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Interactions of sendai virus with plant protoplasts
Year:
1985
Source of publication :
Plant Science
Authors :
Lavi, Uri
;
.
Salts, Yehiam
;
.
Volume :
41
Co-Authors:
Salts, Y., Department of Plant Genetics and Breeding, Department of Horticulture Genetics and Breeding, Agricultural Research Organization, Bet Dagan 50-250, Israel
Beckmann, J.S., Department of Plant Genetics and Breeding, Department of Horticulture Genetics and Breeding, Agricultural Research Organization, Bet Dagan 50-250, Israel
Loyter, A., Department of Plant Genetics and Breeding, Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel
Lavi, U., Department of Plant Genetics and Breeding, Department of Horticulture Genetics and Breeding, Agricultural Research Organization, Bet Dagan 50-250, Israel
Facilitators :
From page:
141
To page:
149
(
Total pages:
9
)
Abstract:
Sendai virus (SV), a fusogenic agent in animal cells, was investigated for its potential to interact and possibly fuse with the plasma membranes of plant protoplasts. Adsorption of fluorescently-labelled viral particles was observed to be dependent on the presence of concanavalin A (ConA). This interaction could not be reproduced by wheat germ agglutinin. ConA adsorption to protoplasts could be reversed by an excess of free competing sugar moieties (α-methyl-mannoside, αMM) at any time. Complexes between Sendai virions, ConA and protoplasts could be dissociated by αMM, only shortly after their formation. Prolonged incubation of these complexes prevented their dissociation upon the later addition of the competing sugar. Neither dithiothreitol nor trypsinization of the viruses, known to affect the viral fusogenic ability to animal cells, modified the described adsorption properties. This is taken to mean that irreversible binding of Sendai viral particles to protoplast plasma membranes does occur, presumably via a different mechanism from that with animal cells. This should encourage studies aimed at testing the capacity of the reconstituted Sendai envelopes to serve as delivery carriers in plant genetic engineering projects. © 1985.
Note:
Related Files :
concanavalin A
lectins
protoplast membranes
transformations
Show More
Related Content
More details
DOI :
10.1016/0168-9452(85)90116-5
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
20126
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:34
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Scientific Publication
Interactions of sendai virus with plant protoplasts
41
Salts, Y., Department of Plant Genetics and Breeding, Department of Horticulture Genetics and Breeding, Agricultural Research Organization, Bet Dagan 50-250, Israel
Beckmann, J.S., Department of Plant Genetics and Breeding, Department of Horticulture Genetics and Breeding, Agricultural Research Organization, Bet Dagan 50-250, Israel
Loyter, A., Department of Plant Genetics and Breeding, Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel
Lavi, U., Department of Plant Genetics and Breeding, Department of Horticulture Genetics and Breeding, Agricultural Research Organization, Bet Dagan 50-250, Israel
Interactions of sendai virus with plant protoplasts
Sendai virus (SV), a fusogenic agent in animal cells, was investigated for its potential to interact and possibly fuse with the plasma membranes of plant protoplasts. Adsorption of fluorescently-labelled viral particles was observed to be dependent on the presence of concanavalin A (ConA). This interaction could not be reproduced by wheat germ agglutinin. ConA adsorption to protoplasts could be reversed by an excess of free competing sugar moieties (α-methyl-mannoside, αMM) at any time. Complexes between Sendai virions, ConA and protoplasts could be dissociated by αMM, only shortly after their formation. Prolonged incubation of these complexes prevented their dissociation upon the later addition of the competing sugar. Neither dithiothreitol nor trypsinization of the viruses, known to affect the viral fusogenic ability to animal cells, modified the described adsorption properties. This is taken to mean that irreversible binding of Sendai viral particles to protoplast plasma membranes does occur, presumably via a different mechanism from that with animal cells. This should encourage studies aimed at testing the capacity of the reconstituted Sendai envelopes to serve as delivery carriers in plant genetic engineering projects. © 1985.
Scientific Publication
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