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Animal Biotechnology
Gootwine, E., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50250, Israel
Ofir, R., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50250, Israel
Yossefi, S., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50250, Israel
The GH2 allele of the ovine growth hormone (GH) locus contains two GH gene copies, GH2-N and GH2-Z. We describe a GH2 allele in which the two gene copies are polymorphic for PvuII sites at the second exon and at the second intron. PvuII-restriction analysis of pituitary-derived cDNA of a non-lactating ewe, homozygous for that GH2 allele with the 1.0+1.3+3.5 kb PvuII restriction pattern, suggests that in that ewe, only the GH2-N gene copy was expressed.
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Characterization of PvuII polymorphisms between the ovine growth hormone GH2-N and GH2-Z gene copies
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Gootwine, E., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50250, Israel
Ofir, R., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50250, Israel
Yossefi, S., Institute of Animal Science, A.R.O., Volcani Center, POB 6, Bet Dagan 50250, Israel
Characterization of PvuII polymorphisms between the ovine growth hormone GH2-N and GH2-Z gene copies
The GH2 allele of the ovine growth hormone (GH) locus contains two GH gene copies, GH2-N and GH2-Z. We describe a GH2 allele in which the two gene copies are polymorphic for PvuII sites at the second exon and at the second intron. PvuII-restriction analysis of pituitary-derived cDNA of a non-lactating ewe, homozygous for that GH2 allele with the 1.0+1.3+3.5 kb PvuII restriction pattern, suggests that in that ewe, only the GH2-N gene copy was expressed.
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