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Efficiency of phenol biodegradation by planktonic Pseudomonas pseudoalcaligenes (a constructed wetland isolate) vs. root and gravel biofilm
Year:
2010
Source of publication :
Water Research
Authors :
Sela, Shlomo
;
.
Volume :
44
Co-Authors:
Kurzbaum, E., Faculty of Civil and Environmental Engineering, Division of Environmental, Water and Agricultural Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel
Kirzhner, F., Faculty of Civil and Environmental Engineering, Division of Environmental, Water and Agricultural Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel
Sela, S., Department of Food Science, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Zimmels, Y., Faculty of Civil and Environmental Engineering, Division of Environmental, Water and Agricultural Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel
Armon, R., Faculty of Civil and Environmental Engineering, Division of Environmental, Water and Agricultural Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel
Facilitators :
From page:
5021
To page:
5031
(
Total pages:
11
)
Abstract:
In the last two decades, constructed wetland systems gained increasing interest in wastewater treatment and as such have been intensively studied around the world. While most of the studies showed excellent removal of various pollutants, the exact contribution, in kinetic terms, of its particular components (such as: root, gravel and water) combined with bacteria is almost nonexistent.In the present study, a phenol degrader bacterium identified as Pseudomonas pseudoalcaligenes was isolated from a constructed wetland, and used in an experimental set-up containing: plants and gravel. Phenol removal rate by planktonic and biofilm bacteria (on sterile Zea mays roots and gravel surfaces) was studied. Specific phenol removal rates revealed significant advantage of planktonic cells (1.04 × 10 -9 mg phenol/CFU/h) compared to root and gravel biofilms: 4.59 × 10 -11-2.04 × 10 -10 and 8.04 × 10 -11-4.39 × 10 -10 (mg phenol/CFU/h), respectively.In batch cultures, phenol biodegradation kinetic parameters were determined by biomass growth rates and phenol removal as a function of time. Based on Haldane equation, kinetic constants such as μ max = 1.15/h, K s = 35.4 mg/L and K i = 198.6 mg/L fit well phenol removal by P. pseudoalcaligenes.Although P. pseudoalcaligenes planktonic cells showed the highest phenol removal rate, in constructed wetland systems and especially in those with sub-surface flow, it is expected that surface associated microorganisms (biofilms) will provide a much higher contribution in phenol and other organics removal, due to greater bacterial biomass.Factors affecting the performance of planktonic vs. biofilm bacteria in sub-surface flow constructed wetlands are further discussed. © 2010 Elsevier Ltd.
Note:
Related Files :
bacteria
Biodegradation
Biomass
phenols
rhizosphere
soil
wastewater treatment
Zea mays
Show More
Related Content
More details
DOI :
10.1016/j.watres.2010.07.020
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19876
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:32
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Scientific Publication
Efficiency of phenol biodegradation by planktonic Pseudomonas pseudoalcaligenes (a constructed wetland isolate) vs. root and gravel biofilm
44
Kurzbaum, E., Faculty of Civil and Environmental Engineering, Division of Environmental, Water and Agricultural Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel
Kirzhner, F., Faculty of Civil and Environmental Engineering, Division of Environmental, Water and Agricultural Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel
Sela, S., Department of Food Science, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Zimmels, Y., Faculty of Civil and Environmental Engineering, Division of Environmental, Water and Agricultural Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel
Armon, R., Faculty of Civil and Environmental Engineering, Division of Environmental, Water and Agricultural Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel
Efficiency of phenol biodegradation by planktonic Pseudomonas pseudoalcaligenes (a constructed wetland isolate) vs. root and gravel biofilm
In the last two decades, constructed wetland systems gained increasing interest in wastewater treatment and as such have been intensively studied around the world. While most of the studies showed excellent removal of various pollutants, the exact contribution, in kinetic terms, of its particular components (such as: root, gravel and water) combined with bacteria is almost nonexistent.In the present study, a phenol degrader bacterium identified as Pseudomonas pseudoalcaligenes was isolated from a constructed wetland, and used in an experimental set-up containing: plants and gravel. Phenol removal rate by planktonic and biofilm bacteria (on sterile Zea mays roots and gravel surfaces) was studied. Specific phenol removal rates revealed significant advantage of planktonic cells (1.04 × 10 -9 mg phenol/CFU/h) compared to root and gravel biofilms: 4.59 × 10 -11-2.04 × 10 -10 and 8.04 × 10 -11-4.39 × 10 -10 (mg phenol/CFU/h), respectively.In batch cultures, phenol biodegradation kinetic parameters were determined by biomass growth rates and phenol removal as a function of time. Based on Haldane equation, kinetic constants such as μ max = 1.15/h, K s = 35.4 mg/L and K i = 198.6 mg/L fit well phenol removal by P. pseudoalcaligenes.Although P. pseudoalcaligenes planktonic cells showed the highest phenol removal rate, in constructed wetland systems and especially in those with sub-surface flow, it is expected that surface associated microorganisms (biofilms) will provide a much higher contribution in phenol and other organics removal, due to greater bacterial biomass.Factors affecting the performance of planktonic vs. biofilm bacteria in sub-surface flow constructed wetlands are further discussed. © 2010 Elsevier Ltd.
Scientific Publication
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