נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Survival of introduced Tuber melanosyorum at two sites in Israel as measured by occurrence of mycorrhizas
Year:
2001
Source of publication :
Plant and Soil
Authors :
Freeman, Stanley
;
.
Shabi, Ezra
;
.
Volume :
229
Co-Authors:
Kagan-Zur, V., Inst. Appl. Res., P.O. Box 653, Be'er Sheva, 84105, Israel
Freeman, S., ARO, Volcani Center, Dept. of Plant Pathology, Bet Dagan 50250, Israel
Luzzati, Y., Kibbutz bar'Am, M.P. Merom Hagalil 13860, United States
Roth-Bejerano, N., Life Sciences Dept., P.O. Box 653, Be'er Sheva, 84105, Israel
Shabi, E., Ben-Gurion University, Israel
Facilitators :
From page:
159
To page:
166
(
Total pages:
8
)
Abstract:
In the framework of efforts to introduce Tuber melanosporum as a cultivated crop to Israel, spores of the truffle, obtained from fruit-bodies procured in Italy and France, were used to inoculate oak seedlings and hazel suckers. Typical T. melanosporum mycorrhizas were observed 3 months after inoculation on roots of both plant species. One- to two-year-old mycorrhizal seedlings were outplanted at a number of experimental sites and irrigated regularly. Two sites characterized by alkaline soil but differing in soil composition and climatic conditions were chosen for the present study. DNA of ascocarps used for inoculation, DNA of re-isolated cultures and fungal DNA taken from tree mycorrhizas 4 years after outplanting were compared with T. melanosporum reference cultures by molecular methods. All T. melanosporum profiles proved to be identical except for one belonging to a reference culture, which exhibited an unusual HinfI ITS-RFLP pattern. A single base substitution, responsible for the different HinfI restriction site, distinguished the ITS region of this culture from a published T. melanosporum ITS sequence. ITS restriction polymorphism analyses determined that roots of all potted plants tested and many 4-year-old trees from the two experimental plots (irrespective of soil and climatic differences) were colonized by T. melanosporum.
Note:
Related Files :
Catostomidae
France
Inoculation
Israel
Italy
Mycorrhiza
mycorrhizae
Quercus
Show More
Related Content
More details
DOI :
10.1023/A:1004876504658
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19750
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:31
Scientific Publication
Survival of introduced Tuber melanosyorum at two sites in Israel as measured by occurrence of mycorrhizas
229
Kagan-Zur, V., Inst. Appl. Res., P.O. Box 653, Be'er Sheva, 84105, Israel
Freeman, S., ARO, Volcani Center, Dept. of Plant Pathology, Bet Dagan 50250, Israel
Luzzati, Y., Kibbutz bar'Am, M.P. Merom Hagalil 13860, United States
Roth-Bejerano, N., Life Sciences Dept., P.O. Box 653, Be'er Sheva, 84105, Israel
Shabi, E., Ben-Gurion University, Israel
Survival of introduced Tuber melanosyorum at two sites in Israel as measured by occurrence of mycorrhizas
In the framework of efforts to introduce Tuber melanosporum as a cultivated crop to Israel, spores of the truffle, obtained from fruit-bodies procured in Italy and France, were used to inoculate oak seedlings and hazel suckers. Typical T. melanosporum mycorrhizas were observed 3 months after inoculation on roots of both plant species. One- to two-year-old mycorrhizal seedlings were outplanted at a number of experimental sites and irrigated regularly. Two sites characterized by alkaline soil but differing in soil composition and climatic conditions were chosen for the present study. DNA of ascocarps used for inoculation, DNA of re-isolated cultures and fungal DNA taken from tree mycorrhizas 4 years after outplanting were compared with T. melanosporum reference cultures by molecular methods. All T. melanosporum profiles proved to be identical except for one belonging to a reference culture, which exhibited an unusual HinfI ITS-RFLP pattern. A single base substitution, responsible for the different HinfI restriction site, distinguished the ITS region of this culture from a published T. melanosporum ITS sequence. ITS restriction polymorphism analyses determined that roots of all potted plants tested and many 4-year-old trees from the two experimental plots (irrespective of soil and climatic differences) were colonized by T. melanosporum.
Scientific Publication
You may also be interested in