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Posttranslational ruling of xanthine oxidase activity in bovine milk by its substrates
Year:
2007
Authors :
Shapiro, Fira
;
.
Silanikove, Nissim
;
.
Volume :
363
Co-Authors:
Silanikove, N., Ruminant Physiology, Institute of Animal Science, Agricultural Research Organization, P.O. Box 6, Bet Dagan, 50250, Israel
Shapiro, F., Ruminant Physiology, Institute of Animal Science, Agricultural Research Organization, P.O. Box 6, Bet Dagan, 50250, Israel
Leitner, G., National Mastitis Reference Center, Kimron Veterinary Institute, Bet Dagan, 50250, Israel
Facilitators :
From page:
561
To page:
565
(
Total pages:
5
)
Abstract:
The aims of this study were to test the hypothesis that the substrates of xanthine oxidase (XO), xanthine and hypoxanthine, are consumed while the milk is stored in the gland between milkings, and to explore how XO activity responds to bacteria commonly associated with subclinical infections in the mammary gland. Freshly secreted milk was obtained following complete evacuation of the gland and induction of milk ejection with oxytocin. In bacteria-free fresh milk xanthine and hypoxanthine were converted to uric acid within 30 min (T1/2 ∼ 10 min), which in turn provides electrons for formation of hydrogen peroxide and endows the alveolar lumen with passive protection against invading bacteria. On the other hand, the longer residence time of milk in the cistern compartment was not associated with oxidative stress as a result of XO idleness caused by exhaustion of its physiological fuels. The specific response of XO to bacteria species and the resulting bacteria-dependent nitrosative stress further demonstrates that it is part of the gland immune system. © 2007 Elsevier Inc. All rights reserved.
Note:
Related Files :
Animals
cattle
enzyme activity
Female
lactation
Mammary gland
milk
Nitrosative stress
Storage
Show More
Related Content
More details
DOI :
10.1016/j.bbrc.2007.08.188
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19782
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:31
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Scientific Publication
Posttranslational ruling of xanthine oxidase activity in bovine milk by its substrates
363
Silanikove, N., Ruminant Physiology, Institute of Animal Science, Agricultural Research Organization, P.O. Box 6, Bet Dagan, 50250, Israel
Shapiro, F., Ruminant Physiology, Institute of Animal Science, Agricultural Research Organization, P.O. Box 6, Bet Dagan, 50250, Israel
Leitner, G., National Mastitis Reference Center, Kimron Veterinary Institute, Bet Dagan, 50250, Israel
Posttranslational ruling of xanthine oxidase activity in bovine milk by its substrates
The aims of this study were to test the hypothesis that the substrates of xanthine oxidase (XO), xanthine and hypoxanthine, are consumed while the milk is stored in the gland between milkings, and to explore how XO activity responds to bacteria commonly associated with subclinical infections in the mammary gland. Freshly secreted milk was obtained following complete evacuation of the gland and induction of milk ejection with oxytocin. In bacteria-free fresh milk xanthine and hypoxanthine were converted to uric acid within 30 min (T1/2 ∼ 10 min), which in turn provides electrons for formation of hydrogen peroxide and endows the alveolar lumen with passive protection against invading bacteria. On the other hand, the longer residence time of milk in the cistern compartment was not associated with oxidative stress as a result of XO idleness caused by exhaustion of its physiological fuels. The specific response of XO to bacteria species and the resulting bacteria-dependent nitrosative stress further demonstrates that it is part of the gland immune system. © 2007 Elsevier Inc. All rights reserved.
Scientific Publication
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