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Development of a transgenic early flowering pear (Pyrus communis L.) genotype by RNAi silencing of PcTFL1-1 and PcTFL1-2
Year:
2012
Source of publication :
Planta
Authors :
Cohen, Yuval
;
.
Flaishman, Moshe
;
.
Golobovich, Sara
;
.
Korchinsky, Raia
;
.
Shlizerman, Lyudmila A.
;
.
Yablowitz, Zeev
;
.
Volume :
235
Co-Authors:
Freiman, A., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel, The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Shlizerman, L., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel
Golobovitch, S., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel
Yablovitz, Z., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel
Korchinsky, R., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel
Cohen, Y., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel
Samach, A., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Chevreau, E., INRA,Institut de Recherche en Horticulture et Semences(INRA, Agrocampus-Ouest, Universite d'Angers), SFR 149 QUASAV, 49071 Beaucouzé, France
Le Roux, P.-M., Agroscope Changins-Wädenswil Research Station (ACW), Schloss 1, 8820 Wädenswil, Switzerland
Patocchi, A., Agroscope Changins-Wädenswil Research Station (ACW), Schloss 1, 8820 Wädenswil, Switzerland
Flaishman, M.A., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel
Facilitators :
From page:
1239
To page:
1251
(
Total pages:
13
)
Abstract:
Trees require a long maturation period, known as juvenile phase, before they can reproduce, complicating their genetic improvement as compared to annual plants. 'Spadona', one of the most important European pear (Pyrus communis L.) cultivars grown in Israel, has a very long juvenile period, up to 14 years, making breeding programs extremely slow. Progress in understanding the molecular basis of the transition to flowering has revealed genes that accelerate reproductive development when ectopically expressed in transgenic plants. A transgenic line of 'Spadona', named Early Flowering-Spadona (EF-Spa), was produced using a MdTFL1 RNAi cassette targeting the native pear genes PcTFL1-1 and PcTFL1-2. The transgenic line had three T-DNA insertions, one assigned to chromosome 2 and two to chromosome 14 PcTFL1-1 and PcTFL1-2 were completely silenced, and EF-Spa displayed an early flowering phenotype: flowers developed already in tissue culture and on most rooted plants 1-8 months after transfer to the greenhouse. EF-Spa developed solitary flowers from apical or lateral buds, reducing vegetative growth vigor. Pollination of EF-Spa trees generated normal-shaped fruits with viable F1 seeds. The greenhouse-grown transgenic F1 seedlings formed shoots and produced flowers 1-33 months after germination. Sequence analyses, of the non-transgenic F1 seedlings, demonstrated that this approach can be used to recover seedlings that have no trace of the T-DNA. Thus, the early flowering transgenic line EF-Spa obtained by PcTFL1 silencing provides an interesting tool to accelerate pear breeding. © 2011 Springer-Verlag.
Note:
Related Files :
Genetics
genetic transformation
juvenility
molecular genetics
phenotype
Pyrus communis
temperature
Show More
Related Content
More details
DOI :
10.1007/s00425-011-1571-0
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19954
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:32
You may also be interested in
Scientific Publication
Development of a transgenic early flowering pear (Pyrus communis L.) genotype by RNAi silencing of PcTFL1-1 and PcTFL1-2
235
Freiman, A., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel, The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Shlizerman, L., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel
Golobovitch, S., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel
Yablovitz, Z., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel
Korchinsky, R., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel
Cohen, Y., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel
Samach, A., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Chevreau, E., INRA,Institut de Recherche en Horticulture et Semences(INRA, Agrocampus-Ouest, Universite d'Angers), SFR 149 QUASAV, 49071 Beaucouzé, France
Le Roux, P.-M., Agroscope Changins-Wädenswil Research Station (ACW), Schloss 1, 8820 Wädenswil, Switzerland
Patocchi, A., Agroscope Changins-Wädenswil Research Station (ACW), Schloss 1, 8820 Wädenswil, Switzerland
Flaishman, M.A., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel
Development of a transgenic early flowering pear (Pyrus communis L.) genotype by RNAi silencing of PcTFL1-1 and PcTFL1-2
Trees require a long maturation period, known as juvenile phase, before they can reproduce, complicating their genetic improvement as compared to annual plants. 'Spadona', one of the most important European pear (Pyrus communis L.) cultivars grown in Israel, has a very long juvenile period, up to 14 years, making breeding programs extremely slow. Progress in understanding the molecular basis of the transition to flowering has revealed genes that accelerate reproductive development when ectopically expressed in transgenic plants. A transgenic line of 'Spadona', named Early Flowering-Spadona (EF-Spa), was produced using a MdTFL1 RNAi cassette targeting the native pear genes PcTFL1-1 and PcTFL1-2. The transgenic line had three T-DNA insertions, one assigned to chromosome 2 and two to chromosome 14 PcTFL1-1 and PcTFL1-2 were completely silenced, and EF-Spa displayed an early flowering phenotype: flowers developed already in tissue culture and on most rooted plants 1-8 months after transfer to the greenhouse. EF-Spa developed solitary flowers from apical or lateral buds, reducing vegetative growth vigor. Pollination of EF-Spa trees generated normal-shaped fruits with viable F1 seeds. The greenhouse-grown transgenic F1 seedlings formed shoots and produced flowers 1-33 months after germination. Sequence analyses, of the non-transgenic F1 seedlings, demonstrated that this approach can be used to recover seedlings that have no trace of the T-DNA. Thus, the early flowering transgenic line EF-Spa obtained by PcTFL1 silencing provides an interesting tool to accelerate pear breeding. © 2011 Springer-Verlag.
Scientific Publication
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