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Reassessment of the in vivo Assay for Nitrate Reductase in Leaves
Year:
1978
Source of publication :
Physiologia Plantarum
Authors :
Heuer, Bruria
;
.
Plaut, Zvi
;
.
Volume :
43
Co-Authors:
HEUER, B., Agricultural Research Organization, The Volcani Center, The Institute of Soils and Water, P.O.B. 6, Bet Dagan, Israel
PLAUT, Z., Agricultural Research Organization, The Volcani Center, The Institute of Soils and Water, P.O.B. 6, Bet Dagan, Israel
Facilitators :
From page:
306
To page:
312
(
Total pages:
7
)
Abstract:
The in vivo assay procedure for nitrate reductase and its dependence on the concentration of nitrate and other ions were examined. It was found that high ion concentrations led to an increased release of nitrite to the reaction media which could be interpreted as a stimulated nitrate reductase activity. This phenomenon is not an osmotic effect, since equivalent concentrations of mannitol did not lead to identical results. The effect of ions on the enhanced nitrite production was attributed to changes in cell membrane permeability rather than to a supply of substrate. This conclusion is based on several findings: (a) in in vitro assays, the rate of nitrite production was not affected by ion concentrations: (b) the stimulation of nitrite production was obtained by various ions and not only by nitrate; (c) pretreatment of alfalfa leaves with nitrate did not increase the NO2 − release rate to the external solution; and (d) nitrate and nitrite export from leaf discs to the solution was stimulated even in discs which were enzymatically inactive. Calcium ions in the presence of KNO3 inhibited the enhanced nitrite production, probably due to alteration of membrane stability. The effect of ions on the rate of nitrite production was reversible and the high rate of nitrite production was reduced to the control rate when discs were transferred to a solution of low concentration. Copyright © 1978, Wiley Blackwell. All rights reserved
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More details
DOI :
10.1111/j.1399-3054.1978.tb02583.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
20079
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:33
Scientific Publication
Reassessment of the in vivo Assay for Nitrate Reductase in Leaves
43
HEUER, B., Agricultural Research Organization, The Volcani Center, The Institute of Soils and Water, P.O.B. 6, Bet Dagan, Israel
PLAUT, Z., Agricultural Research Organization, The Volcani Center, The Institute of Soils and Water, P.O.B. 6, Bet Dagan, Israel
Reassessment of the in vivo Assay for Nitrate Reductase in Leaves
The in vivo assay procedure for nitrate reductase and its dependence on the concentration of nitrate and other ions were examined. It was found that high ion concentrations led to an increased release of nitrite to the reaction media which could be interpreted as a stimulated nitrate reductase activity. This phenomenon is not an osmotic effect, since equivalent concentrations of mannitol did not lead to identical results. The effect of ions on the enhanced nitrite production was attributed to changes in cell membrane permeability rather than to a supply of substrate. This conclusion is based on several findings: (a) in in vitro assays, the rate of nitrite production was not affected by ion concentrations: (b) the stimulation of nitrite production was obtained by various ions and not only by nitrate; (c) pretreatment of alfalfa leaves with nitrate did not increase the NO2 − release rate to the external solution; and (d) nitrate and nitrite export from leaf discs to the solution was stimulated even in discs which were enzymatically inactive. Calcium ions in the presence of KNO3 inhibited the enhanced nitrite production, probably due to alteration of membrane stability. The effect of ions on the rate of nitrite production was reversible and the high rate of nitrite production was reduced to the control rate when discs were transferred to a solution of low concentration. Copyright © 1978, Wiley Blackwell. All rights reserved
Scientific Publication
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