נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Insertional inactivation of streptolysin S expression is associated with altered riboflavin metabolism in Streptococcus pyogenes
Year:
1997
Source of publication :
Microbial Pathogenesis
Authors :
Sela, Shlomo
;
.
Volume :
22
Co-Authors:
Liu, S., Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, 69978, Israel, VA medical centre, Research Service, 1030 Jefferson Avenue, Memphis, TN 3814, United States
Sela, S., Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, 69978, Israel
Cohen, G., Dept. of Molec. Microbiol. and B., Faculty of Life Science, Tel-Aviv University, Tel-Aviv, 69978, Israel
Jadoun, J., Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, 69978, Israel
Cheung, A., Lab. of Bact. Pathogenesis and I., Rockefeller University, 1230 York Avenue, New York, NY 10021-6399, United States
Ofek, I., Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, 69978, Israel
Facilitators :
From page:
227
To page:
234
(
Total pages:
8
)
Abstract:
Transposon Tn916 mutagenesis was used to create a mutant of Streptococcus pyogenes M type 3, designated ISS417, in which the ability to produce streptolysin S (SLS) and several other exoproteins was impaired. Concomitantly, the mutant became dependent upon riboflavin for growth and was able to grow in Todd Hewitt broth (THB) when supplemented with riboflavin or viboflavin-rich yeast extract. The parent strain was apparently able to utilize THB-derived components as a substitute for riboflavin, while the mutant was not. Although the parent strain grew well in synthetic medium, it was unable to produce SLS, except when it was supplemented with a small amount of THB. Thus, a component of THB was able to 'trigger' SLS formation in the parent strain; The mutant grew well in this medium, but was unable to produce SLS even when it was supplemented with THB. Southern hybridization analysis revealed that the ISS417 mutant harbours a single transposon insertion in its chromosome. Phage transduction experiments showed that the riboflavin dependency and the inability to make SLS phenotypes are co-transducible. The pleotrophic properties of the ISS417 mutant differ from those reported for insertional inactivation of the mga locus which regulates production of a number of surface proteins in S. pyogenes and the sar locus which regulates production of a number of exoproteins in Staphylococcus aureus. In view of the possibility that there exist a genetic linkage between the riboflavin biosynthetic pathway and expression of the oxygen-stable SLS, we hypothesize that SLS has a role in the growth economy of S. pyogenes.
Note:
Related Files :
gene expression
Hemolysis
Mutagenesis
Mutagenesis, Insertional
phenotype
riboflavin
Transduction, Genetic
Show More
Related Content
More details
DOI :
10.1006/mpat.1996.0107
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19445
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:29
You may also be interested in
Scientific Publication
Insertional inactivation of streptolysin S expression is associated with altered riboflavin metabolism in Streptococcus pyogenes
22
Liu, S., Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, 69978, Israel, VA medical centre, Research Service, 1030 Jefferson Avenue, Memphis, TN 3814, United States
Sela, S., Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, 69978, Israel
Cohen, G., Dept. of Molec. Microbiol. and B., Faculty of Life Science, Tel-Aviv University, Tel-Aviv, 69978, Israel
Jadoun, J., Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, 69978, Israel
Cheung, A., Lab. of Bact. Pathogenesis and I., Rockefeller University, 1230 York Avenue, New York, NY 10021-6399, United States
Ofek, I., Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, 69978, Israel
Insertional inactivation of streptolysin S expression is associated with altered riboflavin metabolism in Streptococcus pyogenes
Transposon Tn916 mutagenesis was used to create a mutant of Streptococcus pyogenes M type 3, designated ISS417, in which the ability to produce streptolysin S (SLS) and several other exoproteins was impaired. Concomitantly, the mutant became dependent upon riboflavin for growth and was able to grow in Todd Hewitt broth (THB) when supplemented with riboflavin or viboflavin-rich yeast extract. The parent strain was apparently able to utilize THB-derived components as a substitute for riboflavin, while the mutant was not. Although the parent strain grew well in synthetic medium, it was unable to produce SLS, except when it was supplemented with a small amount of THB. Thus, a component of THB was able to 'trigger' SLS formation in the parent strain; The mutant grew well in this medium, but was unable to produce SLS even when it was supplemented with THB. Southern hybridization analysis revealed that the ISS417 mutant harbours a single transposon insertion in its chromosome. Phage transduction experiments showed that the riboflavin dependency and the inability to make SLS phenotypes are co-transducible. The pleotrophic properties of the ISS417 mutant differ from those reported for insertional inactivation of the mga locus which regulates production of a number of surface proteins in S. pyogenes and the sar locus which regulates production of a number of exoproteins in Staphylococcus aureus. In view of the possibility that there exist a genetic linkage between the riboflavin biosynthetic pathway and expression of the oxygen-stable SLS, we hypothesize that SLS has a role in the growth economy of S. pyogenes.
Scientific Publication
You may also be interested in