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The molecular and biochemical basis for varietal variation in sesquiterpene content in melon (Cucumis melo L.) rinds
Year:
2008
Source of publication :
Plant Molecular Biology
Authors :
Bar, Einat
;
.
Benyamini, Yael
;
.
Burger, Joseph
;
.
Harel-Beja, Rotem
;
.
Katzir, Nurit
;
.
Lewinsohn, Efraim
;
.
Portnoy, Vitaly H.
;
.
Schaffer, Arthur
;
.
Tadmor, Yaakov
;
.
Volume :
66
Co-Authors:
Portnoy, V., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Benyamini, Y., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Bar, E., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Harel-Beja, R., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Gepstein, S., Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel
Giovannoni, J.J., United States Department of Agriculture, Boyce Thompson Institute for Plant Research, Cornell University, Tower Road, Ithaca, NY 14853, United States
Schaffer, A.A., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, P.O. Box 6, Bet Dagan 76100, Israel
Burger, J., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Tadmor, Y., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Lewinsohn, E., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Katzir, N., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Facilitators :
From page:
647
To page:
661
(
Total pages:
15
)
Abstract:
A combined chemical, biochemical and molecular study was conducted to understand the differential accumulation of volatile sesquiterpenes in melon fruits. Sesquiterpenes were present mainly in the rinds of climacteric varieties, and a great diversity in their composition was found among varieties. Sesquiterpenes were generally absent in non-climacteric varieties. Two climacteric melon varieties, the green-fleshed 'Noy Yizre'el', and the orange-fleshed 'Dulce' were further examined. In 'Noy Yizre'el' the main sesquiterpenes accumulated are δ-cadinene, γ-cadinene and α-copaene, while α-farnesene is the main sesquiterpene in 'Dulce'. Sesquiterpene synthase activities, mainly restricted to rinds of mature fruits, were shown to generate different sesquiterpenes in each variety according to the compositions found in rinds. EST melon database mining yielded two novel cDNAs coding for members of the Tps gene family termed CmTpsNY and CmTpsDul respectively, that are 43.2% similar. Heterologous expression in E. coli of CmTpsNY produced mainly δ-copaene, α-copaene, β-caryophyllene, germacrene D, α-muurolene, γ-cadinene, δ-cadinene, and α-cadinene, while CmTpsDul produced α-farnesene only. CmTpsNY was mostly expressed in 'Noy Yizre'el' rind while CmTpsDul expression was specific to 'Dulce' rind. None of these genes was expressed in rinds of the non-climacteric 'Tam Dew' cultivar. Our results indicate that different sesquiterpene synthases encoded by different members of the Tps gene family are active in melon varieties and this specificity modulates the accumulation of sesquiterpenes. The genes are differentially transcriptionally regulated during fruit development and according to variety and are likely to be associated with chemical differences responsible for the unique aromas of melon varieties. © 2008 Springer Science+Business Media B.V.
Note:
Related Files :
Cucumis melo
Cucumis melo L.
gamma-cadinene
Genetics
metabolism
molecular genetics
Molecular structure
Show More
Related Content
More details
DOI :
10.1007/s11103-008-9296-6
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19920
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:32
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Scientific Publication
The molecular and biochemical basis for varietal variation in sesquiterpene content in melon (Cucumis melo L.) rinds
66
Portnoy, V., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Benyamini, Y., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Bar, E., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Harel-Beja, R., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Gepstein, S., Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel
Giovannoni, J.J., United States Department of Agriculture, Boyce Thompson Institute for Plant Research, Cornell University, Tower Road, Ithaca, NY 14853, United States
Schaffer, A.A., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, P.O. Box 6, Bet Dagan 76100, Israel
Burger, J., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Tadmor, Y., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Lewinsohn, E., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Katzir, N., Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
The molecular and biochemical basis for varietal variation in sesquiterpene content in melon (Cucumis melo L.) rinds
A combined chemical, biochemical and molecular study was conducted to understand the differential accumulation of volatile sesquiterpenes in melon fruits. Sesquiterpenes were present mainly in the rinds of climacteric varieties, and a great diversity in their composition was found among varieties. Sesquiterpenes were generally absent in non-climacteric varieties. Two climacteric melon varieties, the green-fleshed 'Noy Yizre'el', and the orange-fleshed 'Dulce' were further examined. In 'Noy Yizre'el' the main sesquiterpenes accumulated are δ-cadinene, γ-cadinene and α-copaene, while α-farnesene is the main sesquiterpene in 'Dulce'. Sesquiterpene synthase activities, mainly restricted to rinds of mature fruits, were shown to generate different sesquiterpenes in each variety according to the compositions found in rinds. EST melon database mining yielded two novel cDNAs coding for members of the Tps gene family termed CmTpsNY and CmTpsDul respectively, that are 43.2% similar. Heterologous expression in E. coli of CmTpsNY produced mainly δ-copaene, α-copaene, β-caryophyllene, germacrene D, α-muurolene, γ-cadinene, δ-cadinene, and α-cadinene, while CmTpsDul produced α-farnesene only. CmTpsNY was mostly expressed in 'Noy Yizre'el' rind while CmTpsDul expression was specific to 'Dulce' rind. None of these genes was expressed in rinds of the non-climacteric 'Tam Dew' cultivar. Our results indicate that different sesquiterpene synthases encoded by different members of the Tps gene family are active in melon varieties and this specificity modulates the accumulation of sesquiterpenes. The genes are differentially transcriptionally regulated during fruit development and according to variety and are likely to be associated with chemical differences responsible for the unique aromas of melon varieties. © 2008 Springer Science+Business Media B.V.
Scientific Publication
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