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Genetic changes during laboratory domestication of an olive fly SIT strain
Year:
2014
Source of publication :
Journal of Applied Entomology
Authors :
Nemny-Lavy, Esther
;
.
Nestel, David
;
.
Volume :
138
Co-Authors:
Zygouridis, N.E., Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece
Argov, Y., Citrus Division, Israel Cohen Institute for Biological Control, Plants Production and Marketing Board, Beit Dagan, Israel
Nemny-Lavy, E., Department of Entomology, Institute of Plant Protection, Agricultural Research Organization, Bet Dagan, Israel
Augustinos, A.A., Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece, Department of Biology, University of Patras, Patras, Greece
Nestel, D., Department of Entomology, Institute of Plant Protection, Agricultural Research Organization, Bet Dagan, Israel
Mathiopoulos, K.D., Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece
Facilitators :
From page:
423
To page:
432
(
Total pages:
10
)
Abstract:
Early attempts to apply the SIT on the olive fly, Bactrocera oleae, were unsuccessful, mainly due to the low competitiveness of the sterile mass-reared males compared with the wild ones. Recently, new efforts are underway in Israel to develop a vigorous and efficient mass-reared olive fly laboratory strain. To understand the genetics of the adaptation process and possibly link the corresponding loss of competitiveness to genetic markers, we followed the fluctuation of genotypic frequencies of ten microsatellite markers during the course of 22 generations of the colonization of a wild Israeli population in laboratory conditions. Effective and observed allele number is halved after 11 generations, so is mean heterozygosity. Practically, there is very little change between F0 and F1, there are substantial changes between F1 and F2-F5, and there is a virtual complete adaptation to the new laboratory environment by F11, because no more changes are observed between F11 and F22. If we assume that the loss of allele number and heterozygosity also reflects the loss of the 'wild' character of the colonized strain and, possibly, the loss of a substantial part of its natural vigour, our results indicate that there is an apparent need to refresh a mass-reared colony with wild material at about every five to eight generations. Furthermore, simulation models indicated that while most of the observed allele frequency fluctuations were due to random drift, some alleles were probably under selection. © 2013 Blackwell Verlag GmbH.
Note:
Related Files :
adaptation
Bactrocera oleae
colonization
Drift
fly
genetic drift
laboratory method
Sterile insect technique
sterile release method
Show More
Related Content
More details
DOI :
10.1111/jen.12042
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
20139
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:34
You may also be interested in
Scientific Publication
Genetic changes during laboratory domestication of an olive fly SIT strain
138
Zygouridis, N.E., Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece
Argov, Y., Citrus Division, Israel Cohen Institute for Biological Control, Plants Production and Marketing Board, Beit Dagan, Israel
Nemny-Lavy, E., Department of Entomology, Institute of Plant Protection, Agricultural Research Organization, Bet Dagan, Israel
Augustinos, A.A., Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece, Department of Biology, University of Patras, Patras, Greece
Nestel, D., Department of Entomology, Institute of Plant Protection, Agricultural Research Organization, Bet Dagan, Israel
Mathiopoulos, K.D., Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece
Genetic changes during laboratory domestication of an olive fly SIT strain
Early attempts to apply the SIT on the olive fly, Bactrocera oleae, were unsuccessful, mainly due to the low competitiveness of the sterile mass-reared males compared with the wild ones. Recently, new efforts are underway in Israel to develop a vigorous and efficient mass-reared olive fly laboratory strain. To understand the genetics of the adaptation process and possibly link the corresponding loss of competitiveness to genetic markers, we followed the fluctuation of genotypic frequencies of ten microsatellite markers during the course of 22 generations of the colonization of a wild Israeli population in laboratory conditions. Effective and observed allele number is halved after 11 generations, so is mean heterozygosity. Practically, there is very little change between F0 and F1, there are substantial changes between F1 and F2-F5, and there is a virtual complete adaptation to the new laboratory environment by F11, because no more changes are observed between F11 and F22. If we assume that the loss of allele number and heterozygosity also reflects the loss of the 'wild' character of the colonized strain and, possibly, the loss of a substantial part of its natural vigour, our results indicate that there is an apparent need to refresh a mass-reared colony with wild material at about every five to eight generations. Furthermore, simulation models indicated that while most of the observed allele frequency fluctuations were due to random drift, some alleles were probably under selection. © 2013 Blackwell Verlag GmbH.
Scientific Publication
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