אסיף מאגר המחקר החקלאי

Growth characteristics and stability of tolerance of citrus callus cells subjected to NaCl stress

Year:

1982

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Volume :

27

Co-Authors:

Ben-Hayyim, G., Institute of Horticulture, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet-Dagan, Israel
Kochba, J., Institute of Horticulture, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet-Dagan, Israel

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Abstract:

Callus cells of 'Shamouti' (Citrus sinensis Osb.) capable of growing in the presence of 0.2 M NaCl were obtained by exposure of the cultured cells to a medium containing salt. The growth curve of the selected salt-tolerant line (R-10) in the presence of NaCl showed a prolonged lag period, slightly slower rate of increase in fresh weight and about 50% yield as compared with the situation in the absence of salt. However, non-selected cells (L-5) did not grow in the presence of 0.2 M NaCl. The increased tolerance for salt of the R-10 cell line was retained after four consecutive transfers in medium without salt. This stability indicates that during the selection procedure a true genetic variant was isolated. R-10 cells selected for tolerance towards NaCl were also tolerant to other sodium salts. © 1982.

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