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Growth characteristics and stability of tolerance of citrus callus cells subjected to NaCl stress
Year:
1982
Source of publication :
Plant Science Letters
Authors :
Ben Hayyim, Gosal
;
.
Kochba, Joshuah
;
.
Volume :
27
Co-Authors:
Ben-Hayyim, G., Institute of Horticulture, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet-Dagan, Israel
Kochba, J., Institute of Horticulture, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet-Dagan, Israel
Facilitators :
From page:
87
To page:
94
(
Total pages:
8
)
Abstract:
Callus cells of 'Shamouti' (Citrus sinensis Osb.) capable of growing in the presence of 0.2 M NaCl were obtained by exposure of the cultured cells to a medium containing salt. The growth curve of the selected salt-tolerant line (R-10) in the presence of NaCl showed a prolonged lag period, slightly slower rate of increase in fresh weight and about 50% yield as compared with the situation in the absence of salt. However, non-selected cells (L-5) did not grow in the presence of 0.2 M NaCl. The increased tolerance for salt of the R-10 cell line was retained after four consecutive transfers in medium without salt. This stability indicates that during the selection procedure a true genetic variant was isolated. R-10 cells selected for tolerance towards NaCl were also tolerant to other sodium salts. © 1982.
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More details
DOI :
10.1016/0304-4211(82)90075-X
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
19998
Last updated date:
18/12/2023 13:55
Creation date:
16/04/2018 23:33
Scientific Publication
Growth characteristics and stability of tolerance of citrus callus cells subjected to NaCl stress
27
Ben-Hayyim, G., Institute of Horticulture, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet-Dagan, Israel
Kochba, J., Institute of Horticulture, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet-Dagan, Israel
Growth characteristics and stability of tolerance of citrus callus cells subjected to NaCl stress
Callus cells of 'Shamouti' (Citrus sinensis Osb.) capable of growing in the presence of 0.2 M NaCl were obtained by exposure of the cultured cells to a medium containing salt. The growth curve of the selected salt-tolerant line (R-10) in the presence of NaCl showed a prolonged lag period, slightly slower rate of increase in fresh weight and about 50% yield as compared with the situation in the absence of salt. However, non-selected cells (L-5) did not grow in the presence of 0.2 M NaCl. The increased tolerance for salt of the R-10 cell line was retained after four consecutive transfers in medium without salt. This stability indicates that during the selection procedure a true genetic variant was isolated. R-10 cells selected for tolerance towards NaCl were also tolerant to other sodium salts. © 1982.
Scientific Publication
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