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Resistant ticks inhibit Metarhizium infection prior to haemocoel invasion by reducing fungal viability on the cuticle surface
Year:
2012
Source of publication :
Environmental Microbiology
Authors :
Belausov, Eduard
;
.
Gindin, Galina
;
.
Glazer, Itamar
;
.
Ment, Dana
;
.
Samish, Michael
;
.
Volume :
14
Co-Authors:
Ment, D., ARO, Volcani Center, Bet-Dagan, 50250, Israel, Robert H. Smith Faculty of Agricultural Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot, 76100, Israel
Churchill, A.C.L., Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, United States
Gindin, G., ARO, Volcani Center, Bet-Dagan, 50250, Israel
Belausov, E., ARO, Volcani Center, Bet-Dagan, 50250, Israel
Glazer, I., ARO, Volcani Center, Bet-Dagan, 50250, Israel
Rehner, S.A., USDA-ARS, Systematic Mycology and Microbiology Laboratory, Beltsville, MD 20705, United States
Rot, A., ARO, Kimron Veterinary Institute, Bet-Dagan, 50250, Israel
Donzelli, B.G.G., Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, United States
Samish, M., ARO, Volcani Center, Bet-Dagan, 50250, Israel
Facilitators :
From page:
1570
To page:
1583
(
Total pages:
14
)
Abstract:
We studied disease progression of, and host responses to, four species in the Metarhizium anisopliae complex expressing green fluorescent protein (GFP). We compared development and determined their relative levels of virulence against two susceptible arthropods, the cattle tick Rhipicephalus annulatus and the lepidopteran Galleria mellonella, and two resistant ticks, Hyalomma excavatum and Rhipicephalus sanguineus. Metarhizium brunneum Ma7 caused the greatest mortality of R.annulatus, Metarhizium robertsii ARSEF 2575 and Metarhizium pingshaense PPRC51 exhibited intermediate levels of virulence, and Metarhizium majus PPRC27 caused low mortality of cattle ticks. Conidia of all four species germinated on all hosts examined, but on resistant hosts, sustained hyphal growth was inhibited and GFP emission steadily and significantly decreased over time, suggesting a loss of fungal viability. Cuticle penetration was observed only for the three most virulent species infecting susceptible hosts. Cuticles of resistant and susceptible engorged female ticks showed significant increases in red autofluorescence at sites immediately under fungal hyphae. This is the first report (i) of tick mortality occurring after cuticle penetration but prior to haemocoel colonization and (ii) that resistant ticks do not support development of Metarhizium germlings on the outer surface of the cuticle. Whether reduced Metarhizium viability on resistant tick cuticles is due to antibiosis or limited nutrient availability is unknown. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.
Note:
Related Files :
Animal
Animals
biological control agent
cattle
Female
fungi
Growth, Development and Aging
Microbiology
Show More
Related Content
More details
DOI :
10.1111/j.1462-2920.2012.02747.x
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:

Itamar's own PDF.

ID:
19695
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:30
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Scientific Publication
Resistant ticks inhibit Metarhizium infection prior to haemocoel invasion by reducing fungal viability on the cuticle surface
14
Ment, D., ARO, Volcani Center, Bet-Dagan, 50250, Israel, Robert H. Smith Faculty of Agricultural Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot, 76100, Israel
Churchill, A.C.L., Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, United States
Gindin, G., ARO, Volcani Center, Bet-Dagan, 50250, Israel
Belausov, E., ARO, Volcani Center, Bet-Dagan, 50250, Israel
Glazer, I., ARO, Volcani Center, Bet-Dagan, 50250, Israel
Rehner, S.A., USDA-ARS, Systematic Mycology and Microbiology Laboratory, Beltsville, MD 20705, United States
Rot, A., ARO, Kimron Veterinary Institute, Bet-Dagan, 50250, Israel
Donzelli, B.G.G., Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, United States
Samish, M., ARO, Volcani Center, Bet-Dagan, 50250, Israel
Resistant ticks inhibit Metarhizium infection prior to haemocoel invasion by reducing fungal viability on the cuticle surface
We studied disease progression of, and host responses to, four species in the Metarhizium anisopliae complex expressing green fluorescent protein (GFP). We compared development and determined their relative levels of virulence against two susceptible arthropods, the cattle tick Rhipicephalus annulatus and the lepidopteran Galleria mellonella, and two resistant ticks, Hyalomma excavatum and Rhipicephalus sanguineus. Metarhizium brunneum Ma7 caused the greatest mortality of R.annulatus, Metarhizium robertsii ARSEF 2575 and Metarhizium pingshaense PPRC51 exhibited intermediate levels of virulence, and Metarhizium majus PPRC27 caused low mortality of cattle ticks. Conidia of all four species germinated on all hosts examined, but on resistant hosts, sustained hyphal growth was inhibited and GFP emission steadily and significantly decreased over time, suggesting a loss of fungal viability. Cuticle penetration was observed only for the three most virulent species infecting susceptible hosts. Cuticles of resistant and susceptible engorged female ticks showed significant increases in red autofluorescence at sites immediately under fungal hyphae. This is the first report (i) of tick mortality occurring after cuticle penetration but prior to haemocoel colonization and (ii) that resistant ticks do not support development of Metarhizium germlings on the outer surface of the cuticle. Whether reduced Metarhizium viability on resistant tick cuticles is due to antibiosis or limited nutrient availability is unknown. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.
Scientific Publication
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