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Trace metabolic profiling and pathway analysis of clomazone using LC-MS-MS and high-resolution MS
Year:
2011
Source of publication :
LC GC Europe
Authors :
Yasuor, Hagai
;
.
Volume :
24
Co-Authors:
Zou, W., California State Department of Toxic Substances Control, Berkeley, CA, United States
Yasuor, H., Department of Plant Sciences, University of California, Davis, CA, United States
Fischer, A.J., Department of Plant Sciences, University of California, Davis, CA, United States
Tolstikov, V.V., UC Davis Genome Center, University of California, Davis, CA, United States
Facilitators :
From page:
1
To page:
5
(
Total pages:
5
)
Abstract:
Detection, analysis and characterization of low-abundant metabolites remain an unresolved problem in metabolic studies. In this study, we report a novel approach to address this challenge. The current methodology is derived from the predictive multiple reaction monitoring (pMRM) mode available on triple-quadrupole linear ion trap mass spectrometry (MS) systems. The pMRM mode offers the highest sensitivity among various acquisition modes for studying trace levels of metabolites of the herbicide clomazone in plants. Additionally, this method allows for the identification of positional isomers of metabolites. Unknown metabolites were further identified and validated by obtaining accurate masses and isotopic ratios using selected ion monitoring (SIM) and data-dependent tandem mass spectrometry (MS-MS) scans on high-resolution liquid chromatography mass spectrometry (LC-MS). During structural assignment of its metabolites, the unique chlorine isotopic signature of clomazone was used as a naturally occurring label to study MS-MS fragmentations. © 2012 Advanstar Communications, Inc.
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DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19562
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:30
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Scientific Publication
Trace metabolic profiling and pathway analysis of clomazone using LC-MS-MS and high-resolution MS
24
Zou, W., California State Department of Toxic Substances Control, Berkeley, CA, United States
Yasuor, H., Department of Plant Sciences, University of California, Davis, CA, United States
Fischer, A.J., Department of Plant Sciences, University of California, Davis, CA, United States
Tolstikov, V.V., UC Davis Genome Center, University of California, Davis, CA, United States
Trace metabolic profiling and pathway analysis of clomazone using LC-MS-MS and high-resolution MS
Detection, analysis and characterization of low-abundant metabolites remain an unresolved problem in metabolic studies. In this study, we report a novel approach to address this challenge. The current methodology is derived from the predictive multiple reaction monitoring (pMRM) mode available on triple-quadrupole linear ion trap mass spectrometry (MS) systems. The pMRM mode offers the highest sensitivity among various acquisition modes for studying trace levels of metabolites of the herbicide clomazone in plants. Additionally, this method allows for the identification of positional isomers of metabolites. Unknown metabolites were further identified and validated by obtaining accurate masses and isotopic ratios using selected ion monitoring (SIM) and data-dependent tandem mass spectrometry (MS-MS) scans on high-resolution liquid chromatography mass spectrometry (LC-MS). During structural assignment of its metabolites, the unique chlorine isotopic signature of clomazone was used as a naturally occurring label to study MS-MS fragmentations. © 2012 Advanstar Communications, Inc.
Scientific Publication
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