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Identification of mature peptides from pban and capa genes of the moths Heliothis peltigera and Spodoptera littoralis
Year:
2017
Source of publication :
peptides ( source)
Authors :
Altstein, Miriam
;
.
Davidovitch, Michael
;
.
Salts, Yuval
;
.
Volume :
94
Co-Authors:
Ragionieri, L., Department of Biology, Institute for Zoology, Functional Peptidomics Group, University of Cologne, Cologne, Germany
Özbagci, B., Department of Biology, Institute for Zoology, Functional Peptidomics Group, University of Cologne, Cologne, Germany
Neupert, S., Department of Biology, Institute for Zoology, Functional Peptidomics Group, University of Cologne, Cologne, Germany
Salts, Y., Department of Entomology, The Volcani Center, Bet Dagan, Israel
Davidovitch, M., Department of Entomology, The Volcani Center, Bet Dagan, Israel
Altstein, M., Department of Entomology, The Volcani Center, Bet Dagan, Israel
Predel, R., Department of Biology, Institute for Zoology, Functional Peptidomics Group, University of Cologne, Cologne, Germany
Facilitators :
From page:
1
To page:
9
(
Total pages:
9
)
Abstract:
By transcriptome analysis, we identified PBAN and CAPA precursors in the moths Spodoptera littoralis and Heliothis peltigera which are among the most damaging pests of agriculture in tropical and subtropical Africa as well as in Mediterranean countries. A combination of mass spectrometry and immunocytochemistry was used to identify mature peptides processed from these precursors and to reveal their spatial distribution in the CNS. We found that the sites of expression of pban genes, the structure of PBAN precursors and the processed neuropeptides are very similar in noctuid moths. The sequence of the diapause hormone (DH; tryptopyrokinin following the signal peptide), however, contains two N-terminal amino acids more than expected from comparison with already published sequences of related species. Capa genes of S. littoralis and H. peltigera encode, in addition to periviscerokinins, a tryptopyrokinin showing sequence similarity with DH, which is the tryptopyrokinin of the pban gene. CAPA peptides, which were not known from any noctuid moth so far, are produced in cells of abdominal ganglia. The shape of the release sites of these hormones in H. peltigera represents an exceptionally derived trait state and does not resemble the well-structured abdominal perisympathetic organs which are known from many other insects. Instead, axons of CAPA cells extensively ramify within the ventral diaphragm. The novel information regarding the sequences of all mature peptides derived from pban and capa genes of H. peltigera and S. littoralis now enables a detailed analysis of the bioactivity and species-specificity of the native peptides, especially those from the hitherto unknown capa genes, and to explore their interactions with PBAN/DH receptors. © 2017 Elsevier Inc.
Note:
Related Files :
adult
Female
ganglion
Gene
gene expression
gene structure
Heliothis peltigera
Male
neuropeptide
pban gene
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Related Content
More details
DOI :
10.1016/j.peptides.2017.05.004
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19660
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:30
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Scientific Publication
Identification of mature peptides from pban and capa genes of the moths Heliothis peltigera and Spodoptera littoralis
94
Ragionieri, L., Department of Biology, Institute for Zoology, Functional Peptidomics Group, University of Cologne, Cologne, Germany
Özbagci, B., Department of Biology, Institute for Zoology, Functional Peptidomics Group, University of Cologne, Cologne, Germany
Neupert, S., Department of Biology, Institute for Zoology, Functional Peptidomics Group, University of Cologne, Cologne, Germany
Salts, Y., Department of Entomology, The Volcani Center, Bet Dagan, Israel
Davidovitch, M., Department of Entomology, The Volcani Center, Bet Dagan, Israel
Altstein, M., Department of Entomology, The Volcani Center, Bet Dagan, Israel
Predel, R., Department of Biology, Institute for Zoology, Functional Peptidomics Group, University of Cologne, Cologne, Germany
Identification of mature peptides from pban and capa genes of the moths Heliothis peltigera and Spodoptera littoralis
By transcriptome analysis, we identified PBAN and CAPA precursors in the moths Spodoptera littoralis and Heliothis peltigera which are among the most damaging pests of agriculture in tropical and subtropical Africa as well as in Mediterranean countries. A combination of mass spectrometry and immunocytochemistry was used to identify mature peptides processed from these precursors and to reveal their spatial distribution in the CNS. We found that the sites of expression of pban genes, the structure of PBAN precursors and the processed neuropeptides are very similar in noctuid moths. The sequence of the diapause hormone (DH; tryptopyrokinin following the signal peptide), however, contains two N-terminal amino acids more than expected from comparison with already published sequences of related species. Capa genes of S. littoralis and H. peltigera encode, in addition to periviscerokinins, a tryptopyrokinin showing sequence similarity with DH, which is the tryptopyrokinin of the pban gene. CAPA peptides, which were not known from any noctuid moth so far, are produced in cells of abdominal ganglia. The shape of the release sites of these hormones in H. peltigera represents an exceptionally derived trait state and does not resemble the well-structured abdominal perisympathetic organs which are known from many other insects. Instead, axons of CAPA cells extensively ramify within the ventral diaphragm. The novel information regarding the sequences of all mature peptides derived from pban and capa genes of H. peltigera and S. littoralis now enables a detailed analysis of the bioactivity and species-specificity of the native peptides, especially those from the hitherto unknown capa genes, and to explore their interactions with PBAN/DH receptors. © 2017 Elsevier Inc.
Scientific Publication
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