נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Antioxidant and prooxidant effects of phenolics on pancreatic β-cells in vitro
Year:
2002
Authors :
Kanner, Joseph
;
.
Volume :
50
Co-Authors:
Lapidot, T., Department of Food Science, ARO Volcani Center, Bet Dagan 50250, Israel
Walker, M.D., Department of Food Science, ARO Volcani Center, Bet Dagan 50250, Israel
Kanner, J., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Facilitators :
From page:
7220
To page:
7225
(
Total pages:
6
)
Abstract:
A number of natural phenolic compounds display antioxidant and cell protective effects in cell culture models, yet in some studies show prooxidant and cytotoxic effects. Pancreatic β-cells have been reported to exhibit particular sensitivity to oxidative stress, a factor that may contribute to the impaired β-cell function characteristic of diabetes. The aim of this study was to examine the potential of natural phenolics to protect cultured pancreatic β-cells (βTC1 and HIT) from H 2O 2 oxidative stress. Exposure of cells to H 2O 2 led to significant proliferation inhibition. Contrary to what one should expect, simultaneous exposure to H 2O 2 and the phenolics, quercetin (10-100 μM), catechin (50-500 μM), or ascorbic acid (100-1000 μM), led to amplification of proliferation inhibition. At higher concentrations, these compounds inhibited proliferation, even in the absence of added H 2O 2. This prooxidant effect is attributable to the generation of H 2O 2 through interaction of the added phenolic compounds with as yet undefined componenets of the culture media. On the other hand, inclusion of metmyoglobin (30 μM) in the culture medium significantly reduced the prooxidant impact of the phenolics. Under these conditions, quercetin and catechin significantly protected the cells against oxidative stress when these components were present during the stress period. Furthermore, significant cell protection was observed upon preincubation of cells with chrysin, quercetin, catechin, or caffeic acid (50 μM, each) prior to application of oxidative stress. It is concluded that provided artifactual prooxidant effects are avoided, preincubation of β-cells with relatively hydrophobic natural phenolics can confer protection against oxidative stress.
Note:
Related Files :
Animal
Animals
antioxidants
Cell Proliferation
flavonoids
metabolism
mice
phenols
stress
Show More
Related Content
More details
DOI :
10.1021/jf020615a
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19814
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:31
You may also be interested in
Scientific Publication
Antioxidant and prooxidant effects of phenolics on pancreatic β-cells in vitro
50
Lapidot, T., Department of Food Science, ARO Volcani Center, Bet Dagan 50250, Israel
Walker, M.D., Department of Food Science, ARO Volcani Center, Bet Dagan 50250, Israel
Kanner, J., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Antioxidant and prooxidant effects of phenolics on pancreatic β-cells in vitro
A number of natural phenolic compounds display antioxidant and cell protective effects in cell culture models, yet in some studies show prooxidant and cytotoxic effects. Pancreatic β-cells have been reported to exhibit particular sensitivity to oxidative stress, a factor that may contribute to the impaired β-cell function characteristic of diabetes. The aim of this study was to examine the potential of natural phenolics to protect cultured pancreatic β-cells (βTC1 and HIT) from H 2O 2 oxidative stress. Exposure of cells to H 2O 2 led to significant proliferation inhibition. Contrary to what one should expect, simultaneous exposure to H 2O 2 and the phenolics, quercetin (10-100 μM), catechin (50-500 μM), or ascorbic acid (100-1000 μM), led to amplification of proliferation inhibition. At higher concentrations, these compounds inhibited proliferation, even in the absence of added H 2O 2. This prooxidant effect is attributable to the generation of H 2O 2 through interaction of the added phenolic compounds with as yet undefined componenets of the culture media. On the other hand, inclusion of metmyoglobin (30 μM) in the culture medium significantly reduced the prooxidant impact of the phenolics. Under these conditions, quercetin and catechin significantly protected the cells against oxidative stress when these components were present during the stress period. Furthermore, significant cell protection was observed upon preincubation of cells with chrysin, quercetin, catechin, or caffeic acid (50 μM, each) prior to application of oxidative stress. It is concluded that provided artifactual prooxidant effects are avoided, preincubation of β-cells with relatively hydrophobic natural phenolics can confer protection against oxidative stress.
Scientific Publication
You may also be interested in