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Identification of Salmonella enterica genes with a role in persistence on lettuce leaves during cold storage by recombinase-based in vivo expression technology
Year:
2013
Source of publication :
Phytopathology
Authors :
Belausov, Eduard
;
.
Kroupitski, Yulia
;
.
Pinto, Rivka
;
.
Sela, Shlomo
;
.
Volume :
103
Co-Authors:
Kroupitski, Y., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest and Food Sciences, Rehovot, Israel, Department of Biochemistry and Food Science, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot, Israel
Brandl, M.T., Produce Safety and Microbiology Research Unit, United States Department of Agriculture-Agriculture Research Service, Albany, CA, United States
Pinto, R., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest and Food Sciences, Rehovot, Israel
Belausov, E., Confocal Microscopy Unit, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Tamir-Ariel, D., Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Burdman, S., Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Sela, S., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest and Food Sciences, Rehovot, Israel
Facilitators :
From page:
362
To page:
372
(
Total pages:
11
)
Abstract:
Recurrent outbreaks of enteric illness linked to lettuce and a lack of efficacious strategies to decontaminate produce underscores the need for a better understanding of the molecular interactions of foodborne pathogens with plants. This study aimed at identifying Salmonella enterica genes involved in the persistence of this organism on postharvest lettuce during cold storage using recombinase-based in vivo expression technology (RIVET). In total, 37 potentially induced loci were identified in four distinct screenings. Knockout mutations in eight upregulated genes revealed that four of them have a role in persistence of the pathogen in this system. These genes included stfC, bcsA, misL, and yidR, encoding a fimbrial outer membrane usher, a cellulose synthase catalytic subunit, an adhesin of the autotransporter family expressed from the Salmonella pathogenicity island-3, and a putative ATP/GTP-binding protein, respectively. bcsA, misL, and yidR but not stfC mutants were impaired also in attachment and biofilm formation, suggesting that these functions are required for survival of S. enterica on post-harvest lettuce. This is the first report that MisL, which has a role in Salmonella binding to fibronectin in animal hosts, is involved also in adhesion to plant tissue. Hence, our study uncovered a new plant attachment factor in Salmonella and demonstrates that RIVET is an effective approach for investigating human pathogen-plant interactions in a post-harvest leafy vegetable. © 2013 The American Phytopathological Society.
Note:
Related Files :
food storage
freezing
gene expression
Genetics
mutation
Salmonella
vegetables
Show More
Related Content
More details
DOI :
10.1094/PHYTO-10-12-0254-FI
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19577
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:30
Scientific Publication
Identification of Salmonella enterica genes with a role in persistence on lettuce leaves during cold storage by recombinase-based in vivo expression technology
103
Kroupitski, Y., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest and Food Sciences, Rehovot, Israel, Department of Biochemistry and Food Science, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot, Israel
Brandl, M.T., Produce Safety and Microbiology Research Unit, United States Department of Agriculture-Agriculture Research Service, Albany, CA, United States
Pinto, R., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest and Food Sciences, Rehovot, Israel
Belausov, E., Confocal Microscopy Unit, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Tamir-Ariel, D., Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Burdman, S., Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Sela, S., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest and Food Sciences, Rehovot, Israel
Identification of Salmonella enterica genes with a role in persistence on lettuce leaves during cold storage by recombinase-based in vivo expression technology
Recurrent outbreaks of enteric illness linked to lettuce and a lack of efficacious strategies to decontaminate produce underscores the need for a better understanding of the molecular interactions of foodborne pathogens with plants. This study aimed at identifying Salmonella enterica genes involved in the persistence of this organism on postharvest lettuce during cold storage using recombinase-based in vivo expression technology (RIVET). In total, 37 potentially induced loci were identified in four distinct screenings. Knockout mutations in eight upregulated genes revealed that four of them have a role in persistence of the pathogen in this system. These genes included stfC, bcsA, misL, and yidR, encoding a fimbrial outer membrane usher, a cellulose synthase catalytic subunit, an adhesin of the autotransporter family expressed from the Salmonella pathogenicity island-3, and a putative ATP/GTP-binding protein, respectively. bcsA, misL, and yidR but not stfC mutants were impaired also in attachment and biofilm formation, suggesting that these functions are required for survival of S. enterica on post-harvest lettuce. This is the first report that MisL, which has a role in Salmonella binding to fibronectin in animal hosts, is involved also in adhesion to plant tissue. Hence, our study uncovered a new plant attachment factor in Salmonella and demonstrates that RIVET is an effective approach for investigating human pathogen-plant interactions in a post-harvest leafy vegetable. © 2013 The American Phytopathological Society.
Scientific Publication
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