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Differential expression of miRNAs and their target genes in senescing leaves and siliques: Insights from deep sequencing of small RNAs and cleaved target RNAs
Year:
2015
Source of publication :
Plant, Cell and Environment
Authors :
Burd, Shaul
;
.
Lers, Amnon
;
.
Volume :
38
Co-Authors:
Thatcher, S.R., Delaware Biotechnology Institute, University of Delaware, Newark, DE, United States, DuPont Pioneer, Wilmington, DE, United States
Burd, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Wright, C., Delaware Biotechnology Institute, University of Delaware, Newark, DE, United States, Princeton University, Princeton, NJ, United States
Lers, A., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Green, P.J., Delaware Biotechnology Institute, University of Delaware, Newark, DE, United States
Facilitators :
From page:
188
To page:
200
(
Total pages:
13
)
Abstract:
MicroRNAs (miRNAs) are a class of small RNAs, which typically function by guiding cleavage of target mRNAs. They are known to play roles in a variety of plant processes including development, responses to environmental stresses and senescence. To identify senescence regulation of miRNAs in Arabidopsis thaliana, eight small RNA libraries were constructed and sequenced at four different stages of development and senescence from both leaves and siliques, resulting in more than 200 million genome-matched sequences. Parallel analysis of RNA ends libraries, which enable the large-scale examination of miRNA-guided cleavage products, were constructed and sequenced, resulting in over 750 million genome-matched sequences. These large datasets led to the identification a new senescence-inducible small RNA locus, as well as new regulation of known miRNAs and their target genes during senescence, many of which have established roles in nutrient responsiveness and cell structural integrity. In keeping with remobilization of nutrients thought to occur during senescence, many miRNAs and targets had opposite expression pattern changes between leaf and silique tissues during the progression of senescence. Taken together, these findings highlight the integral role that miRNAs may play in the remobilization of resources and alteration of cellular structure that is known to occur in senescence. © 2014 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd.
Note:
Related Files :
arabidopsis
Arabidopsis thaliana
cell aging
Genetics
senescence
sequence analysis
Show More
Related Content
More details
DOI :
10.1111/pce.12393
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
20083
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:33
Scientific Publication
Differential expression of miRNAs and their target genes in senescing leaves and siliques: Insights from deep sequencing of small RNAs and cleaved target RNAs
38
Thatcher, S.R., Delaware Biotechnology Institute, University of Delaware, Newark, DE, United States, DuPont Pioneer, Wilmington, DE, United States
Burd, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Wright, C., Delaware Biotechnology Institute, University of Delaware, Newark, DE, United States, Princeton University, Princeton, NJ, United States
Lers, A., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Green, P.J., Delaware Biotechnology Institute, University of Delaware, Newark, DE, United States
Differential expression of miRNAs and their target genes in senescing leaves and siliques: Insights from deep sequencing of small RNAs and cleaved target RNAs
MicroRNAs (miRNAs) are a class of small RNAs, which typically function by guiding cleavage of target mRNAs. They are known to play roles in a variety of plant processes including development, responses to environmental stresses and senescence. To identify senescence regulation of miRNAs in Arabidopsis thaliana, eight small RNA libraries were constructed and sequenced at four different stages of development and senescence from both leaves and siliques, resulting in more than 200 million genome-matched sequences. Parallel analysis of RNA ends libraries, which enable the large-scale examination of miRNA-guided cleavage products, were constructed and sequenced, resulting in over 750 million genome-matched sequences. These large datasets led to the identification a new senescence-inducible small RNA locus, as well as new regulation of known miRNAs and their target genes during senescence, many of which have established roles in nutrient responsiveness and cell structural integrity. In keeping with remobilization of nutrients thought to occur during senescence, many miRNAs and targets had opposite expression pattern changes between leaf and silique tissues during the progression of senescence. Taken together, these findings highlight the integral role that miRNAs may play in the remobilization of resources and alteration of cellular structure that is known to occur in senescence. © 2014 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd.
Scientific Publication
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