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Genetic variation and evolutionary forces shaping Cucumber vein yellowing virus populations: Risk of emergence of virulent isolates in Europe
Year:
2016
Source of publication :
Plant Pathology
Authors :
Lapidot, Moshe
;
.
Volume :
65
Co-Authors:
Velasco, L., Instituto Andaluz de Investigación y Formación Agraria (IFAPA), Málaga, Spain
Salem, N., Department of Plant Protection, Faculty of Agriculture, The University of Jordan, Amman, Jordan
Willemsen, A., Instituto de Biología Molecular y Celular de Plantas (CSIC-UP), Valencia, Spain
Lapidot, M., Department of Vegetable Research, Institute of Plant Sciences (Volcani Center), Bet Dagan, Israel
Mansour, A.N., Department of Plant Protection, Faculty of Agriculture, The University of Jordan, Amman, Jordan
Rubio, L., Instituto Valenciano de Investigaciones Agrarias (IVIA), Valencia, Spain, Euro-Mediterranean Institute of Science and Technology (I.E.ME.S.T), Palermo, Italy
Galipienso, L., Instituto Valenciano de Investigaciones Agrarias (IVIA), Valencia, Spain, Euro-Mediterranean Institute of Science and Technology (I.E.ME.S.T), Palermo, Italy
Facilitators :
From page:
847
To page:
856
(
Total pages:
10
)
Abstract:
The genetic variation and evolutionary mechanisms shaping Cucumber vein yellowing virus (CVYV) populations were investigated by analysis of nucleotide sequences coding for P1b, P1b/P3 and coat proteins (CP) from isolates collected in different countries. The complete genome sequence of isolate ISM from Israel was also determined and compared to those of isolates Jor from Jordan and ALM32 from Spain. This isolate had overall nucleotide identities of 94·23 and 94·96% with ALM32 and Jor, respectively. Nucleotide variation among isolates was not homogeneously distributed, with the 5' half of the genome being more variable than the 3' half. A Bayesian phylogenetic tree of the CP showed that CVYV isolates clustered into two main clades: isolates from the Middle East region (Lebanon, Israel and Jordan) clustered in both clades whereas the isolate from Tunisia clustered in clade I and the European isolates clustered as a homogeneous phylogroup in Clade II. A similar topology was observed for P1b but with incongruences with respect to the CP, suggesting genetic exchange among virus isolates, which were confirmed with recombination algorithms. The low genetic diversity within the European phylogroup with respect to the other isolates, neutralist tests and genetic differentiation analyses suggest that the Middle East region is the cradle of CVYV and that a unique virus introduction event occurred in Europe, where the virus spread rapidly. Taken together, these findings indicate a risk of emergence of virulent CVYV isolates in Europe either through migration from the Middle East or by genetic changes of the European isolates. © 2016 British Society for Plant Pathology..
Note:
Related Files :
Cucumber vein yellowing virus
CVYV
gene flow
Ipomovirus
phylogeny
Potyviridae
recombination
Show More
Related Content
More details
DOI :
10.1111/ppa.12465
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19457
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:29
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Scientific Publication
Genetic variation and evolutionary forces shaping Cucumber vein yellowing virus populations: Risk of emergence of virulent isolates in Europe
65
Velasco, L., Instituto Andaluz de Investigación y Formación Agraria (IFAPA), Málaga, Spain
Salem, N., Department of Plant Protection, Faculty of Agriculture, The University of Jordan, Amman, Jordan
Willemsen, A., Instituto de Biología Molecular y Celular de Plantas (CSIC-UP), Valencia, Spain
Lapidot, M., Department of Vegetable Research, Institute of Plant Sciences (Volcani Center), Bet Dagan, Israel
Mansour, A.N., Department of Plant Protection, Faculty of Agriculture, The University of Jordan, Amman, Jordan
Rubio, L., Instituto Valenciano de Investigaciones Agrarias (IVIA), Valencia, Spain, Euro-Mediterranean Institute of Science and Technology (I.E.ME.S.T), Palermo, Italy
Galipienso, L., Instituto Valenciano de Investigaciones Agrarias (IVIA), Valencia, Spain, Euro-Mediterranean Institute of Science and Technology (I.E.ME.S.T), Palermo, Italy
Genetic variation and evolutionary forces shaping Cucumber vein yellowing virus populations: Risk of emergence of virulent isolates in Europe
The genetic variation and evolutionary mechanisms shaping Cucumber vein yellowing virus (CVYV) populations were investigated by analysis of nucleotide sequences coding for P1b, P1b/P3 and coat proteins (CP) from isolates collected in different countries. The complete genome sequence of isolate ISM from Israel was also determined and compared to those of isolates Jor from Jordan and ALM32 from Spain. This isolate had overall nucleotide identities of 94·23 and 94·96% with ALM32 and Jor, respectively. Nucleotide variation among isolates was not homogeneously distributed, with the 5' half of the genome being more variable than the 3' half. A Bayesian phylogenetic tree of the CP showed that CVYV isolates clustered into two main clades: isolates from the Middle East region (Lebanon, Israel and Jordan) clustered in both clades whereas the isolate from Tunisia clustered in clade I and the European isolates clustered as a homogeneous phylogroup in Clade II. A similar topology was observed for P1b but with incongruences with respect to the CP, suggesting genetic exchange among virus isolates, which were confirmed with recombination algorithms. The low genetic diversity within the European phylogroup with respect to the other isolates, neutralist tests and genetic differentiation analyses suggest that the Middle East region is the cradle of CVYV and that a unique virus introduction event occurred in Europe, where the virus spread rapidly. Taken together, these findings indicate a risk of emergence of virulent CVYV isolates in Europe either through migration from the Middle East or by genetic changes of the European isolates. © 2016 British Society for Plant Pathology..
Scientific Publication
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