repressed gene was performed. Microarray hybridization with RNA from galls and LCM GCs, infection-reproduction tests and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) transcriptional profiles in susceptible and resistant (Mi-1) lines were performed in tomato. Tomato GC-induced genes include some possibly contributing to the epigenetic control of GC identity. GC-repressed genes are conserved between tomato and Arabidopsis, notably those involved in lignin deposition. However, genes related to the regulation of gene expression diverge, suggesting that diverse transcriptional regulators mediate common responses leading to GC formation in different plant species. TPX1, a cell wall peroxidase specifically involved in lignification, was strongly repressed in GCs/galls, but induced in a nearly isogenic Mi-1 resistant line on nematode infection. TPX1 overexpression in susceptible plants hindered nematode reproduction and GC expansion. Time-course and cross-species comparisons of gall and GC transcriptomes provide novel insights pointing to the relevance of gene repression during RKN establishment. © 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.
Note:
Related Files :
Animal
Animals
arabidopsis
gene expression
Genetics
hybridization
metabolism
Parasitology
Plant Cell
Show More
Related Content
More details
DOI :
10.1111/nph.12121
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19374
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:28
Scientific Publication
Distinct and conserved transcriptomic changes during nematode-induced giant cell development in tomato compared with Arabidopsis: A functional role for gene repression
197
Portillo, M., Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avenida de Carlos III s/n, 45071 Toledo, Spain
Cabrera, J., Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avenida de Carlos III s/n, 45071 Toledo, Spain
Lindsey, K., Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, Durham University, Durham, DH1 3LE, United Kingdom
Topping, J., Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, Durham University, Durham, DH1 3LE, United Kingdom
Andrés, M.F., ICA CSIC, Protección Vegetal, Serrano 115 dpdo, Madrid, 28006, Spain
Emiliozzi, M., ICA CSIC, Protección Vegetal, Serrano 115 dpdo, Madrid, 28006, Spain
Oliveros, J.C., Centro Nacional de Biotecnología CSIC, Darwin 3, Campus Universidad Autónoma de, Madrid, 28049, Spain
García-Casado, G., Centro Nacional de Biotecnología CSIC, Darwin 3, Campus Universidad Autónoma de, Madrid, 28049, Spain
Solano, R., Centro Nacional de Biotecnología CSIC, Darwin 3, Campus Universidad Autónoma de, Madrid, 28049, Spain
Koltai, H., Institute of Plant Sciences ARO, Volcani Center, Bet-Dagan, 50250, Israel
Resnick, N., Institute of Plant Sciences ARO, Volcani Center, Bet-Dagan, 50250, Israel
Fenoll, C., Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avenida de Carlos III s/n, 45071 Toledo, Spain
Escobar, C., Facultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla-La Mancha, Avenida de Carlos III s/n, 45071 Toledo, Spain
Distinct and conserved transcriptomic changes during nematode-induced giant cell development in tomato compared with Arabidopsis: A functional role for gene repression
Root-knot nematodes (RKNs) induce giant cells (GCs) from root vascular cells inside the galls. Accompanying molecular changes as a function of infection time and across different species, and their functional impact, are still poorly understood. Thus, the transcriptomes of tomato galls and laser capture microdissected (LCM) GCs over the course of parasitism were compared with those of Arabidopsis, and functional analysis of a repressed gene was performed. Microarray hybridization with RNA from galls and LCM GCs, infection-reproduction tests and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) transcriptional profiles in susceptible and resistant (Mi-1) lines were performed in tomato. Tomato GC-induced genes include some possibly contributing to the epigenetic control of GC identity. GC-repressed genes are conserved between tomato and Arabidopsis, notably those involved in lignin deposition. However, genes related to the regulation of gene expression diverge, suggesting that diverse transcriptional regulators mediate common responses leading to GC formation in different plant species. TPX1, a cell wall peroxidase specifically involved in lignification, was strongly repressed in GCs/galls, but induced in a nearly isogenic Mi-1 resistant line on nematode infection. TPX1 overexpression in susceptible plants hindered nematode reproduction and GC expansion. Time-course and cross-species comparisons of gall and GC transcriptomes provide novel insights pointing to the relevance of gene repression during RKN establishment. © 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.
Scientific Publication
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