Co-Authors:
Zhang, N., Institute for Genomic Diversity, Cornell University, Ithaca, NY 14853, United States
Gibon, Y., Max Planck Institute of Molecular Plant Physiology, 14476 Golm-Potsdam, Germany, UMR 619 Fruit Biology, INRA Bordeaux, F-33883 Villenave d'Ornon, France
Gur, A., Institute for Genomic Diversity, Cornell University, Ithaca, NY 14853, United States, Teradion Industrial Park, Misgav 20179, Israel
Chen, C., Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853, United States
Lepak, N., United States Department of Agriculture, Agricultural Research Service, Robert W. Holley Center for Agriculture and Health, Ithaca, NY 14853, United States
Höhne, M., Max Planck Institute of Molecular Plant Physiology, 14476 Golm-Potsdam, Germany
Zhang, Z., Institute for Genomic Diversity, Cornell University, Ithaca, NY 14853, United States
Kroon, D., Institute for Genomic Diversity, Cornell University, Ithaca, NY 14853, United States
Tschoep, H., Max Planck Institute of Molecular Plant Physiology, 14476 Golm-Potsdam, Germany, Sesvanderhave N.V./S.A., Soldatenplein Z2 No. 15, 3300 Tienen, Belgium
Stitt, M., Max Planck Institute of Molecular Plant Physiology, 14476 Golm-Potsdam, Germany
Buckler, E., Institute for Genomic Diversity, Cornell University, Ithaca, NY 14853, United States, Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853, United States, United States Department of Agriculture, Agricultural Research Service, Robert W. Holley Center for Agriculture and Health, Ithaca, NY 14853, United States
Abstract:
Understanding the genetic basis of nitrogen and carbon metabolism will accelerate the development of plant varieties with high yield and improved nitrogen use efficiency. A robotized platform was used to measure the activities of 10 enzymes from carbon and nitrogen metabolism in the maize (Zea mays) intermated B73 × Mo17 mapping population, which provides almost a 4-fold increase in genetic map distance compared with conventional mapping populations. Seedling/juvenile biomass was included to identify its genetic factors and relationships with enzyme activities. All 10 enzymes showed heritable variation in activity. There were strong positive correlations between activities of different enzymes, indicating that they are coregulated. Negative correlations were detected between biomass and the activity of six enzymes. In total, 73 significant quantitative trait loci (QTL) were found that influence the activity of these 10 enzymes and eight QTL that influence biomass. While some QTL were shared by different enzymes or biomass, we critically evaluated the probability that this may be fortuitous. All enzyme activity QTL were in trans to the known genomic locations of structural genes, except for single cis-QTL for nitrate reductase, Glu dehydrogenase, and shikimate dehydrogenase; the low frequency and low additive magnitude compared with trans-QTL indicate that cis-regulation is relatively unimportant versus trans-regulation. Two-gene epistatic interactions were identified for eight enzymes and for biomass, with three epistatic QTL being shared by two other traits; however, epistasis explained on average only 2.8% of the genetic variance. Overall, this study identifies more QTL at a higher resolution than previous studies of genetic variation in metabolism. © 2010 American Society of Plant Biologists 1753.
תפריט נגישות
ניגודיות עדינה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
איפוס הגדרות נגישות
להורדת מודול נגישות חינם
