נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Nitrate and phosphate regimes induced lipidomic and biochemical changes in the intertidal macroalga ulva lactuca (ulvophyceae, chlorophyta)
Year:
2014
Source of publication :
Plant and Cell Physiology
Authors :
Kumar, Manoj
;
.
Kumari, Puja
;
.
Volume :
55
Co-Authors:
Kumari, P., Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt, Marine Chemicals Research Institute, Bhavnagar 364002, Gujarat, India
Kumar, M., Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt, Marine Chemicals Research Institute, Bhavnagar 364002, Gujarat, India, Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Reddy, C.R.K., Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt, Marine Chemicals Research Institute, Bhavnagar 364002, Gujarat, India
Jha, B., Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt, Marine Chemicals Research Institute, Bhavnagar 364002, Gujarat, India
Facilitators :
From page:
52
To page:
63
(
Total pages:
12
)
Abstract:
This study was carried out in order to understand the lipid and biochemical alterations resulting from different nutritional regimes of nitrate and phosphate in Ulva lactuca. The algal thalli cultured in artificial seawater (ASW) showed higher levels of carbohydrates and non-polar lipids and increased phosphatase activities, accompanied by degradation of polar lipids, proteins and pigments. Further, higher levels of lipid hydroperoxides indicated reative oxygen species (ROS)-mediated non-enzymatic lipid peroxidation due to nutritional limitation-induced oxidative stress. Those thalli cultured in ASW supplemented with nitrate showed responses corresponding to nitrate addition, such as an increase in pigments, monogalactosyldiacylglycerols, polyunsaturated fatty acids and nitrate reductase. In addition, these thalli showed partial induction of phosphatases, low phospholipids, and high sulfolipid and 1,2-diacylglyceryl-3-O-4′-(N,N,N-trimethyl)-homoserine (DGTS) due to phosphate limitation. Similarly, algal thalli cultured in ASW supplemented with phosphate showed down-regulation of phosphatases, an increase in phospholipids due to availability of phosphate as well as a decrease in nitrate reductase, pigment, monogalactosyldiacylglycerols and polyunsaturated fatty acids due to nitrate limitation. On the other hand, algal thalli cultured in ASW supplemented with both nitrate and phosphate showed recovery of lost pigments and proteins, a high monogalactosyldiacylglycerol/digalactosyldiacylglycerol ratio, high unsaturation and high oxylipin levels (both C18 and C20). Further, the accumulation of indole-3-acetic acid in nutrient-limited thalli and of kinetin and kinetin riboside in nutrient-supplemented thalli indicated their antagonistic roles under nutrient stress. Thus, U. lactuca copes with nitrate and phosphate nutritional stress by altering the metabolic pathways involved in lipid biosynthesis including a shift in lipid classes, fatty acids, oxylipins and indole-3-acetic acid/kinetin cross-talk. © 2013 The Author.
Note:
Related Files :
drug effect
fatty acids
Growth, Development and Aging
Lipids
metabolism
Nitrates
Ulva lactuca
water flow
Show More
Related Content
More details
DOI :
10.1093/pcp/pct156
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19976
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:33
You may also be interested in
Scientific Publication
Nitrate and phosphate regimes induced lipidomic and biochemical changes in the intertidal macroalga ulva lactuca (ulvophyceae, chlorophyta)
55
Kumari, P., Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt, Marine Chemicals Research Institute, Bhavnagar 364002, Gujarat, India
Kumar, M., Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt, Marine Chemicals Research Institute, Bhavnagar 364002, Gujarat, India, Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Reddy, C.R.K., Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt, Marine Chemicals Research Institute, Bhavnagar 364002, Gujarat, India
Jha, B., Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt, Marine Chemicals Research Institute, Bhavnagar 364002, Gujarat, India
Nitrate and phosphate regimes induced lipidomic and biochemical changes in the intertidal macroalga ulva lactuca (ulvophyceae, chlorophyta)
This study was carried out in order to understand the lipid and biochemical alterations resulting from different nutritional regimes of nitrate and phosphate in Ulva lactuca. The algal thalli cultured in artificial seawater (ASW) showed higher levels of carbohydrates and non-polar lipids and increased phosphatase activities, accompanied by degradation of polar lipids, proteins and pigments. Further, higher levels of lipid hydroperoxides indicated reative oxygen species (ROS)-mediated non-enzymatic lipid peroxidation due to nutritional limitation-induced oxidative stress. Those thalli cultured in ASW supplemented with nitrate showed responses corresponding to nitrate addition, such as an increase in pigments, monogalactosyldiacylglycerols, polyunsaturated fatty acids and nitrate reductase. In addition, these thalli showed partial induction of phosphatases, low phospholipids, and high sulfolipid and 1,2-diacylglyceryl-3-O-4′-(N,N,N-trimethyl)-homoserine (DGTS) due to phosphate limitation. Similarly, algal thalli cultured in ASW supplemented with phosphate showed down-regulation of phosphatases, an increase in phospholipids due to availability of phosphate as well as a decrease in nitrate reductase, pigment, monogalactosyldiacylglycerols and polyunsaturated fatty acids due to nitrate limitation. On the other hand, algal thalli cultured in ASW supplemented with both nitrate and phosphate showed recovery of lost pigments and proteins, a high monogalactosyldiacylglycerol/digalactosyldiacylglycerol ratio, high unsaturation and high oxylipin levels (both C18 and C20). Further, the accumulation of indole-3-acetic acid in nutrient-limited thalli and of kinetin and kinetin riboside in nutrient-supplemented thalli indicated their antagonistic roles under nutrient stress. Thus, U. lactuca copes with nitrate and phosphate nutritional stress by altering the metabolic pathways involved in lipid biosynthesis including a shift in lipid classes, fatty acids, oxylipins and indole-3-acetic acid/kinetin cross-talk. © 2013 The Author.
Scientific Publication
You may also be interested in