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Interaction of the bacteriocin pediocin SJ-1 with the cytoplasmic membrane of sensitive bacterial cells as detected by ANS fluorescence
Year:
1994
Source of publication :
Journal of Applied Bacteriology
Authors :
Lindner, Pinhas
;
.
Schved, Fernando
;
.
Volume :
76
Co-Authors:
Schved, F., ITSAP, Agricultural Research Organization, Volcani Centre, P.O. Box 6, Bet Dagan 50250, Israel
Lindner, P., ITSAP, Agricultural Research Organization, Volcani Centre, P.O. Box 6, Bet Dagan 50250, Israel
Juven, B.J., ITSAP, Agricultural Research Organization, Volcani Centre, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
30
To page:
35
(
Total pages:
6
)
Abstract:
The 1-anilino-8-naphthalenesulphonic acid (ANS) fluorescent probe was used to monitor alterations in the cytoplasmic membrane of sensitive Lactobacillus plantarum cells, caused by pediocin SJ-1, a bacteriocin produced by Pediococcus acidilactici. The addition of pediocin SJ-1 to the sensitive cells caused an increase in fluorescence intensity of ANS and a blue shift in its emission maximum from 520 to 475 nm. None of these spectral changes could be detected when pediocin SJ-1 was added to cells of a Lact. plantarum variant resistant to pediocin SJ-1. Upon the addition of pediocin SJ-1, dose- dependent energy transfer took place between tryptophanyl residues in the cytoplasmic membrane of sensitive cells and ANS. Similar ANS-fluorescence changes were observed with the bacteriocin nisin. The concentrations of pediocin SJ-1 needed to effect changes in the fluorescence spectrum of ANS were of the same magnitude as those required for a bactericidal effect and the release of u.v.-absorbing material. A hypothesis on the mode of action of pediocin SJ-1 is proposed.
Note:
Related Files :
bacterial growth
bacteriocin
cell viability
Fluorescence
Lactobacillus plantarum
nisin
pediocin sj 1
tryptophan
unclassified drug
Show More
Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
20213
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:34
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Scientific Publication
Interaction of the bacteriocin pediocin SJ-1 with the cytoplasmic membrane of sensitive bacterial cells as detected by ANS fluorescence
76
Schved, F., ITSAP, Agricultural Research Organization, Volcani Centre, P.O. Box 6, Bet Dagan 50250, Israel
Lindner, P., ITSAP, Agricultural Research Organization, Volcani Centre, P.O. Box 6, Bet Dagan 50250, Israel
Juven, B.J., ITSAP, Agricultural Research Organization, Volcani Centre, P.O. Box 6, Bet Dagan 50250, Israel
Interaction of the bacteriocin pediocin SJ-1 with the cytoplasmic membrane of sensitive bacterial cells as detected by ANS fluorescence
The 1-anilino-8-naphthalenesulphonic acid (ANS) fluorescent probe was used to monitor alterations in the cytoplasmic membrane of sensitive Lactobacillus plantarum cells, caused by pediocin SJ-1, a bacteriocin produced by Pediococcus acidilactici. The addition of pediocin SJ-1 to the sensitive cells caused an increase in fluorescence intensity of ANS and a blue shift in its emission maximum from 520 to 475 nm. None of these spectral changes could be detected when pediocin SJ-1 was added to cells of a Lact. plantarum variant resistant to pediocin SJ-1. Upon the addition of pediocin SJ-1, dose- dependent energy transfer took place between tryptophanyl residues in the cytoplasmic membrane of sensitive cells and ANS. Similar ANS-fluorescence changes were observed with the bacteriocin nisin. The concentrations of pediocin SJ-1 needed to effect changes in the fluorescence spectrum of ANS were of the same magnitude as those required for a bactericidal effect and the release of u.v.-absorbing material. A hypothesis on the mode of action of pediocin SJ-1 is proposed.
Scientific Publication
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