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Physiological and biochemical changes at the rootstock-scion interface in graft combinations between Cucurbita rootstocks and a melon scion
Year:
2008
Authors :
Aloni, Benyamin
;
.
Cohen, Roni
;
.
Deventurero, Gila
;
.
Edelstein, Menahem
;
.
Horev, Carmela
;
.
Joel, Daniel M.
;
.
Kapulnik, Yoram
;
.
Karni, Leah
;
.
Katzir, Nurit
;
.
Levin, Ziv
;
.
Volume :
83
Co-Authors:
Aloni, B., Department of Vegetable Research, Institute of Plant Science, A.R.O. the Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Karni, L., Department of Vegetable Research, Institute of Plant Science, A.R.O. the Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Deventurero, G., Department of Vegetable Research, Institute of Plant Science, A.R.O. the Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Levin, Z., Department of Vegetable Research, Institute of Plant Science, A.R.O. the Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Cohen, R., Department of Vegetable Research, A.R.O. the Volcani Center, Institute of Plant Science, P.O. Box 1021, Ramat-Yishay 30095, Israel
Katzir, N., Department of Vegetable Research, A.R.O. the Volcani Center, Institute of Plant Science, P.O. Box 1021, Ramat-Yishay 30095, Israel
Lotan-Pompan, M., Department of Vegetable Research, A.R.O. the Volcani Center, Institute of Plant Science, P.O. Box 1021, Ramat-Yishay 30095, Israel
Edelstein, M., Department of Vegetable Research, A.R.O. the Volcani Center, Institute of Plant Science, P.O. Box 1021, Ramat-Yishay 30095, Israel
Aktas, H., Department of Horticulture, Faculty of Agriculture, Harran University, 63040 Sanlmrfa, Turkey
Turhan, E., Department of Horticulture, Faculty of Agriculture, Eskisehir Osmangazi University, 26160 Eskisehir, Turkey
Joel, D.M., Department of Plant Pathology and Weed Research, A.R.O. Northern R and D Center, Newe-Ya'ar 30095, Israel
Horev, C., Department of Plant Pathology and Weed Research, A.R.O. Northern R and D Center, Newe-Ya'ar 30095, Israel
Kapulnik, Y., Department of Agronomy and Natural Resources, Institute of Plant Science, A.R.O. the Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Facilitators :
From page:
777
To page:
783
(
Total pages:
7
)
Abstract:
Success in grafting depends on the identification of a compatible rootstock (RS) that promotes rapid formation of vascular connections between the RS and the scion, and rapid resumption of root and shoot growth. In this study, we used compatible and incompatible Cucurbita rootstocks, with a melon (Cucumis melo L. 'Arava') scion, to identify physiological and biochemical factors in the scion-RS interface that could be associated with graft compatibility. Anatomical characterisation of the grafting interface showed that the regeneration and differentiation of vascular elements was similar, at first, in both compatible and incompatible grafted seedlings although, in the latter, a protective layer was formed, but did not interfere with communication between the RS and the scion. Water uptake and sugar distribution between the plant canopy and the roots, measured 14 d after grafting (DAG) were not correlated with compatibility or incompatibility. At 24 DAG, both water uptake and root sugar concentrations decreased significantly in the incompatible RS, reflecting deterioration of the RS. At 24 DAG, part of the RS collapsed, but only in incompatible grafted seedlings. Histochemical staining revealed that superoxide, H20 2, peroxidase (POX) activity, and lignin deposits at the scion-rootstock interface were similar in both compatible and incompatible grafting combinations at 14 DAG; but, at 24 DAG, H2O2 and superoxide levels were higher in the incompatible grafted transplants. In addition, cell-wall POX and superoxide dismutase (SOD) activities were lower in the incompatible RS-scion interface at 14 and 24 DAG. These results suggest that a physical barrier is unlikely to be formed between the incompatible partners early after grafting, but that lower anti-oxidant enzyme activities and higher levels of reactive oxygen species (ROS) in the incompatible RS-scion interface may be responsible for degradation of the grafting zone.
