נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
The characterization of LeNUC1, a nuclease associated with leaf senescence of tomato
Year:
2001
Source of publication :
Physiologia Plantarum
Authors :
Burd, Shaul
;
.
Kaltchiski, Andrei
;
.
Lers, Amnon
;
.
Lomaniec, Ella
;
.
Volume :
112
Co-Authors:
Lers, A., Dept. Postharvest Sci. Fresh Prod., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Lomaniec, E., Dept. Postharvest Sci. Fresh Prod., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Burd, S., Dept. Postharvest Sci. Fresh Prod., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Khalchitski, A., Dept. Postharvest Sci. Fresh Prod., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
176
To page:
182
(
Total pages:
7
)
Abstract:
Induction of nuclease and RNase activities, together with decreases in nucleic acid content are considered to be characteristics of senescence in higher plants. However, little is known about the specific identities or functions of the enzymes involved or the mechanisms controlling their activation. Here we report the identification of a 41-kDa-tomato nuclease, LeNUC1, which is specifically induced during tomato leaf senescence but not in ripening fruits. LeNUC1 is a glycoprotein, which can degrade both RNA and DNA and has optimal activity at pH 7.5-8. EDTA inhibits the activity of LeNUC1, while the addition of Co2+ or Mn2+ can restore its activity in the presence of the chelating agent. Interestingly, the activity of LeNUC1 is also induced in young leaves upon treatment with ethylene, which is known to be a senescence-promoting hormone in tomato. Constitutive activity of a 39-kDa nuclease, LeNUC2, similar in its biochemical requirements to LeNUC1, was also detected. LeNUC2 is not induced by ethylene and does not seem to be glycosylated. Based on their characteristics, LeNUC1 and LeNUC2 can be classified as Nuclease I enzymes. LeNUC1 may be involved in nucleic acid metabolism during tomato leaf senescence.
Note:
Related Files :
cobalt
DNA
enzymes
Fruits
hormones
metabolism
RNA
senescence
Show More
Related Content
More details
DOI :
10.1034/j.1399-3054.2001.1120205.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19472
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:29
You may also be interested in
Scientific Publication
The characterization of LeNUC1, a nuclease associated with leaf senescence of tomato
112
Lers, A., Dept. Postharvest Sci. Fresh Prod., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Lomaniec, E., Dept. Postharvest Sci. Fresh Prod., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Burd, S., Dept. Postharvest Sci. Fresh Prod., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Khalchitski, A., Dept. Postharvest Sci. Fresh Prod., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
The characterization of LeNUC1, a nuclease associated with leaf senescence of tomato
Induction of nuclease and RNase activities, together with decreases in nucleic acid content are considered to be characteristics of senescence in higher plants. However, little is known about the specific identities or functions of the enzymes involved or the mechanisms controlling their activation. Here we report the identification of a 41-kDa-tomato nuclease, LeNUC1, which is specifically induced during tomato leaf senescence but not in ripening fruits. LeNUC1 is a glycoprotein, which can degrade both RNA and DNA and has optimal activity at pH 7.5-8. EDTA inhibits the activity of LeNUC1, while the addition of Co2+ or Mn2+ can restore its activity in the presence of the chelating agent. Interestingly, the activity of LeNUC1 is also induced in young leaves upon treatment with ethylene, which is known to be a senescence-promoting hormone in tomato. Constitutive activity of a 39-kDa nuclease, LeNUC2, similar in its biochemical requirements to LeNUC1, was also detected. LeNUC2 is not induced by ethylene and does not seem to be glycosylated. Based on their characteristics, LeNUC1 and LeNUC2 can be classified as Nuclease I enzymes. LeNUC1 may be involved in nucleic acid metabolism during tomato leaf senescence.
Scientific Publication
You may also be interested in