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Magic-angle spinning NMR studies of cell wall bound aromatic-aliphatic biopolyesters associated with strengthening of intercellular adhesion in potato (Solanum tuberosum L.) tuber parenchyma
Year:
2006
Source of publication :
Biomacromolecules
Authors :
Gaba, Victor
;
.
Jashi, Liana
;
.
Shomer, Ilan
;
.
Vengadesan, G.
;
.
Yefremov, Tatiana
;
.
Volume :
7
Co-Authors:
Yu, B., College of Staten Island, Department of Chemistry, City University of New York, 2800 Victory Boulevard, Staten Island, NY 10314-6600, United States
Vengadesan, G., Department of Food Science, Agriculture Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Wang, H., College of Staten Island, Department of Chemistry, City University of New York, 2800 Victory Boulevard, Staten Island, NY 10314-6600, United States
Jashi, L., Department of Food Science, Agriculture Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Yefremov, T., Department of Food Science, Agriculture Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Tian, S., College of Staten Island, Department of Chemistry, City University of New York, 2800 Victory Boulevard, Staten Island, NY 10314-6600, United States
Gaba, V., Department of Food Science, Agriculture Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Shomer, I., Department of Food Science, Agriculture Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Stark, R.E., College of Staten Island, Department of Chemistry, City University of New York, 2800 Victory Boulevard, Staten Island, NY 10314-6600, United States
Facilitators :
From page:
937
To page:
944
(
Total pages:
8
)
Abstract:
Intercellular adhesion strengthening, a phenomenon that compromises the texture and the edible quality of potatoes (Solanum tuberosum L.), has been induced reproducibly by exposure to low-pH acetic acid solutions under tissue culture conditions. The resulting parenchyma tissues have been examined by solid-state nuclear magnetic resonance (NMR) in order to characterize the biopolymer(s) thought to be associated with this syndrome. Cross polarization-magic angle spinning (CPMAS) 13C NMR has been used to establish the presence of a polyphenol-suberin-like aromatic-aliphatic polyester within an abundant cell wall polysaccharide matrix in potato tubers that exhibit hardening due to strengthened intercellular adhesion. Dipolar dephasing and CP chemical shift anisotropy experiments suggest that the aromatic domain is composed primarily of guaiacyl and sinapyl groups. Two-dimensional wide-line separation experiments show that the biopolymer associated with parenchyma hardening contains rigid polysaccharide cell walls and mobile aliphatic long-chain fatty acids; 1H spin diffusion experiments show that these flexible aliphatic chains are proximal to both the phenolics and a subpopulation of the cell wall polysaccharides. Finally, high-resolution MAS NMR of parenchyma samples swelled in DMSO in conjunction with two-dimensional through-bond and through-space NMR spectroscopy provides evidence for covalent linkages among the polysaccharide, phenolic, and aliphatic domains of the intercellular adhesion-strengthening biopolymer in potato parenchyma tissue. © 2006 American Chemical Society.
Note:
Related Files :
Carbon
cellular distribution
covalent bond
flavonoids
food quality
phenols
Plant Cell
Solanum tuberosum
Show More
Related Content
More details
DOI :
10.1021/bm050812t
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19962
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:33
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Scientific Publication
Magic-angle spinning NMR studies of cell wall bound aromatic-aliphatic biopolyesters associated with strengthening of intercellular adhesion in potato (Solanum tuberosum L.) tuber parenchyma
7
Yu, B., College of Staten Island, Department of Chemistry, City University of New York, 2800 Victory Boulevard, Staten Island, NY 10314-6600, United States
Vengadesan, G., Department of Food Science, Agriculture Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Wang, H., College of Staten Island, Department of Chemistry, City University of New York, 2800 Victory Boulevard, Staten Island, NY 10314-6600, United States
Jashi, L., Department of Food Science, Agriculture Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Yefremov, T., Department of Food Science, Agriculture Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Tian, S., College of Staten Island, Department of Chemistry, City University of New York, 2800 Victory Boulevard, Staten Island, NY 10314-6600, United States
Gaba, V., Department of Food Science, Agriculture Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Shomer, I., Department of Food Science, Agriculture Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Stark, R.E., College of Staten Island, Department of Chemistry, City University of New York, 2800 Victory Boulevard, Staten Island, NY 10314-6600, United States
Magic-angle spinning NMR studies of cell wall bound aromatic-aliphatic biopolyesters associated with strengthening of intercellular adhesion in potato (Solanum tuberosum L.) tuber parenchyma
Intercellular adhesion strengthening, a phenomenon that compromises the texture and the edible quality of potatoes (Solanum tuberosum L.), has been induced reproducibly by exposure to low-pH acetic acid solutions under tissue culture conditions. The resulting parenchyma tissues have been examined by solid-state nuclear magnetic resonance (NMR) in order to characterize the biopolymer(s) thought to be associated with this syndrome. Cross polarization-magic angle spinning (CPMAS) 13C NMR has been used to establish the presence of a polyphenol-suberin-like aromatic-aliphatic polyester within an abundant cell wall polysaccharide matrix in potato tubers that exhibit hardening due to strengthened intercellular adhesion. Dipolar dephasing and CP chemical shift anisotropy experiments suggest that the aromatic domain is composed primarily of guaiacyl and sinapyl groups. Two-dimensional wide-line separation experiments show that the biopolymer associated with parenchyma hardening contains rigid polysaccharide cell walls and mobile aliphatic long-chain fatty acids; 1H spin diffusion experiments show that these flexible aliphatic chains are proximal to both the phenolics and a subpopulation of the cell wall polysaccharides. Finally, high-resolution MAS NMR of parenchyma samples swelled in DMSO in conjunction with two-dimensional through-bond and through-space NMR spectroscopy provides evidence for covalent linkages among the polysaccharide, phenolic, and aliphatic domains of the intercellular adhesion-strengthening biopolymer in potato parenchyma tissue. © 2006 American Chemical Society.
Scientific Publication
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