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Inhibitory effect of ACC deaminase-producing bacteria on crown gall formation in tomato plants infected by Agrobacterium tumefaciens or A. vitis
Year:
2010
Source of publication :
Plant Pathology
Authors :
Lurie, Susan
;
.
Volume :
59
Co-Authors:
Toklikishvili, N., Otto Warburg Center for Biotechnology in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel, Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia
Dandurishvili, N., Otto Warburg Center for Biotechnology in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel, Khanchaveli Institute of Plant Protection, Tbilisi, Georgia
Vainstein, A., Otto Warburg Center for Biotechnology in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Tediashvili, M., Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia
Giorgobiani, N., Khanchaveli Institute of Plant Protection, Tbilisi, Georgia
Lurie, S., Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Szegedi, E., Research Institute for Viticulture and Enology, Kecskemet, Hungary
Glick, B.R., Department of Biology, University of Waterloo, Waterloo, ON, Canada
Chernin, L., Otto Warburg Center for Biotechnology in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Facilitators :
From page:
1023
To page:
1030
(
Total pages:
8
)
Abstract:
This study showed that various rhizosphere bacteria producing the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase (ACCD), which can degrade ACC, the immediate precursor of ethylene in plants, and thereby lower plant ethylene levels, can act as promising biocontrol agents of pathogenic strains of Agrobacterium tumefaciens and A. vitis. Soaking the roots of tomato (Solanum lycopersicum) seedlings in a suspension of the ACCD-producing Pseudomonas putida UW4, Burkholderia phytofirmans PsJN or Azospirillum brasilense Cd1843 transformed by plasmid pRKTACC carrying the ACCD-encoding gene acdS from UW4, significantly reduced the development of tumours on tomato plants injected 4-5 days later with pathogenic Agrobacterium strains via wounds on the plant stem. The fresh mass of tumours formed by plants pretreated with ACCD-producing strains was typically four- to fivefold less than that of tumours formed on control plants inoculated only with a pathogenic Agrobacterium strain. Simultaneously, the level of ethylene evolution per amount of tumour mass on plants pretreated with ACCD-producing bacteria decreased four to eight times compared with that from tumours formed on control plants or plants pretreated with bacteria deficient in ACCD production. Moreover, transgenic tomato plants expressing a bacterial ACCD were found to be highly resistant to crown gall formation relative to the parental, non-transformed tomato plants. The results support the hypothesis that ethylene is a crucial factor in Agrobacterium tumour formation, and that ACCD-produced rhizosphere bacteria may protect plants infected by pathogenic Agrobacteria from crown gall disease. No claim to original US government works. Plant Pathology © 2010 BSPP.
Note:
Related Files :
Agrobacterium
ethylene
rhizosphere
Solanum
transgenic plants
Show More
Related Content
More details
DOI :
10.1111/j.1365-3059.2010.02326.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19504
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:29
Scientific Publication
Inhibitory effect of ACC deaminase-producing bacteria on crown gall formation in tomato plants infected by Agrobacterium tumefaciens or A. vitis
59
Toklikishvili, N., Otto Warburg Center for Biotechnology in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel, Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia
Dandurishvili, N., Otto Warburg Center for Biotechnology in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel, Khanchaveli Institute of Plant Protection, Tbilisi, Georgia
Vainstein, A., Otto Warburg Center for Biotechnology in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Tediashvili, M., Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia
Giorgobiani, N., Khanchaveli Institute of Plant Protection, Tbilisi, Georgia
Lurie, S., Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Szegedi, E., Research Institute for Viticulture and Enology, Kecskemet, Hungary
Glick, B.R., Department of Biology, University of Waterloo, Waterloo, ON, Canada
Chernin, L., Otto Warburg Center for Biotechnology in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Inhibitory effect of ACC deaminase-producing bacteria on crown gall formation in tomato plants infected by Agrobacterium tumefaciens or A. vitis
This study showed that various rhizosphere bacteria producing the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase (ACCD), which can degrade ACC, the immediate precursor of ethylene in plants, and thereby lower plant ethylene levels, can act as promising biocontrol agents of pathogenic strains of Agrobacterium tumefaciens and A. vitis. Soaking the roots of tomato (Solanum lycopersicum) seedlings in a suspension of the ACCD-producing Pseudomonas putida UW4, Burkholderia phytofirmans PsJN or Azospirillum brasilense Cd1843 transformed by plasmid pRKTACC carrying the ACCD-encoding gene acdS from UW4, significantly reduced the development of tumours on tomato plants injected 4-5 days later with pathogenic Agrobacterium strains via wounds on the plant stem. The fresh mass of tumours formed by plants pretreated with ACCD-producing strains was typically four- to fivefold less than that of tumours formed on control plants inoculated only with a pathogenic Agrobacterium strain. Simultaneously, the level of ethylene evolution per amount of tumour mass on plants pretreated with ACCD-producing bacteria decreased four to eight times compared with that from tumours formed on control plants or plants pretreated with bacteria deficient in ACCD production. Moreover, transgenic tomato plants expressing a bacterial ACCD were found to be highly resistant to crown gall formation relative to the parental, non-transformed tomato plants. The results support the hypothesis that ethylene is a crucial factor in Agrobacterium tumour formation, and that ACCD-produced rhizosphere bacteria may protect plants infected by pathogenic Agrobacteria from crown gall disease. No claim to original US government works. Plant Pathology © 2010 BSPP.
Scientific Publication
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