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Punctured-hypocotyl method for high-efficient transformation and adventitious shoot regeneration of tomato
Year:
2014
Source of publication :
Scientia Horticulturae
Authors :
Sivankalyani, Velu
;
.
Volume :
165
Co-Authors:
Sivankalyani, V., Plant Biotechnology Division, Department of Biotechnology, Bharathiar University, Coimbatore 641046, Tamil Nadu, India
Takumi, S., Laboratory of Plant Genetics, Graduate School of Agricultural Science, Kobe University, Rokkodai-cho 1-1, Nada-ku, Kobe 657-8501, Japan
Thangasamy, S., Department of Biology, Gus R. Douglass Institute, West Virginia State University, WV 25112, United States
Ashakiran, K., DRDO-BU Centre for Life Sciences, Bharathiar University, Coimbatore 641046, Tamil Nadu, India
Girija, S., Plant Biotechnology Division, Department of Biotechnology, Bharathiar University, Coimbatore 641046, Tamil Nadu, India
Facilitators :
From page:
357
To page:
364
(
Total pages:
8
)
Abstract:
To produce transgenic tomato (Solanum lycopersicum L.), an important vegetable crop worldwide, we need an efficient transformation method to thwart multiple adverse growth conditions. In this study, for the first time we compared the efficiency of the punctured-hypocotyl and normal immersion methods of Agrobacterium-mediated transformation in Indian tomato cultivars using hypocotyl explants. The transformation factors such as bacterial density of 0.4 OD600, 40-min infection, and 48-h co-cultivation were optimal to obtain maximum transformation efficiency in the tomato cv. PKM1 among four Indian cultivars tested. The transgene integration of the tomato genome was confirmed by PCR and Southern hybridization. Transformation efficiency was greater with the punctured-hypocotyl method (55.8±0.01%) than immersion method (48.3±0.06%) and comparatively higher than the previously reported transformation efficiency in tomato. The developed method is simple, efficient and could be used to transfer agronomically important genes into the tomato genome for the potential improvement in terms of quality and quantity. © 2013 Elsevier B.V.
Note:
Related Files :
Agronomy
biotransformation
Genome
immunoassay
Punctured-hypocotyl transformation
Solanum lycopersicum
Show More
Related Content
More details
DOI :
10.1016/j.scienta.2013.11.034
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19881
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:32
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Scientific Publication
Punctured-hypocotyl method for high-efficient transformation and adventitious shoot regeneration of tomato
165
Sivankalyani, V., Plant Biotechnology Division, Department of Biotechnology, Bharathiar University, Coimbatore 641046, Tamil Nadu, India
Takumi, S., Laboratory of Plant Genetics, Graduate School of Agricultural Science, Kobe University, Rokkodai-cho 1-1, Nada-ku, Kobe 657-8501, Japan
Thangasamy, S., Department of Biology, Gus R. Douglass Institute, West Virginia State University, WV 25112, United States
Ashakiran, K., DRDO-BU Centre for Life Sciences, Bharathiar University, Coimbatore 641046, Tamil Nadu, India
Girija, S., Plant Biotechnology Division, Department of Biotechnology, Bharathiar University, Coimbatore 641046, Tamil Nadu, India
Punctured-hypocotyl method for high-efficient transformation and adventitious shoot regeneration of tomato
To produce transgenic tomato (Solanum lycopersicum L.), an important vegetable crop worldwide, we need an efficient transformation method to thwart multiple adverse growth conditions. In this study, for the first time we compared the efficiency of the punctured-hypocotyl and normal immersion methods of Agrobacterium-mediated transformation in Indian tomato cultivars using hypocotyl explants. The transformation factors such as bacterial density of 0.4 OD600, 40-min infection, and 48-h co-cultivation were optimal to obtain maximum transformation efficiency in the tomato cv. PKM1 among four Indian cultivars tested. The transgene integration of the tomato genome was confirmed by PCR and Southern hybridization. Transformation efficiency was greater with the punctured-hypocotyl method (55.8±0.01%) than immersion method (48.3±0.06%) and comparatively higher than the previously reported transformation efficiency in tomato. The developed method is simple, efficient and could be used to transfer agronomically important genes into the tomato genome for the potential improvement in terms of quality and quantity. © 2013 Elsevier B.V.
Scientific Publication
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