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Heterozygote deficiency caused by a null allele at the bovine ARO23 microsatellite
Year:
1997
Source of publication :
Animal Biotechnology
Authors :
Ron, Micha
;
.
Volume :
8
Co-Authors:
Band, M., Institute of Animal Sciences, Volcani Center, Bet-Dagan 50250, Israel
Ron, M., Institute of Animal Sciences, Volcani Center, Bet-Dagan 50250, Israel
Facilitators :
From page:
187
To page:
190
(
Total pages:
4
)
Abstract:
Non-Mendelian inheritance and heterozygote deficiency were observed within the International Bovine Reference Panel (IBRP) when genotyped for AGC trinucleotide microsatellite ARO23. Chi square analysis showed a significant difference between the observed and predicted heterozygosity among 37 unrelated individuals. PCR reactions using an alternative primer designed to avoid a putative mismatch resulted in the appearance of an additional allele with a frequency of 0.30 and the restoration of Mendelian inheritance, Sequence analysis of this allele showed a cytosine insertion 2 bp from the 3′ end of the original priming site causing the failure of allele amplification. The presence of a segregating null allele may be suspected when heterozygote deficiency is observed.
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DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
20092
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:34
Scientific Publication
Heterozygote deficiency caused by a null allele at the bovine ARO23 microsatellite
8
Band, M., Institute of Animal Sciences, Volcani Center, Bet-Dagan 50250, Israel
Ron, M., Institute of Animal Sciences, Volcani Center, Bet-Dagan 50250, Israel
Heterozygote deficiency caused by a null allele at the bovine ARO23 microsatellite
Non-Mendelian inheritance and heterozygote deficiency were observed within the International Bovine Reference Panel (IBRP) when genotyped for AGC trinucleotide microsatellite ARO23. Chi square analysis showed a significant difference between the observed and predicted heterozygosity among 37 unrelated individuals. PCR reactions using an alternative primer designed to avoid a putative mismatch resulted in the appearance of an additional allele with a frequency of 0.30 and the restoration of Mendelian inheritance, Sequence analysis of this allele showed a cytosine insertion 2 bp from the 3′ end of the original priming site causing the failure of allele amplification. The presence of a segregating null allele may be suspected when heterozygote deficiency is observed.
Scientific Publication
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