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Fluorescent in situ hybridization for the localization of viruses, bacteria and other microorganisms in insect and plant tissues -2016
Year:
2016
Source of publication :
Methods
Authors :
Ghanim, Murad
;
.
Kliot, Adi
;
.
Volume :
98
Co-Authors:
Kliot, A., Department of Entomology, The Volcani Center, Bet Dagan, Israel
Ghanim, M., Department of Entomology, The Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
74
To page:
81
(
Total pages:
8
)
Abstract:
Methods for the localization of cellular components such as nucleic acids, proteins, cellular vesicles and more, and the localization of microorganisms including viruses, bacteria and fungi have become an important part of any research program in biological sciences that enable the visualization of these components in fixed and live tissues without the need for complex processing steps. The rapid development of microscopy tools and technologies as well as related fluorescent markers and fluorophores for many cellular components, and the ability to design DNA and RNA sequence-based molecular probes and antibodies which can be visualized fluorescently, have rapidly advanced this field. This review will focus on some of the localizations methods which have been used in plants and insect pests in agriculture, and other microorganisms, which are rapidly advancing the research in agriculture-related fields. © 2015 Elsevier Inc.
Note:
Related Files :
bacteria
Fluorescent in situ hybridization
Localization
plant virus
symbiont
Show More
Related Content
More details
DOI :
10.1016/j.ymeth.2015.12.003
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
Review
;
.
Language:
English
Editors' remarks:
ID:
19796
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:31
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Scientific Publication
Fluorescent in situ hybridization for the localization of viruses, bacteria and other microorganisms in insect and plant tissues -2016
98
Kliot, A., Department of Entomology, The Volcani Center, Bet Dagan, Israel
Ghanim, M., Department of Entomology, The Volcani Center, Bet Dagan, Israel
Fluorescent in situ hybridization for the localization of viruses, bacteria and other microorganisms in insect and plant tissues
Methods for the localization of cellular components such as nucleic acids, proteins, cellular vesicles and more, and the localization of microorganisms including viruses, bacteria and fungi have become an important part of any research program in biological sciences that enable the visualization of these components in fixed and live tissues without the need for complex processing steps. The rapid development of microscopy tools and technologies as well as related fluorescent markers and fluorophores for many cellular components, and the ability to design DNA and RNA sequence-based molecular probes and antibodies which can be visualized fluorescently, have rapidly advanced this field. This review will focus on some of the localizations methods which have been used in plants and insect pests in agriculture, and other microorganisms, which are rapidly advancing the research in agriculture-related fields. © 2015 Elsevier Inc.
Scientific Publication
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