נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Direct identification of beta adrenergic receptors on isolated bovine parathyroid cells
Year:
1977
Source of publication :
Endocrinology
Authors :
Hurvitz, Shmuel (Animal science)
;
.
Volume :
100
Co-Authors:
Brown, E.M., Metab. Dis. Branch, Nat. Inst. Arthrit. Metab. Digest. Dis., NIH, Bethesda, Md. 20014, United States
Hurwitz, S., Metab. Dis. Branch, Nat. Inst. Arthrit. Metab. Digest. Dis., NIH, Bethesda, Md. 20014, United States
Woodard, C.J., Metab. Dis. Branch, Nat. Inst. Arthrit. Metab. Digest. Dis., NIH, Bethesda, Md. 20014, United States
Aurbach, G.D., Metab. Dis. Branch, Nat. Inst. Arthrit. Metab. Digest. Dis., NIH, Bethesda, Md. 20014, United States
Facilitators :
From page:
1703
To page:
1709
(
Total pages:
7
)
Abstract:
The radioiodinated β-blocker iodohydroxybenzylpindolol ([ 125I]HYP) has been used to identify directly and characterize β-adrenergic receptors in isolated bovine parathyroid cells. [ 125I]HYP was bound rapidly and reversibly to isolated bovine parathyroid cell membranes. Scatchard analysis revealed a single class of binding sites with high affinity (4x10 10M -1) and low capacity (0.7 pmol/mg). Saturation analysis of [ 125I]HYP binding to intact bovine parathyroid cells suggested a site with similar affinity on whole cells and with a binding capacity of 5000-10,000 sites/cell. True dissociation constants (K(d)'s) for β-adrenergic agonists and antagonists were in good agreement with activation constants (K(A)'s) and inhibition constants (K(i)'s) for effects on adenylate cyclase in membrane preparations. These constants also were in reasonable agreement with K(A)'s and K(i)'s previously shown for effects of agonists and antagonists on cAMP accumulation and PTH release in whole cells. This study shows by direct analysis that β-adrenergic receptors exist on isolated bovine parathyroid cells, and that there is close coupling between receptor binding, effects on cAMP and hormonal release. This represents still another system in which [ 125I]HYP has been successfully used to study β adrenergic receptors in membrane as well as intact cell preparations.
Note:
Related Files :
Animal
cattle
cell membrane
Cyclic AMP
histochemistry
iodohydroxybenzylpindolol i 125
Parathyroid Glands
propranolol
Show More
Related Content
More details
DOI :
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19492
Last updated date:
21/08/2022 07:45
Creation date:
16/04/2018 23:29
You may also be interested in
Scientific Publication
Direct identification of beta adrenergic receptors on isolated bovine parathyroid cells
100
Brown, E.M., Metab. Dis. Branch, Nat. Inst. Arthrit. Metab. Digest. Dis., NIH, Bethesda, Md. 20014, United States
Hurwitz, S., Metab. Dis. Branch, Nat. Inst. Arthrit. Metab. Digest. Dis., NIH, Bethesda, Md. 20014, United States
Woodard, C.J., Metab. Dis. Branch, Nat. Inst. Arthrit. Metab. Digest. Dis., NIH, Bethesda, Md. 20014, United States
Aurbach, G.D., Metab. Dis. Branch, Nat. Inst. Arthrit. Metab. Digest. Dis., NIH, Bethesda, Md. 20014, United States
Direct identification of beta adrenergic receptors on isolated bovine parathyroid cells
The radioiodinated β-blocker iodohydroxybenzylpindolol ([ 125I]HYP) has been used to identify directly and characterize β-adrenergic receptors in isolated bovine parathyroid cells. [ 125I]HYP was bound rapidly and reversibly to isolated bovine parathyroid cell membranes. Scatchard analysis revealed a single class of binding sites with high affinity (4x10 10M -1) and low capacity (0.7 pmol/mg). Saturation analysis of [ 125I]HYP binding to intact bovine parathyroid cells suggested a site with similar affinity on whole cells and with a binding capacity of 5000-10,000 sites/cell. True dissociation constants (K(d)'s) for β-adrenergic agonists and antagonists were in good agreement with activation constants (K(A)'s) and inhibition constants (K(i)'s) for effects on adenylate cyclase in membrane preparations. These constants also were in reasonable agreement with K(A)'s and K(i)'s previously shown for effects of agonists and antagonists on cAMP accumulation and PTH release in whole cells. This study shows by direct analysis that β-adrenergic receptors exist on isolated bovine parathyroid cells, and that there is close coupling between receptor binding, effects on cAMP and hormonal release. This represents still another system in which [ 125I]HYP has been successfully used to study β adrenergic receptors in membrane as well as intact cell preparations.
Scientific Publication
You may also be interested in