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Epidemiology of an aphid nontransmissible potyvirus in fields of nontransgenic and coat protein transgenic squash
Year:
1999
Source of publication :
Transgenic Research
Authors :
Gal-On, Amit
;
.
Raccah, Benjamin
;
.
Volume :
8
Co-Authors:
Fuchs, M., Department of Plant Pathology, Cornell University, New York State Agric. Exp. Station, Geneva, NY 14456, United States, INRA, Unite de Recherche Vigne et Vin, Laboratoire de Virologie, 28 rue de Herrlisheim, 68021 Colmar, France
Gal-On, A., Department of Virology, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Raccah, B., Department of Virology, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Gonsalves, D., Department of Virology, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Facilitators :
From page:
429
To page:
439
(
Total pages:
11
)
Abstract:
Spread of the aphid nontransmissible Zucchini yellow mosaic virus (ZYMV) strain MV was monitored over two consecutive years in field plots of nontransgenic and transgenic squash expressing the coat protein (CP) gene of the aphid transmissible strain FL of Watermelon mosaic virus (WMV). The experimental approach was to mechanically inoculate plants with ZYMV strain MV and to assess subsequent transmissions, assumed to be vectored by aphids, of this strain to nonmechanically inoculated plants. Strain MV was distinguished from other ZYMV isolates by a threonine at position 10 of the CP or by a distinct electrophoretic pattern of a Nla IV-digested genomic cDNA fragment generated by RT-PCR. ZYMV strain MV was not detected in fields of nontransgenic plants, but was apparently aphid transmitted to 77 of 3,700 plants (2%) in transgenic fields. Despite the availability of numerous test plants and conditions of high disease pressure but low selection pressure, an epidemic of ZYMV strain MV did not develop in fields of transgenic plants. In contrast, the aphid transmissible ZYMV strain NY was aphid-transmitted to 99% (446/450) of transgenic plants under similar conditions. The relevance of these results in assessing environmental risks of transgenic plants expressing CP transgenes is discussed.
Note:
Related Files :
gene expression
Inoculation
Pathogen-derived resistance
Risk assessment
transgenic plant
virus transmission
Zucchini yellow mosaic virus
Show More
Related Content
More details
DOI :
10.1023/A:1008935426211
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19891
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:32
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Scientific Publication
Epidemiology of an aphid nontransmissible potyvirus in fields of nontransgenic and coat protein transgenic squash
8
Fuchs, M., Department of Plant Pathology, Cornell University, New York State Agric. Exp. Station, Geneva, NY 14456, United States, INRA, Unite de Recherche Vigne et Vin, Laboratoire de Virologie, 28 rue de Herrlisheim, 68021 Colmar, France
Gal-On, A., Department of Virology, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Raccah, B., Department of Virology, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Gonsalves, D., Department of Virology, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Epidemiology of an aphid nontransmissible potyvirus in fields of nontransgenic and coat protein transgenic squash
Spread of the aphid nontransmissible Zucchini yellow mosaic virus (ZYMV) strain MV was monitored over two consecutive years in field plots of nontransgenic and transgenic squash expressing the coat protein (CP) gene of the aphid transmissible strain FL of Watermelon mosaic virus (WMV). The experimental approach was to mechanically inoculate plants with ZYMV strain MV and to assess subsequent transmissions, assumed to be vectored by aphids, of this strain to nonmechanically inoculated plants. Strain MV was distinguished from other ZYMV isolates by a threonine at position 10 of the CP or by a distinct electrophoretic pattern of a Nla IV-digested genomic cDNA fragment generated by RT-PCR. ZYMV strain MV was not detected in fields of nontransgenic plants, but was apparently aphid transmitted to 77 of 3,700 plants (2%) in transgenic fields. Despite the availability of numerous test plants and conditions of high disease pressure but low selection pressure, an epidemic of ZYMV strain MV did not develop in fields of transgenic plants. In contrast, the aphid transmissible ZYMV strain NY was aphid-transmitted to 99% (446/450) of transgenic plants under similar conditions. The relevance of these results in assessing environmental risks of transgenic plants expressing CP transgenes is discussed.
Scientific Publication
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