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Bacterial outer membrane vesicles induce plant immune responses
Year:
2016
Authors :
Bahar, Ofir
;
.
Volume :
29
Co-Authors:
Bahar, O., Department of Plant Pathology and Weed Science, Agricultural Research Organization, Volcani Center, POB 6, Bet-Dagan, Israel
Mordukhovich, G., Department of Plant Pathology and Weed Science, Agricultural Research Organization, Volcani Center, POB 6, Bet-Dagan, Israel, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Luu, D.D., Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States
Schwessinger, B., Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States, Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States, Australian National University, Research School of Biology, Acton, ACT, Australia
Daudi, A., Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States, Bio-protocol, P.O. Box 61231, Palo Alto, CA, United States
Jehle, A.K., University Tübingen, Tübingen, Germany, Christian Doppler Laboratory for Monitoring of Microbial Contaminants, Institute for Milk Hygiene, Milk Technology and Food Science, University of Veterinary Medicine, Vienna, Austria
Felix, G., University Tübingen, Tübingen, Germany
Ronald, P.C., Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States, Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States
Facilitators :
From page:
374
To page:
384
(
Total pages:
11
)
Abstract:
Gram-negative bacteria continuously pinch off portions of their outer membrane, releasing membrane vesicles. These outer membrane vesicles (OMVs) are involved in multiple processes including cell-to-cell communication, biofilm formation, stress tolerance, horizontal gene transfer, and virulence. OMVs are also known modulators of the mammalian immune response. Despite the well-documented role of OMVs in mammalian-bacterial communication, their interaction with plants is not well studied. To examine whether OMVs of plant pathogens modulate the plant immune response, we purified OMVs from four different plant pathogens and used them to treat Arabidopsis thaliana. OMVs rapidly induced a reactive oxygen species burst, medium alkalinization, and defense gene expression in A. thaliana leaf discs, cell cultures, and seedlings, respectively. Western blot analysis revealed that EF-Tu is present in OMVs and that it serves as an elicitor of the plant immune response in this form. Our results further show that the immune coreceptors BAK1 and SOBIR1mediate OMVperception and response. Taken together, our results demonstrate that plants can detect and respond to OMV-associatedmolecules by activation of their immune system, revealing a new facet of plant-bacterial interactions. © 2016 The American Phytopathological Society.
Note:
Related Files :
arabidopsis
bacteria
cell membrane
Genetics
metabolism
Plant Disease
Plant Diseases
Show More
Related Content
More details
DOI :
10.1094/MPMI-12-15-0270-R
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19570
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:30
Scientific Publication
Bacterial outer membrane vesicles induce plant immune responses
29
Bahar, O., Department of Plant Pathology and Weed Science, Agricultural Research Organization, Volcani Center, POB 6, Bet-Dagan, Israel
Mordukhovich, G., Department of Plant Pathology and Weed Science, Agricultural Research Organization, Volcani Center, POB 6, Bet-Dagan, Israel, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Luu, D.D., Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States
Schwessinger, B., Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States, Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States, Australian National University, Research School of Biology, Acton, ACT, Australia
Daudi, A., Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States, Bio-protocol, P.O. Box 61231, Palo Alto, CA, United States
Jehle, A.K., University Tübingen, Tübingen, Germany, Christian Doppler Laboratory for Monitoring of Microbial Contaminants, Institute for Milk Hygiene, Milk Technology and Food Science, University of Veterinary Medicine, Vienna, Austria
Felix, G., University Tübingen, Tübingen, Germany
Ronald, P.C., Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States, Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States
Bacterial outer membrane vesicles induce plant immune responses
Gram-negative bacteria continuously pinch off portions of their outer membrane, releasing membrane vesicles. These outer membrane vesicles (OMVs) are involved in multiple processes including cell-to-cell communication, biofilm formation, stress tolerance, horizontal gene transfer, and virulence. OMVs are also known modulators of the mammalian immune response. Despite the well-documented role of OMVs in mammalian-bacterial communication, their interaction with plants is not well studied. To examine whether OMVs of plant pathogens modulate the plant immune response, we purified OMVs from four different plant pathogens and used them to treat Arabidopsis thaliana. OMVs rapidly induced a reactive oxygen species burst, medium alkalinization, and defense gene expression in A. thaliana leaf discs, cell cultures, and seedlings, respectively. Western blot analysis revealed that EF-Tu is present in OMVs and that it serves as an elicitor of the plant immune response in this form. Our results further show that the immune coreceptors BAK1 and SOBIR1mediate OMVperception and response. Taken together, our results demonstrate that plants can detect and respond to OMV-associatedmolecules by activation of their immune system, revealing a new facet of plant-bacterial interactions. © 2016 The American Phytopathological Society.
Scientific Publication
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