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Mechanisms and improved biocontrol of the root-knot nematodes by Trichoderma spp.
Year:
2005
Source of publication :
Acta Horticulturae
Authors :
Sharon, Edna
;
.
Spiegel, Yitzhak
;
.
Volume :
698
Co-Authors:
Spiegel, Y., Division of Nematology, ARO, Volcani Center, Bet Dagan 50250, Israel
Sharon, E., Division of Nematology, ARO, Volcani Center, Bet Dagan 50250, Israel
Chet, I., Department of Biological Chemistry, Weizmann Institute of Science, Israel
Facilitators :
From page:
225
To page:
228
(
Total pages:
4
)
Abstract:
Comprehensive studies in our laboratory revealed the nematicidal potential of waste products from crustacean shells, or other proteinaceous compounds, for controlling species of several plant-parasitic nematodes. The bacteria Telluria chitinolytica sp. nov, and Bacillus cereus, which were isolated, grown and identified in our laboratory, appeared to have potential as bioagents to control the root-knot nematode Meloidogyne javanica. Intensive studies of the mode of action of these bacteria were carried out. The fungus Trichoderma exhibits biocontrol activity against the root-knot nematode M. javanica. Direct fungal parasitism is one of various possible mechanisms by which the fungus can act against the nematode. A constitutive GFP (green fluorescent protein)- transformant of T. asperellum was used to observe the fungal parasitism on nematode eggs and juveniles, using laser scanning confocal microscopy. The involvement of lytic enzymes in the process was demonstrated using inductive GFP-fungal transformants carrying a fusion of the proteinase or chitinase promoters with the gfp gene. These enzyme genes turned on during the interaction between the fungus and the nematodes. The direct fungal parasitism process on the nematode could be improved, in vitro, by using antibodies that bound to the surface of M. javanica J2 and eggs. The presence of these antibodies enhanced the attachment of the spores to the nematodes. This resulted in a significant enhancement of fungal parasitism on the nematodes.
Note:
Related Files :
biological control
biological control
Meloidogyne javanica
Nematoda
plant protection
Root-knot nematode
Trichoderma
Trichoderma spp
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More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19717
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:31
Scientific Publication
Mechanisms and improved biocontrol of the root-knot nematodes by Trichoderma spp.
698
Spiegel, Y., Division of Nematology, ARO, Volcani Center, Bet Dagan 50250, Israel
Sharon, E., Division of Nematology, ARO, Volcani Center, Bet Dagan 50250, Israel
Chet, I., Department of Biological Chemistry, Weizmann Institute of Science, Israel
Mechanisms and improved biocontrol of the root-knot nematodes by Trichoderma spp.
Comprehensive studies in our laboratory revealed the nematicidal potential of waste products from crustacean shells, or other proteinaceous compounds, for controlling species of several plant-parasitic nematodes. The bacteria Telluria chitinolytica sp. nov, and Bacillus cereus, which were isolated, grown and identified in our laboratory, appeared to have potential as bioagents to control the root-knot nematode Meloidogyne javanica. Intensive studies of the mode of action of these bacteria were carried out. The fungus Trichoderma exhibits biocontrol activity against the root-knot nematode M. javanica. Direct fungal parasitism is one of various possible mechanisms by which the fungus can act against the nematode. A constitutive GFP (green fluorescent protein)- transformant of T. asperellum was used to observe the fungal parasitism on nematode eggs and juveniles, using laser scanning confocal microscopy. The involvement of lytic enzymes in the process was demonstrated using inductive GFP-fungal transformants carrying a fusion of the proteinase or chitinase promoters with the gfp gene. These enzyme genes turned on during the interaction between the fungus and the nematodes. The direct fungal parasitism process on the nematode could be improved, in vitro, by using antibodies that bound to the surface of M. javanica J2 and eggs. The presence of these antibodies enhanced the attachment of the spores to the nematodes. This resulted in a significant enhancement of fungal parasitism on the nematodes.
Scientific Publication
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