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PBAN receptor: Employment of anti-receptor antibodies for its characterization and for development of a microplate binding assay
Year:
2009
Source of publication :
Journal of Insect Physiology
Authors :
Altstein, Miriam
;
.
Ben Yosef, Tal
;
.
Ben-Aziz, Orna
;
.
Bronshtein, Alisa
;
.
Davidovitch, Michael
;
.
Volume :
55
Co-Authors:

Ben Yosef, T., Department of Entomology, The Volcani Center, Hamabacim St. 6, Bet Dagan, 50250, Israel
Bronshtein, A., Department of Entomology, The Volcani Center, Hamabacim St. 6, Bet Dagan, 50250, Israel
Ben Aziz, O., Department of Entomology, The Volcani Center, Hamabacim St. 6, Bet Dagan, 50250, Israel
Davidovitch, M., Department of Entomology, The Volcani Center, Hamabacim St. 6, Bet Dagan, 50250, Israel
Tirosh, I.
Altstein, M., Department of Entomology, The Volcani Center, Hamabacim St. 6, Bet Dagan, 50250, Israel

Facilitators :
From page:
825
To page:
833
(
Total pages:
9
)
Abstract:
This study describes generation of an anti-PBAN receptor (PBAN-R) antiserum and its employment for the characterization of the PK/PBAN-R(s). The antiserum recognized, in a specific and dose-dependent manner, the presence of PBAN-R in pheromone gland membrane preparations of three female moths: Heliothis peltigera, Helicoverpa armigera and Spodoptera littoralis. It also reacted specifically with the S. littoralis larval receptor in vivo, most likely by competing with the ligand on the binding site and consequently inhibiting cuticular melanization. Despite its ability to react with the receptor of H. peltigera in dot blot experiments, the antiserum did not react with the receptor in vivo and failed to inhibit sex pheromone biosynthesis. The antiserum was also used to develop two microplate binding assays. The Ab described in this study is the first raised against an insect neuropeptide (Np) receptor to be used in vivo, and its employment for characterization of the PK/PBAN-R(s) may thus provide important information on the mode of action of this Np family. The present study adds important information on the difference between the receptors in the two moth species, hints at the possible existence of receptor subtypes, and provides a platform for the development of a high-throughput assay (HTA) for screening of PK/PBAN agonists and antagonists. © 2009 Elsevier Ltd. All rights reserved.
Note:
Related Files :
Animal
Animals
chemistry
Female
Lepidoptera
metabolism
Moths
Peltigera
Rabbits
Show More
Related Content
More details
DOI :
10.1016/j.jinsphys.2009.05.006
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19611
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:30
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PBAN receptor: Employment of anti-receptor antibodies for its characterization and for development of a microplate binding assay
55

Ben Yosef, T., Department of Entomology, The Volcani Center, Hamabacim St. 6, Bet Dagan, 50250, Israel
Bronshtein, A., Department of Entomology, The Volcani Center, Hamabacim St. 6, Bet Dagan, 50250, Israel
Ben Aziz, O., Department of Entomology, The Volcani Center, Hamabacim St. 6, Bet Dagan, 50250, Israel
Davidovitch, M., Department of Entomology, The Volcani Center, Hamabacim St. 6, Bet Dagan, 50250, Israel
Tirosh, I.
Altstein, M., Department of Entomology, The Volcani Center, Hamabacim St. 6, Bet Dagan, 50250, Israel

PBAN receptor: Employment of anti-receptor antibodies for its characterization and for development of a microplate binding assay
This study describes generation of an anti-PBAN receptor (PBAN-R) antiserum and its employment for the characterization of the PK/PBAN-R(s). The antiserum recognized, in a specific and dose-dependent manner, the presence of PBAN-R in pheromone gland membrane preparations of three female moths: Heliothis peltigera, Helicoverpa armigera and Spodoptera littoralis. It also reacted specifically with the S. littoralis larval receptor in vivo, most likely by competing with the ligand on the binding site and consequently inhibiting cuticular melanization. Despite its ability to react with the receptor of H. peltigera in dot blot experiments, the antiserum did not react with the receptor in vivo and failed to inhibit sex pheromone biosynthesis. The antiserum was also used to develop two microplate binding assays. The Ab described in this study is the first raised against an insect neuropeptide (Np) receptor to be used in vivo, and its employment for characterization of the PK/PBAN-R(s) may thus provide important information on the mode of action of this Np family. The present study adds important information on the difference between the receptors in the two moth species, hints at the possible existence of receptor subtypes, and provides a platform for the development of a high-throughput assay (HTA) for screening of PK/PBAN agonists and antagonists. © 2009 Elsevier Ltd. All rights reserved.
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