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Indole-3-butyric acid in Arabidopsis thaliana - I. Identification and quantification
Year:
1993
Source of publication :
Plant Growth Regulation
Authors :
Wodner, Maria
;
.
Volume :
13
Co-Authors:
Ludwig-Müller, J., Botanisches Institut, J.W. Goethe-Universität, Siesmayerstr. 70, Frankfurt am Main, 6000, Germany
Sass, S., Botanisches Institut, J.W. Goethe-Universität, Siesmayerstr. 70, Frankfurt am Main, 6000, Germany
Sutter, E.G., Department of Pomology, University of California, Davis, 95616, CA, United States
Wodner, M., Department of Horticulture, Volcani Center, ARO, Bet Dagan, Israel
Epstein, E., Department of Horticulture, Volcani Center, ARO, Bet Dagan, Israel
Facilitators :
From page:
179
To page:
187
(
Total pages:
9
)
Abstract:
Indole-3-butyric acid (IBA) was identified by HPLC and GC-MS as an endogenous compound in plantlets of the crucifer Arabidopsis thaliana (L.) Heynh. A. thaliana was cultivated under sterile conditions as shaking culture in different liquid media with and without supply of hormones. Free and total IBA and indole-3-acetic acid (IAA) were determined at different stages of development during the culture period as well as in culture media of different initial pH values. The results showed that IAA was present in higher concentrations than IBA, but both hormones seemed to show the same behaviour under the different experimental conditions. Differences were found in the mode of conjugation of the two hormones. While IAA was mostly conjugated via amide bonds, the main IBA conjugates were ester bound. The ethylene concentration derived from the seedlings, when they were grown in flasks of different size, seemed not to influence the auxin content in the same cultures. © 1993 Kluwer Academic Publishers.
Note:
Related Files :
Arabidopsis thaliana
auxin conjugates
ethylene
Indole-3-acetic acid
indole-3-butyric acid
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Related Content
More details
DOI :
10.1007/BF00024260
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
20032
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:33
Scientific Publication
Indole-3-butyric acid in Arabidopsis thaliana - I. Identification and quantification
13
Ludwig-Müller, J., Botanisches Institut, J.W. Goethe-Universität, Siesmayerstr. 70, Frankfurt am Main, 6000, Germany
Sass, S., Botanisches Institut, J.W. Goethe-Universität, Siesmayerstr. 70, Frankfurt am Main, 6000, Germany
Sutter, E.G., Department of Pomology, University of California, Davis, 95616, CA, United States
Wodner, M., Department of Horticulture, Volcani Center, ARO, Bet Dagan, Israel
Epstein, E., Department of Horticulture, Volcani Center, ARO, Bet Dagan, Israel
Indole-3-butyric acid in Arabidopsis thaliana - I. Identification and quantification
Indole-3-butyric acid (IBA) was identified by HPLC and GC-MS as an endogenous compound in plantlets of the crucifer Arabidopsis thaliana (L.) Heynh. A. thaliana was cultivated under sterile conditions as shaking culture in different liquid media with and without supply of hormones. Free and total IBA and indole-3-acetic acid (IAA) were determined at different stages of development during the culture period as well as in culture media of different initial pH values. The results showed that IAA was present in higher concentrations than IBA, but both hormones seemed to show the same behaviour under the different experimental conditions. Differences were found in the mode of conjugation of the two hormones. While IAA was mostly conjugated via amide bonds, the main IBA conjugates were ester bound. The ethylene concentration derived from the seedlings, when they were grown in flasks of different size, seemed not to influence the auxin content in the same cultures. © 1993 Kluwer Academic Publishers.
Scientific Publication
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