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New measures of insecticidal efficacy and safety obtained with the 39K promoter of a recombinant baculovirus
Year:
2006
Source of publication :
FEBS Letters
Authors :
Chejanovsky, Nor
;
.
Rivkin, Hadassah
;
.
Volume :
580
Co-Authors:
Regev, A., Entomology Department, Institute of Plant Protection, ARO, POB 6, Bet Dagan, 50250, Israel, Department of Plant Sciences, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, 69978, Israel
Rivkin, H., Entomology Department, Institute of Plant Protection, ARO, POB 6, Bet Dagan, 50250, Israel
Gurevitz, M., Department of Plant Sciences, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, 69978, Israel
Chejanovsky, N., Entomology Department, Institute of Plant Protection, ARO, POB 6, Bet Dagan, 50250, Israel
Facilitators :
From page:
6777
To page:
6782
(
Total pages:
6
)
Abstract:
Baculoviruses are orally infectious to insects and considered to be natural insecticides. To enhance their speed-of-kill these viruses were engineered to express arthropod neurotoxins under the control of various strong promoters. Although this strategy proved to be efficient, it raised recently concerns about safety. We analyzed the speed-of-kill and safety of Autographa californica multiple nucleopolyhedrovirus expressing the insecticidal scorpion neurotoxin AaIT and found that the mortality of Helicoverpa armigera larvae was enhanced significantly when the expression was controlled by the baculovirus delayed-early promoter 39K rather than the very late promoter p10. This improvement was also reflected in better protection of cotton leaves on which these insects were fed. Using lacZ as a sensitive reporter we also found that expression driven by the 39K promoter was detected in insect but not in mammalian cells. These results imply that by selection of an appropriate viral promoter, engineered baculoviruses may comply with the high standard biosafety requirements from a genetically modified organism (GMO). Our results provide further support for the potential use of engineered baculoviruses in insect pest control in a safely manner. © 2006 Federation of European Biochemical Societies.
Note:
Related Files :
Animals
gene expression
Gossypium
insecticides
Lepidoptera
Mammalia
Neurotoxin
Show More
Related Content
More details
DOI :
10.1016/j.febslet.2006.11.037
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19429
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:29
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Scientific Publication
New measures of insecticidal efficacy and safety obtained with the 39K promoter of a recombinant baculovirus
580
Regev, A., Entomology Department, Institute of Plant Protection, ARO, POB 6, Bet Dagan, 50250, Israel, Department of Plant Sciences, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, 69978, Israel
Rivkin, H., Entomology Department, Institute of Plant Protection, ARO, POB 6, Bet Dagan, 50250, Israel
Gurevitz, M., Department of Plant Sciences, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, 69978, Israel
Chejanovsky, N., Entomology Department, Institute of Plant Protection, ARO, POB 6, Bet Dagan, 50250, Israel
New measures of insecticidal efficacy and safety obtained with the 39K promoter of a recombinant baculovirus
Baculoviruses are orally infectious to insects and considered to be natural insecticides. To enhance their speed-of-kill these viruses were engineered to express arthropod neurotoxins under the control of various strong promoters. Although this strategy proved to be efficient, it raised recently concerns about safety. We analyzed the speed-of-kill and safety of Autographa californica multiple nucleopolyhedrovirus expressing the insecticidal scorpion neurotoxin AaIT and found that the mortality of Helicoverpa armigera larvae was enhanced significantly when the expression was controlled by the baculovirus delayed-early promoter 39K rather than the very late promoter p10. This improvement was also reflected in better protection of cotton leaves on which these insects were fed. Using lacZ as a sensitive reporter we also found that expression driven by the 39K promoter was detected in insect but not in mammalian cells. These results imply that by selection of an appropriate viral promoter, engineered baculoviruses may comply with the high standard biosafety requirements from a genetically modified organism (GMO). Our results provide further support for the potential use of engineered baculoviruses in insect pest control in a safely manner. © 2006 Federation of European Biochemical Societies.
Scientific Publication
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