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Angiotensin‐converting enzyme associated with Torpedo californica electric organ membranes
Year:
1987
Source of publication :
Journal of Neuroscience Research
Authors :
Altstein, Miriam
;
.
Volume :
18
Co-Authors:
Altstein, M., Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel
Dudai, Y., Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel
Vogel, Z., Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
333
To page:
340
(
Total pages:
8
)
Abstract:
Torpedo electric organ contains high concentrations of angiotensin converting enzyme (ACE) like activity, cleaving [Leu5]enkephalin at the Gly3‐Phe4 peptide bond. Most of the activity cosediments with the cell membranes. The enzymatic preparation from membranes is inhibited by low concentrations of the ACE inhibitors, AQ 14225 and SQ 20881 (IC50 of 0.6 and 15 nM, respectively), and is weakly inhibited by the neutral endopeptidase inhibitors, phosphoramidon and thiorphan (IC50 of 30 μM and ca. 70 nM, respectively). The enzyme degrades hippuryl‐His‐Leu and is activated by NaCl. Hippuryl‐His‐Leu and [Leu5]enkephalin are degraded with Km of 93 and 41 μM, and Vmax of 21 and 10 nmol/mg protein/min, respectively. The specific activity of the ACE‐like activity in homogenates of Torpedo electric organ is relatively high (6.3 nmol hippuryl‐His‐Leu/mg protein/min); this value is similar to that obtained for rat lung and rat striatum. Copyright © 1987 Alan R Liss, Inc.
Note:
Related Files :
Animal
animal experiment
cell membrane
fish
neutral endopeptidase
phosphoramidon
Show More
Related Content
More details
DOI :
10.1002/jnr.490180210
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
20107
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:34
Scientific Publication
Angiotensin‐converting enzyme associated with Torpedo californica electric organ membranes
18
Altstein, M., Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel
Dudai, Y., Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel
Vogel, Z., Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel
Angiotensin‐converting enzyme associated with Torpedo californica electric organ membranes
Torpedo electric organ contains high concentrations of angiotensin converting enzyme (ACE) like activity, cleaving [Leu5]enkephalin at the Gly3‐Phe4 peptide bond. Most of the activity cosediments with the cell membranes. The enzymatic preparation from membranes is inhibited by low concentrations of the ACE inhibitors, AQ 14225 and SQ 20881 (IC50 of 0.6 and 15 nM, respectively), and is weakly inhibited by the neutral endopeptidase inhibitors, phosphoramidon and thiorphan (IC50 of 30 μM and ca. 70 nM, respectively). The enzyme degrades hippuryl‐His‐Leu and is activated by NaCl. Hippuryl‐His‐Leu and [Leu5]enkephalin are degraded with Km of 93 and 41 μM, and Vmax of 21 and 10 nmol/mg protein/min, respectively. The specific activity of the ACE‐like activity in homogenates of Torpedo electric organ is relatively high (6.3 nmol hippuryl‐His‐Leu/mg protein/min); this value is similar to that obtained for rat lung and rat striatum. Copyright © 1987 Alan R Liss, Inc.
Scientific Publication
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