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Isolation of human erythrocyte membranes and their outer surface proteins by covalent fractionation on solid support
Year:
1979
Source of publication :
FEBS Letters
Authors :
Eshdat, Yuval
;
.
Volume :
101
Co-Authors:
Eshdat, Y., Dept. Biophys., Weizmann Inst. Sci., Rehovot, Israel
Prujansky-Jakobovits, A., Dept. Biophys., Weizmann Inst. Sci., Rehovot, Israel
Facilitators :
From page:
43
To page:
46
(
Total pages:
4
)
Abstract:
We describe a novel method for the isolation of human erythrocyte membranes based on the covalent binding of the cells to a solid support via cleavable disulfide bonds. After attachment, the cells are lysed, washed and the resultant membranes detached from the support by mild reduction with dithiothreitol. The application of this method to the isolation and identification of outer cell surface proteins is described.
Note:
Related Files :
erythrocyte membrane
human
human cell
membrane protein
Membrane Proteins
methodology
Sepharose
Show More
Related Content
More details
DOI :
10.1016/0014-5793(79)81291-0
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
20082
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:33
Scientific Publication
Isolation of human erythrocyte membranes and their outer surface proteins by covalent fractionation on solid support
101
Eshdat, Y., Dept. Biophys., Weizmann Inst. Sci., Rehovot, Israel
Prujansky-Jakobovits, A., Dept. Biophys., Weizmann Inst. Sci., Rehovot, Israel
Isolation of human erythrocyte membranes and their outer surface proteins by covalent fractionation on solid support
We describe a novel method for the isolation of human erythrocyte membranes based on the covalent binding of the cells to a solid support via cleavable disulfide bonds. After attachment, the cells are lysed, washed and the resultant membranes detached from the support by mild reduction with dithiothreitol. The application of this method to the isolation and identification of outer cell surface proteins is described.
Scientific Publication
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