Note:
Related Files :
Cucumis
Cucumis melo
Cucurbita
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DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19967
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:33
Scientific Publication
Physiological and biochemical changes at the rootstock-scion interface in graft combinations between Cucurbita rootstocks and a melon scion
83
Aloni, B., Department of Vegetable Research, Institute of Plant Science, A.R.O. the Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Karni, L., Department of Vegetable Research, Institute of Plant Science, A.R.O. the Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Deventurero, G., Department of Vegetable Research, Institute of Plant Science, A.R.O. the Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Levin, Z., Department of Vegetable Research, Institute of Plant Science, A.R.O. the Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Cohen, R., Department of Vegetable Research, A.R.O. the Volcani Center, Institute of Plant Science, P.O. Box 1021, Ramat-Yishay 30095, Israel
Katzir, N., Department of Vegetable Research, A.R.O. the Volcani Center, Institute of Plant Science, P.O. Box 1021, Ramat-Yishay 30095, Israel
Lotan-Pompan, M., Department of Vegetable Research, A.R.O. the Volcani Center, Institute of Plant Science, P.O. Box 1021, Ramat-Yishay 30095, Israel
Edelstein, M., Department of Vegetable Research, A.R.O. the Volcani Center, Institute of Plant Science, P.O. Box 1021, Ramat-Yishay 30095, Israel
Aktas, H., Department of Horticulture, Faculty of Agriculture, Harran University, 63040 Sanlmrfa, Turkey
Turhan, E., Department of Horticulture, Faculty of Agriculture, Eskisehir Osmangazi University, 26160 Eskisehir, Turkey
Joel, D.M., Department of Plant Pathology and Weed Research, A.R.O. Northern R and D Center, Newe-Ya'ar 30095, Israel
Horev, C., Department of Plant Pathology and Weed Research, A.R.O. Northern R and D Center, Newe-Ya'ar 30095, Israel
Kapulnik, Y., Department of Agronomy and Natural Resources, Institute of Plant Science, A.R.O. the Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Physiological and biochemical changes at the rootstock-scion interface in graft combinations between Cucurbita rootstocks and a melon scion
Success in grafting depends on the identification of a compatible rootstock (RS) that promotes rapid formation of vascular connections between the RS and the scion, and rapid resumption of root and shoot growth. In this study, we used compatible and incompatible Cucurbita rootstocks, with a melon (Cucumis melo L. 'Arava') scion, to identify physiological and biochemical factors in the scion-RS interface that could be associated with graft compatibility. Anatomical characterisation of the grafting interface showed that the regeneration and differentiation of vascular elements was similar, at first, in both compatible and incompatible grafted seedlings although, in the latter, a protective layer was formed, but did not interfere with communication between the RS and the scion. Water uptake and sugar distribution between the plant canopy and the roots, measured 14 d after grafting (DAG) were not correlated with compatibility or incompatibility. At 24 DAG, both water uptake and root sugar concentrations decreased significantly in the incompatible RS, reflecting deterioration of the RS. At 24 DAG, part of the RS collapsed, but only in incompatible grafted seedlings. Histochemical staining revealed that superoxide, H20 2, peroxidase (POX) activity, and lignin deposits at the scion-rootstock interface were similar in both compatible and incompatible grafting combinations at 14 DAG; but, at 24 DAG, H2O2 and superoxide levels were higher in the incompatible grafted transplants. In addition, cell-wall POX and superoxide dismutase (SOD) activities were lower in the incompatible RS-scion interface at 14 and 24 DAG. These results suggest that a physical barrier is unlikely to be formed between the incompatible partners early after grafting, but that lower anti-oxidant enzyme activities and higher levels of reactive oxygen species (ROS) in the incompatible RS-scion interface may be responsible for degradation of the grafting zone.
Scientific Publication
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