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Yolk sphere formation is initiated in oocytes before development of patency in follicles of the moth, Plodia interpunctella
Year:
1994
Authors :
Shaaya, Eli
;
.
Volume :
203
Co-Authors:
Zimowska, G., Insect Attractants, Behavior, and Basic Biology Research Laboratory, Agricultural Research Service, US Department of Agriculture, Gainesville, 32604, FL, United States, Department of Invertebrate Physiology, University of Warsaw, Warszawa, Poland
Shirk, P.D., Insect Attractants, Behavior, and Basic Biology Research Laboratory, Agricultural Research Service, US Department of Agriculture, Gainesville, 32604, FL, United States
Silhacek, D.L., Insect Attractants, Behavior, and Basic Biology Research Laboratory, Agricultural Research Service, US Department of Agriculture, Gainesville, 32604, FL, United States
Shaaya, E., The Volcani Center, Agricultural Research Organization, POB 6, Bet Dagan, 50-250, Israel
Facilitators :
From page:
215
To page:
226
(
Total pages:
12
)
Abstract:
We describe a provitellogenic stage, a previously unrecognized stage of follicle development in moths, and show that oocytes begin yolk sphere formation prior to the development of patency by the follicular epithelium. The vitellogenic activities of follicles from pharate adult female Plodia interpunctella (Hübner) were determined by visualizing the subunits of vitellin (YP1 and YP3) and the follicular epithelium yolk protein (YP2 and YP4) using monospecific antisera to each subunit to immunolabel whole-mounted ovaries or ultrathin sections. At 92 h after pupation, yolk spheres that contained only YP2 began to proliferate in the oocytes. The inter-follicular epithelial cell spaces were closed at 92 h making vitellogenin inaccessible to the oocyte, and consequently, the vitellin subunits were not observed in the yolk spheres. YP2 uptake most likely occurred across the brush border from the follicular epithelial cells to the oocyte at this time. At 105 h, the inter-follicular epithelial cell spaces appeared closed yet trace amounts of labeling for vitellin were observed in the spaces and also in the yolk spheres along with YP2. Equivalent labeling for all four YPs in yolk spheres was finally observed at 112 h after pupation when the follicular epithelium had become patent. These data indicate that the provitellogenic stage is an extended transition period between the previtellogenic and vitellogenic stages that lasts for approximately 13 h, and it is marked at the beginning by YP2 yolk sphere formation in the oocyte and at the end by patency in the follicular epithelium. © 1994 Springer-Verlag.
Note:
Related Files :
animal tissue
antibody labeling
brush border
epithelium cell
immunogold labeling
Lepidoptera
oocyte development
ovary
Show More
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More details
DOI :
10.1007/BF00636337
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19397
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:28
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Scientific Publication
Yolk sphere formation is initiated in oocytes before development of patency in follicles of the moth, Plodia interpunctella
203
Zimowska, G., Insect Attractants, Behavior, and Basic Biology Research Laboratory, Agricultural Research Service, US Department of Agriculture, Gainesville, 32604, FL, United States, Department of Invertebrate Physiology, University of Warsaw, Warszawa, Poland
Shirk, P.D., Insect Attractants, Behavior, and Basic Biology Research Laboratory, Agricultural Research Service, US Department of Agriculture, Gainesville, 32604, FL, United States
Silhacek, D.L., Insect Attractants, Behavior, and Basic Biology Research Laboratory, Agricultural Research Service, US Department of Agriculture, Gainesville, 32604, FL, United States
Shaaya, E., The Volcani Center, Agricultural Research Organization, POB 6, Bet Dagan, 50-250, Israel
Yolk sphere formation is initiated in oocytes before development of patency in follicles of the moth, Plodia interpunctella
We describe a provitellogenic stage, a previously unrecognized stage of follicle development in moths, and show that oocytes begin yolk sphere formation prior to the development of patency by the follicular epithelium. The vitellogenic activities of follicles from pharate adult female Plodia interpunctella (Hübner) were determined by visualizing the subunits of vitellin (YP1 and YP3) and the follicular epithelium yolk protein (YP2 and YP4) using monospecific antisera to each subunit to immunolabel whole-mounted ovaries or ultrathin sections. At 92 h after pupation, yolk spheres that contained only YP2 began to proliferate in the oocytes. The inter-follicular epithelial cell spaces were closed at 92 h making vitellogenin inaccessible to the oocyte, and consequently, the vitellin subunits were not observed in the yolk spheres. YP2 uptake most likely occurred across the brush border from the follicular epithelial cells to the oocyte at this time. At 105 h, the inter-follicular epithelial cell spaces appeared closed yet trace amounts of labeling for vitellin were observed in the spaces and also in the yolk spheres along with YP2. Equivalent labeling for all four YPs in yolk spheres was finally observed at 112 h after pupation when the follicular epithelium had become patent. These data indicate that the provitellogenic stage is an extended transition period between the previtellogenic and vitellogenic stages that lasts for approximately 13 h, and it is marked at the beginning by YP2 yolk sphere formation in the oocyte and at the end by patency in the follicular epithelium. © 1994 Springer-Verlag.
Scientific Publication
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